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    NOVEL FLUORESCENCE-BASED ASSAY FOR THE RAPID DETECTION AND QUANTIFICATION OF DEOXYRIBONUCLEOSIDE TRIPHOSPHATES
    2.
    发明申请
    NOVEL FLUORESCENCE-BASED ASSAY FOR THE RAPID DETECTION AND QUANTIFICATION OF DEOXYRIBONUCLEOSIDE TRIPHOSPHATES 有权
    用于快速检测和定量脱氧核糖核苷二磷酸的新型基于荧光的测定

    公开(公告)号：US20130029331A1

    公开(公告)日：2013-01-31

    申请号：US13523782

    申请日：2012-06-14

    申请人： Peter M. Wilson ,  Robert D. Ladner

    发明人： Peter M. Wilson ,  Robert D. Ladner

    IPC分类号： G01N21/64 ,  C07H21/00

    CPC分类号： C12Q1/6844 ,  C12Q1/6823 ,  C12Q1/6825 ,  G01N21/6428 ,  C12Q2521/319 ,  C12Q2565/1025 ,  C12Q2565/102

    摘要： The inventors have developed a rapid and sensitive fluorescence-based assay to quantify dNTPs. This assay relies on the principle that incorporation of a limiting dNTP is required for primer-extension and polymerase-mediated 5-3′ exonuclease hydrolysis of a quenched fluorophore-labeled probe resulting in fluorescence. The concentration of limiting dNTPs is directly proportional to the fluorescence generated. This assay has important applications in research that investigates the influence of pathological conditions or pharmacological agents on dNTP biosynthesis and regulation.

    摘要翻译： 本发明人已经开发了一种快速和灵敏的基于荧光的测定来定量dNTP。 该测定依赖于引入限制性dNTP的引物延伸和聚合酶介导的5-3'外切核酸酶水解淬灭的荧光团标记的探针导致荧光的原理。 限制性dNTPs的浓度与产生的荧光成正比。 该研究在研究病理学条件或药理作用对dNTP生物合成和调节的影响方面具有重要应用。

    Device and method for safe location and marking of a biopsy cavity
    10.
    发明授权
    Device and method for safe location and marking of a biopsy cavity 有权
    安全定位和标记活检腔的装置和方法

    公开(公告)号：US09492570B2

    公开(公告)日：2016-11-15

    申请号：US12777710

    申请日：2010-05-11

    申请人： D. Laksen Sirimanne ,  Natalie V. Fawzi ,  Douglas S. Sutton ,  Gail S. Lebovic ,  Stanley R. Conston ,  Peter M. Wilson ,  Anne B. Morrissey ,  Mary Elizabeth Bush

    发明人： D. Laksen Sirimanne ,  Natalie V. Fawzi ,  Douglas S. Sutton ,  Gail S. Lebovic ,  Stanley R. Conston ,  Peter M. Wilson ,  Anne B. Morrissey ,  Mary Elizabeth Bush

    IPC分类号： A61B6/00 ,  A61K49/00 ,  A61B17/00

    CPC分类号： A61K49/006 ,  A61B90/39 ,  A61B2017/00539 ,  A61B2090/3925 ,  A61B2090/3966 ,  A61B2090/3987 ,  A61B2090/3995

    摘要： Cavity and sentinel lymph node marking devices, marker delivery devices, and methods are disclosed. More particularly, upon insertion into a body, the cavity marking device and method enable one to determine the center, orientation, and periphery of the cavity by radiographic, mammography, echogenic, or other noninvasive imaging techniques. A composition and method are disclosed for locating the sentinel lymph node in a mammalian body to determine if cancerous cells have spread thereto. The composition is preferably a fluid composition consisting of a carrier fluid and some type of contrast agent; alternatively, the contrast agent may itself be a fluid and therefore not need a separate carrier fluid. This composition is capable of (1) deposition in or around a lesion and migration to and accumulation in the associated sentinel node, and (2) remote detection via any number of noninvasive techniques. Also disclosed is a method for remotely detecting the location of a sentinel node by (1) depositing a remotely detectable fluid in or around a lesion for migration to and accumulation in the associated sentinel node and (2) remotely detecting the location of that node with a minimum of trauma and toxicity to the patient. The composition and method may serve to mark a biopsy cavity, as well as mark the sentinel lymph node. The marking methods also may combine any of the features as described with the marking device and delivery device.

    摘要翻译： 公开了腔和前哨淋巴结标记装置，标记递送装置和方法。 更具体地说，在插入体中时，腔标记装置和方法使得能够通过放射照相，乳房X线照相术，回声或其它非侵入性成像技术确定腔的中心，取向和周边。 公开了用于在哺乳动物体内定位前哨淋巴结的组合物和方法，以确定癌细胞是否已扩散到其上。 组合物优选是由载体流体和某种类型的造影剂组成的流体组合物; 或者，造影剂本身可以是流体，因此不需要单独的载体流体。 该组合物能够（1）沉积在病变中或周围，并且迁移到相关的前哨淋巴结中并累积在相关的前哨淋巴结中，以及（2）通过任何数量的无创技术的远程检测。 还公开了一种用于通过以下步骤远程检测前哨节点的位置的方法：（1）在病变中或周围沉积可远程检测的流体，以便迁移到相关联的前哨节点并累积在相关联的哨兵节点中;以及（2）远程检测该节点的位置， 对患者的至少创伤和毒性。 组合物和方法可用于标记活检腔，以及标记前哨淋巴结。 标记方法还可以将所描述的特征与标记装置和递送装置组合。