公开(公告)号：US5083283A

公开(公告)日：1992-01-21

申请号：US323490

申请日：1989-03-14

申请人： Kyoko Imai ,  Yasushi Nomura ,  Hiroshi Umetsu

发明人： Kyoko Imai ,  Yasushi Nomura ,  Hiroshi Umetsu

IPC分类号： G01N33/53 ,  G01N21/27 ,  G01N33/536 ,  G01N33/543 ,  G01N35/00 ,  G12B13/00

CPC分类号： G01N21/274 ,  G12B13/00 ,  G01N33/53 ,  G01N35/00

摘要： A method of determining a calibration curve used in deciding the components of living organism by means of the least-squares method by using a plurality of measured data at different concentrations obtained by measuring reaction solutions of a plurality of standard substances having different concentrations. At least a single set of measured data Y(i) is weighted in a predetermined manner, the measured data Y(i) being obtained from the standard substance reaction solution at a specific concentration X(i) near a limit value used in deciding the living organism components. With the weighting, a calibration curve having a high precision at a portion near the limit value is realized resulting in a correct and highly reliable decision.

摘要翻译： 通过使用通过测量具有不同浓度的多种标准物质的反应溶液获得的不同浓度的多个测量数据，通过最小二乘法确定用于决定活生物体组分的校准曲线的方法。 至少一组测量数据Y（i）以预定的方式加权，测量数据Y（i）从标准物质反应溶液获得，其特定浓度X（i）接近用于决定 活体组分。 通过加权，实现在接近极限值的部分具有高精度的校准曲线，从而得到正确和高度可靠的决定。

公开(公告)号：US5272092A

公开(公告)日：1993-12-21

申请号：US793650

申请日：1991-11-18

申请人： Fumitoshi Hamasaki ,  Hajime Betsui ,  Kyoko Imai ,  Hiroshi Umetsu

发明人： Fumitoshi Hamasaki ,  Hajime Betsui ,  Kyoko Imai ,  Hiroshi Umetsu

IPC分类号： B01F11/00 ,  B01F13/08 ,  G01N21/25 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  B01F3/08 ,  G01N21/76

CPC分类号： B01F11/0005 ,  B01F13/0818 ,  G01N21/253 ,  G01N35/025 ,  G01N2035/00435 ,  G01N2035/00534 ,  G01N2035/0448 ,  G01N35/1079

摘要： A plurality of reaction containers each being charged with an agitation ball made of a magnetic material are circularly arranged on a reaction disk so as to be supplied with a reagent and a sample. The reaction disk is driven reciprocatingly at a small amplitude for a predetermined time so as to cause the reaction containers to be vibrated arcuately each time a new reaction container is supplied with the reagent. As a result of the vibration of the reaction containers, the agitation balls in the respective reaction containers vigorously move to agitate the reaction solutions in the respective reaction containers. A photometric device is disposed so as to apply a light to the reaction container thereby to allow a fluorescent or absorbance photometric measurement. In the reaction container brought to the photometric measuring position, the agitation ball is attracted to the wall of the reaction container so as to be kept away from the path of light in the photometric device.

摘要翻译： 多个反应容器各自装有由磁性材料制成的搅拌球，循环地布置在反应盘上，以供给试剂和样品。 反应盘以小的振幅往复运动预定时间，以便每次向反应容器提供新的试剂时使反应容器发生弓形振动。 由于反应容器的振动，各反应容器中的搅拌球剧烈移动以搅拌各反应容器中的反应溶液。 设置光度测量装置，以便向反应容器施加光，从而进行荧光或吸光度光度测量。 在被送到测光测量位置的反应容器中，搅拌球被吸引到反应容器的壁上，以便远离测光装置中的光线。

公开(公告)号：US5104808A

公开(公告)日：1992-04-14

申请号：US237119

申请日：1988-08-26

申请人： Paul F. Laska ,  Kyoko Imai ,  Hiroshi Hashimoto ,  Hajime Betsui ,  Hiroshi Umetsu

发明人： Paul F. Laska ,  Kyoko Imai ,  Hiroshi Hashimoto ,  Hajime Betsui ,  Hiroshi Umetsu

IPC分类号： G01N33/543 ,  G01N35/00 ,  G01N35/02

CPC分类号： G01N35/00732 ,  G01N33/54366 ,  G01N35/0092 ,  G01N35/0098 ,  G01N35/025 ,  G01N2035/00435 ,  Y10T436/114165 ,  Y10T436/115831

摘要： A diagnostic instrument and method indexes plural, sequentially actuated reaction vessels, stepwise to several processing positions. Several positions effecting wash have wash probes that are gauged together. Sample and/or reagent are not added to a number of vessels leading and trailing sample containing vessels. The processing of the sample is controlled according to different time-templates and the first use of the wash probes occurs in the same cycle for different sets of analytical tests.

