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公开(公告)号：US20210341718A1
公开(公告)日：2021-11-04
申请号：US17242377
申请日：2021-04-28
Applicant: Leica Microsystems CMS GmbH
Inventor： Florian FAHRBACH
Abstract: A light sheet microscope includes an object slide, and optical illumination and detection systems. The optical illumination system includes an illumination objective configured to illuminate a first object plane that is oblique relative to a slide plane with a light sheet. The optical detection system includes a detection objective and an image sensor device. The image sensor device is configured to define a first image plane which is orthogonal to an optical axis of the detection objective and to define a second image plane which is tilted relative to the first image plane. The detection objective is configured to image a focal plane onto the first image plane and to image a second object plane of the object onto the second image plane, the focal plane being coincident with the first object plane, and the second object plane being parallel to or coincident with the slide plane.
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2.发明申请公开(公告)号：US20190086656A1
公开(公告)日：2019-03-21
申请号：US16064533
申请日：2016-12-23
Applicant: Leica Microsystems CMS GmbH
Inventor： Florian FAHRBACH , Werner KNEBEL
Abstract: A method for examining a sample by light sheet microscopy includes illuminating a sample surface located in an illumination plane by a light sheet propagating in the illumination plane. A position of a light sheet focal point of the light sheet in the illumination plane is moved by changing an optical length of a light path of illumination light forming the light sheet. Detection light emanating from the illumination plane is detected.
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3.发明公开公开(公告)号：US20230314783A1
公开(公告)日：2023-10-05
申请号：US18020097
申请日：2021-08-16
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Florian FAHRBACH
IPC: G02B21/06
CPC classification number: G02B21/06
Abstract: An optical device for a microscope includes a reflector assembly including a beam entrance, at which a light beam can be coupled into the reflector assembly, including a beam exit, and including a beam path extending within the reflector assembly. The beam path begins in the reflector assembly at the beam entrance and passes the beam exit multiple times. On each passage of the beam path, partial beams are coupled out of the reflector assembly at the beam exit. The partial beams coupled out at the beam exit either extend in parallel and so as to be spaced apart from one another or are directed out of the reflector assembly starting from a common starting point.
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4.发明公开公开(公告)号：US20230314780A1
公开(公告)日：2023-10-05
申请号：US18041227
申请日：2021-08-12
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Florian FAHRBACH
CPC classification number: G02B21/0032 , G02B21/0076 , G02B21/367
Abstract: An optical apparatus for production of light sheets includes an optical splitter system which, at an optical splitter system output, includes a plurality of collimated light beams converging on a common point; a retroreflector, including a beam input at which the plurality of light beams are coupled into the retroreflector, and a beam output at which the plurality of light beams are emitted from the retroreflector; and a light sheet optical system into which the plurality of light beams emitted from the beam output of the retroreflector extend. At a light sheet optical system output, a differently oriented light sheet is formed from each light beam. A path length covered within the retroreflector by the plurality of light beams between the beam input and the beam output is dependent on an angle of incidence of each light beam at the beam input.
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公开(公告)号：US20230086623A1
公开(公告)日：2023-03-23
申请号：US17939064
申请日：2022-09-07
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Florian FAHRBACH , Sebastian SIMMER , Stefan CHRIST , Nariman ANSARI
Abstract: A microscope system includes a microscope stage having a top surface configured to have a sample carrier arranged thereon, the sample carrier being configured to receive at least one sample. The microscope system also includes an imaging system configured to image the at least one sample. The microscope system also includes an injector configured to inject a predetermined amount of a liquid into the sample carrier by injecting multiple successive and temporally spaced jets of the liquid into the sample carrier, each jet including a predetermined portion of the amount of liquid.
