公开(公告)号：US20210340593A1

公开(公告)日：2021-11-04

申请号：US17374460

申请日：2021-07-13

Applicant: Promega Corporation

Inventor： Carolyn W. Hitko ,  Thomas Kirkland ,  Sergiy Levin ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Harry Tetsuo Uyeda ,  Ji Zhu

IPC: C12Q1/34 ,  G01N33/543 ,  C12N9/14

Abstract: The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.

公开(公告)号：US09593316B2

公开(公告)日：2017-03-14

申请号：US14285327

申请日：2014-05-22

Applicant: Promega Corporation

Inventor： Aldis Darzins ,  Lance P. Encell ,  Rachel Friedman Ohana ,  Paul Otto ,  Michael R. Slater ,  Gediminas Vidugiris ,  Keith V. Wood ,  Monika G. Wood ,  Kate Qin Zhao ,  Kris Zimmerman

IPC: C07H21/00 ,  C12N1/21 ,  C12N1/15 ,  C12N9/14 ,  C12Q1/34 ,  G01N33/58

CPC classification number: C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

Abstract translation: 本发明提供具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸以及使用多核苷酸和突变蛋白的方法。

公开(公告)号：US20140194325A1

公开(公告)日：2014-07-10

申请号：US14104845

申请日：2013-12-12

Applicant: Promega Corporation

Inventor： Carolyn W. Hitko ,  Thomas Kirkland ,  Thomas Machleidt ,  Rachel Friedman Ohana ,  Matt Robers ,  Keith Wood

IPC: G01N33/58 ,  G01N33/94

CPC classification number: G01N33/94 ,  G01N33/542 ,  G01N33/582

Abstract: The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.

Abstract translation: 本发明提供用于检测和分析生物活性剂与细胞靶标的细胞内结合的组合物和方法。 特别地，本文提供的是连接于荧光团的生物活性剂，与生物发光报道融合的细胞靶标，以及检测和分析生物活性剂与细胞靶标的相互作用的方法。

公开(公告)号：US20240132859A1

公开(公告)日：2024-04-25

申请号：US18312441

申请日：2023-05-04

Applicant: Promega Corporation

Inventor： Michael P. Killoran ,  Lance P. Encell ,  Thomas Kirkland ,  Thomas Machleidt ,  Rachel Friedman Ohana ,  Robin Hurst ,  Mark A. Klein ,  Karilyn Porter ,  Rahele Esmatpour ,  Debayan De Bakshi

IPC: C12N9/14

CPC classification number: C12N9/14 ,  C12Y308/01

Abstract: Provided herein modified dehalogenases have extended surface loop regions that provide a location for internal fusion insertions and modulate binding interaction and activation of environmentally-sensitive chemistries.

公开(公告)号：US20230357266A1

公开(公告)日：2023-11-09

申请号：US18312418

申请日：2023-05-04

Applicant: Promega Corporation

Inventor： Matthew A. Larsen ,  Rachel Friedman Ohana ,  Wenhui Zhou ,  Ce Shi ,  Jian Cao ,  Robin Hurst ,  Mark A. Klein ,  Hui Wang ,  Weiwei An ,  Karilyn Porter ,  Thomas Machleidt

IPC: C07D495/04 ,  B01J23/46 ,  B01J35/00 ,  C07B59/00

CPC classification number: C07D495/04 ,  B01J23/468 ,  B01J23/462 ,  B01J35/004 ,  C07B59/002 ,  C07B2200/05

Abstract: Provided herein are compounds, compositions, systems, and methods for photoactivatable labeling, which can be actuated within biological systems. In particular, compounds disclosed herein include vinyl-extended-aryl azide moieties that undergo photoactivation to generate reactive intermediates, which can form covalent linkages with biomolecules. The photoactivation can be conducted by a variety of mechanisms including ultraviolet (UV) irradiation, visible light irradiation, or energy transfer (e.g., from a photocatalyst). The compounds also include functional moieties that provide useful functionalities, for example detection of biomolecules, such as fluorophores, capture elements (e.g., biotin), or reactive moieties (e.g., click handles) and bifunctional moieties comprising a bioactive compound and a detection/capture element.

公开(公告)号：US20200340982A1

公开(公告)日：2020-10-29

申请号：US16825802

申请日：2020-03-20

Applicant: Promega Corporation

Inventor： Sergiy Levin ,  Rachel Friedman Ohana ,  Robin Hurst ,  Thomas Kirkland ,  Keith Wood

IPC: G01N33/533 ,  G01N21/76 ,  G01N33/542 ,  G01N21/64

Abstract: Provided herein are compositions and methods for photoaffinity labeling of molecular targets. In particular, probes that specifically interact with cellular targets based on their affinity and are then covalently linked to the cellular target via a photoreactive group (PRG) on the probe.

公开(公告)号：US10067149B2

公开(公告)日：2018-09-04

申请号：US14104845

申请日：2013-12-12

Applicant: Promega Corporation

Inventor： Carolyn W. Hitko ,  Thomas Kirkland ,  Thomas Machleidt ,  Rachel Friedman Ohana ,  Matt Robers ,  Keith Wood

IPC: G01N33/94 ,  G01N33/542 ,  G01N33/58

Abstract: The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.

公开(公告)号：US20170137793A1

公开(公告)日：2017-05-18

申请号：US15414291

申请日：2017-01-24

Applicant: Promega Corporation

Inventor： Aldis Darzins ,  Lance P. Encell ,  Rachel Friedman Ohana ,  Paul Otto ,  Michael R. Slater ,  Gediminas Vidugiris ,  Keith V. Wood ,  Monika G. Wood ,  Kate Qin Zhao ,  Kris Zimmerman

IPC: C12N9/14 ,  C12N9/96 ,  C12N11/00

CPC classification number: C12N9/14 ,  C12N9/96 ,  C12N11/00 ,  C12Q1/34 ,  C12Y308/01 ,  G01N33/581

Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

公开(公告)号：US20170115283A1

公开(公告)日：2017-04-27

申请号：US15402879

申请日：2017-01-10

Applicant: Promega Corporation

Inventor： Carolyn W. Hitko ,  Robin Hurst ,  Thomas Kirkland ,  Dieter Klaubert ,  Poncho Meisenheimer ,  Nidhi Nath ,  Rachel Friedman Ohana ,  Paul Otto ,  Marjeta Urh ,  Harry Tetsuo Uyeda ,  Keith Wood ,  Ji Zhu

IPC: G01N33/543

CPC classification number: G01N33/54333 ,  G01N33/54306 ,  G01N33/54326

Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.

公开(公告)号：US20140322738A1

公开(公告)日：2014-10-30

申请号：US14207959

申请日：2014-03-13

Applicant: PROMEGA CORPORATION

Inventor： Carolyn W. Hitko ,  Thomas Kirkland ,  Sergiy Levin ,  Poncho Meisenheimer ,  Rachel Friedman Ohana ,  Harry Tetsuo Uyeda ,  Ji Zhu

IPC: C12Q1/34

CPC classification number: C12Q1/34 ,  C12N9/14 ,  G01N33/54353

Abstract: The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.

Abstract translation: 本发明提供了卤代烷烃基材和用于将这些基材连接到功能元件（例如标签，标签，表面等）上的连接器。 本文所述的底物和接头可用于例如蛋白质，细胞和分子的标记，检测和固定。 特别地，本文提供的接头在底物中用于与其卤代烷基底物形成共价键的脱卤酶变体。