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公开(公告)号：US20240132859A1
公开(公告)日：2024-04-25
申请号：US18312441
申请日：2023-05-04
Applicant: Promega Corporation
Inventor： Michael P. Killoran , Lance P. Encell , Thomas Kirkland , Thomas Machleidt , Rachel Friedman Ohana , Robin Hurst , Mark A. Klein , Karilyn Porter , Rahele Esmatpour , Debayan De Bakshi
IPC: C12N9/14
CPC classification number: C12N9/14 , C12Y308/01
Abstract: Provided herein modified dehalogenases have extended surface loop regions that provide a location for internal fusion insertions and modulate binding interaction and activation of environmentally-sensitive chemistries.
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![SUBSTITUTED IMIDAZO[1,2-a]PYRAZINES AS LUCIFERASE SUBSTRATES](/abs-image/US/2024/02/29/US20240068009A1/abs.jpg.150x150.jpg)
公开(公告)号：US20240068009A1
公开(公告)日：2024-02-29
申请号：US18457868
申请日：2023-08-29
Applicant: Promega Corporation
Inventor： Anton Shakhmin , Thomas Kirkland , Joel Walker , Thomas Machleidt , Mary Hall , Keith V. Wood
IPC: C12Q1/66 , A61K49/00 , C07D487/04 , C07D519/00 , G01N33/58 , H04N1/60
CPC classification number: C12Q1/66 , A61K49/0013 , C07D487/04 , C07D519/00 , G01N33/581 , H04N1/6041 , G01N2333/90241
Abstract: Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods from making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.
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公开(公告)号：US20230341410A1
公开(公告)日：2023-10-26
申请号：US18150018
申请日：2023-01-04
Applicant: Promega Corporation
Inventor： Melanie Dart , Lance P. Encell , Thomas Kirkland , Thomas Machleidt , Matthew Robers , Brock F. Binkowski , Keith Wood , Ce Shi
IPC: G01N33/68 , G01N33/569 , G01N21/64 , G01N33/58
CPC classification number: G01N33/6845 , G01N33/56916 , C12Y113/12007 , G01N21/6428 , G01N33/581 , G01N33/582 , G01N2021/6432 , G01N2021/6439 , G01N2333/90241 , G01N2500/04 , G01N2500/10
Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
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公开(公告)号：US11567083B2
公开(公告)日：2023-01-31
申请号：US17105024
申请日：2020-11-25
Applicant: Promega Corporation
Inventor： Melanie Dart , Lance P. Encell , Thomas Kirkland , Thomas Machleidt , Matthew Robers , Brock F. Binkowski , Keith Wood , Ce Shi
IPC: G01N33/68 , G01N33/58 , G01N21/64 , G01N33/569
Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
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5.发明授权公开(公告)号：US11261478B2
公开(公告)日：2022-03-01
申请号：US16335638
申请日：2017-10-13
Applicant: Promega Corporation
Inventor： Ce Shi , Thomas Kirkland , Poncho Meisenheimer , Lance P. Encell , Mary Hall
Abstract: Provided herein are inhibitor-resistant luciferase mutants, and methods of use thereof. In particular, luciferase mutants are provided that are thermal stable and exhibit improved stability in the presence of luciferin break-down products, such as dehydroluciferin. Further provided are assay systems comprising inhibitor-resistant luciferase mutants and amino acid sequences of the inhibitor-resistant luciferase mutants.
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Patent Agency Ranking
公开(公告)号：US20210080470A1
公开(公告)日：2021-03-18
申请号：US17105024
申请日：2020-11-25
Applicant: Promega Corporation
Inventor： Melanie Dart , Lance P. Encell , Thomas Kirkland , Thomas Machleidt , Matthew Robers , Brock F. Binkowski , Keith Wood , Ce Shi
IPC: G01N33/68 , G01N33/569 , G01N21/64 , G01N33/58
Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
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公开(公告)号：US10928400B2
公开(公告)日：2021-02-23
申请号：US16787950
申请日：2020-02-11
Applicant: Promega Corporation
Inventor： Melanie Dart , Lance P. Encell , Thomas Kirkland , Thomas Machleidt , Matthew Robers , Brock F. Binkowski , Keith Wood , Ce Shi
Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
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公开(公告)号：US20200340982A1
公开(公告)日：2020-10-29
申请号：US16825802
申请日：2020-03-20
Applicant: Promega Corporation
Inventor： Sergiy Levin , Rachel Friedman Ohana , Robin Hurst , Thomas Kirkland , Keith Wood
IPC: G01N33/533 , G01N21/76 , G01N33/542 , G01N21/64
Abstract: Provided herein are compositions and methods for photoaffinity labeling of molecular targets. In particular, probes that specifically interact with cellular targets based on their affinity and are then covalently linked to the cellular target via a photoreactive group (PRG) on the probe.
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公开(公告)号：US20190389869A1
公开(公告)日：2019-12-26
申请号：US16545903
申请日：2019-08-20
Applicant: PROMEGA CORPORATION
Inventor： Mary Hall , Thomas Kirkland , Thomas Machleidt , Anton Shakhmin , Joel R. Walker
IPC: C07D487/04 , C12Q1/66 , C09K11/06 , C07D519/00 , A61K49/00
Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
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公开(公告)号：US20190241932A1
公开(公告)日：2019-08-08
申请号：US16390382
申请日：2019-04-22
Applicant: PROMEGA CORPORATION
Inventor： Anton Shakhmin , Thomas Kirkland , Joel Walker , Thomas Machleidt , Mary Hall , Keith V. Wood
IPC: C12Q1/66 , H04N1/60 , G01N33/58 , C07D487/04 , A61K49/00 , C07D519/00
CPC classification number: C12Q1/66 , A61K49/0013 , C07D487/04 , C07D519/00 , G01N33/581 , G01N2333/90241 , H04N1/6041
Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
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