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Patent Agency Ranking
公开(公告)号：US20210172876A1
公开(公告)日：2021-06-10
申请号：US17110455
申请日：2020-12-03
Applicant: Leica Microsystems CMS GmbH
Inventor： Florian FAHRBACH
Abstract: A light sheet fluorescence microscope includes a light source configured to emit excitation light suitable for inducing fluorescent light emitted from a specimen, a detector configured to detect the fluorescent light from the specimen, and an optical system configured to illuminate the specimen with a light sheet formed from the excitation light, and to guide the fluorescent light from the illuminated specimen to the detector. The optical system comprises an objective facing the specimen, the objective being configured to collect the fluorescent light emitted from the specimen. The light source is further configured to emit manipulation light suitable for photomanipulating the specimen. The optical system is further configured to direct the manipulation light through a spatially limited sub-area of an entrance pupil of the objective onto the specimen along a light propagation direction which is different from a light propagation direction of the light sheet.
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公开(公告)号：US20210088767A1
公开(公告)日：2021-03-25
申请号：US17020885
申请日：2020-09-15
Applicant: Leica Microsystems CMS GmbH
Inventor： Florian FAHRBACH
IPC: G02B21/00
Abstract: A light sheet microscope, such as an oblique plane microscope or a swept confocally-aligned planar excitation (SCAPE) microscope, includes an optical system with a first optical element, a second optical element and a mirror assembly. In a first operational state of the microscope, the optical system is configured to transmit illumination light along a first illumination light path through the first optical element into a first sample volume, and configured to transmit observation light along a first observation light path from the first sample volume to a detector device. In a second operational state of the microscope, the optical system is configured to transmit the illumination light along a second illumination light path via the mirror assembly through the second optical element into a second sample volume, and configured to transmit observation light along a second observation light path from the second sample volume to the detector device.
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公开(公告)号：US20210072524A1
公开(公告)日：2021-03-11
申请号：US17040574
申请日：2019-03-14
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Florian FAHRBACH
IPC: G02B21/00
Abstract: The invention relates to an optical system (1) comprising an illumination device (7) for generating illumination light (13), orientation optics (17) and a scanning device (49) for generating a temporal sequence of at least two tilted illumination planes (29) for illuminating a stationary sample volume (33). The invention further relates to a method for illuminating a sample volume (33). Optical systems (1) and methods in prior art have the disadvantage of a complex beam path and said systems and methods achieve a non-uniform illumination of the sample. The optical system (1) according to the invention improves on the solutions in prior art in that the at least two tilted illumination planes (29) are parallel to one another. In the method according to the invention, temporally successive illumination planes (29) are offset parallel to one another by a scanning device (49).
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公开(公告)号：US20180180864A1
公开(公告)日：2018-06-28
申请号：US15566729
申请日：2016-04-18
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Werner KNEBEL , Frank SIECKMANN , Florian FAHRBACH
CPC classification number: G02B21/006 , G02B21/0032 , G02B21/18 , G02B21/248 , G02B21/367
Abstract: A method for analyzing a microscopic sample with a microscope includes illuminating at least a sub-region of the microscopic sample by illumination light. Detection light emanating from the microscopic sample is guided on a detection beam path, which includes at least one focusing optical element and which has a plurality of detection beam path branches, each with at least one detector element. The detector elements are parts of the same surface detector. By art adjusting element in at least a first one of the detection beam path branches, an optical path length of the first detection beam path branch is adjusted in such a way that the portion of the detection light guided on the first detection beam path branch is focused on the detector element of the first detection beam path branch.
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公开(公告)号：US20230185068A1
公开(公告)日：2023-06-15
申请号：US17917003
申请日：2021-04-08
Applicant: LEICA MICROSYSTEMS CMS GMBH
Inventor： Florian FAHRBACH
CPC classification number: G02B21/0032 , G02B21/0076 , G02B21/367
Abstract: An inclined plane microscope includes a detection assembly for collecting scattered light and/or fluorescent light from regions of a sample volume illuminated by a light sheet, wherein the detection assembly, on its side facing away from the sample volume, is configured to image a real intermediate image of the regions of the sample illuminated by the light sheet, and wherein the real intermediate image is tilted in relation to a focal plane of the detection assembly, and an optical alignment unit for imaging a predetermined image field of the real intermediate image onto a detector, wherein the alignment unit has a depth of field region, wherein a focal plane of the alignment unit is tilted in relation to the real intermediate image, and wherein the predetermined image field of the real intermediate image to be imaged by the alignment unit is located completely within the depth of field region.
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