公开(公告)号：US5051238A

公开(公告)日：1991-09-24

申请号：US272345

申请日：1988-11-17

申请人： Hiroshi Umetsu ,  Hiroshi Hashimoto ,  Kyoko Imai ,  Hajime Betsui ,  Tadashi Ohishi

发明人： Hiroshi Umetsu ,  Hiroshi Hashimoto ,  Kyoko Imai ,  Hajime Betsui ,  Tadashi Ohishi

IPC分类号： G01N1/00 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04

CPC分类号： G01N35/025 ,  G01N2035/00386 ,  G01N2035/00435 ,  G01N2035/0448 ,  G01N35/0098 ,  G01N35/1079

摘要： An automatic analyzing system has a sample table for supporting a plurality of sample vessels and a reagent table for supporting a plurality of reagent vessels. The sample table and the reagent table are fixed to a common drive shaft. The system further has a rotatable reaction table for supporting a plurality of reaction vessels. The reaction table is arranged in a side-by-side relation to the sample table and the reagent table. The system further has a single pipetting device disposed between the reaction table and the stack of the sample table and the reagent table for a swinging motion therebetween so as to suck samples and reagents from the sample vessels and the reagent vessels and to deliver the same to the reaction vessels. The pipetting device is operated in such a manner as to transfer all the samples to the reaction vessels followed by the supply of the reagents to these reaction vessels.

摘要翻译： 自动分析系统具有用于支撑多个样本容器的样品台和用于支撑多个试剂容器的试剂台。 样品台和试剂台固定在共同的驱动轴上。 该系统还具有用于支撑多个反应容器的可旋转反应台。 反应台与样品台和试剂台并列排列。 该系统还具有设置在反应台和样品台和试剂台之间的单个移液装置，用于在样品台和试剂台之间摆动，从样品容器和试剂容器吸取样品和试剂，并将其送至 反应容器。 移液装置以将所有样品转移到反应容器，然后向这些反应容器供应试剂的方式操作。

公开(公告)号：US5882863A

公开(公告)日：1999-03-16

申请号：US658398

申请日：1996-06-05

申请人： Kyoko Imai ,  Junko Momose ,  Yasushi Nomura

发明人： Kyoko Imai ,  Junko Momose ,  Yasushi Nomura

IPC分类号： C12Q1/68 ,  G01N33/50 ,  G01N33/58 ,  C12Q1/70 ,  C07H21/02 ,  G01N33/544

CPC分类号： C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/683

摘要： A nucleic acid sample is mixed with a nucleotide reagent under hybridizing conditions. The nucleotide reagent contains nucleotides each combined at 3'- and 5'-terminals thereof with particles. After cleaving double strands of hybridization products, at least ones of particles separated from each other by the cleaving and particles held in the unreacted reagent and subjected to no cleavage are detected for measuring nucleic acids in the sample. Since the particles separated from each other by the cleaving and the particles held in the unreacted reagent and subjected to no cleavage are different in size of particle masses from each other, these two groups of particles can be discriminated and detected. Therefore, a labeling substance in the unreacted reagent is no longer required to be washed and separated, enabling nucleic acids to be quickly measured with high sensitivity.

摘要翻译： 在杂交条件下将核酸样品与核苷酸试剂混合。 核苷酸试剂含有在3'和5'末端与颗粒结合的核苷酸。 在切割双链杂交产物之后，检测至少一个通过切割彼此分离的颗粒和保持在未反应试剂中的颗粒并且不进行切割，以测量样品中的核酸。 由于通过裂开彼此分离的颗粒和保持在未反应试剂中的颗粒彼此不分裂，所以可以鉴别和检测这两组颗粒。 因此，不再需要清洗和分离未反应试剂中的标记物质，能够以高灵敏度快速测定核酸。

公开(公告)号：US5746977A

公开(公告)日：1998-05-05

申请号：US714883

申请日：1996-09-13

申请人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

发明人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

IPC分类号： G01N35/00 ,  G05B19/042 ,  G01N37/00

CPC分类号： G01N35/00594 ,  G05B19/0428 ,  G01N2035/009 ,  G01N35/00871 ,  Y10T436/115831 ,  Y10T436/12

摘要： An automatic analyzer in which a suitable transmission destination remote from a body of the automatic analyzer is selected in accordance with the contents of to be transmitted and the contents are transmitted to the transmission destination; a suitable operation control instruction is received from a suitable person on duty; and a suitable process is carried out to make it possible to improve analysis efficiency.

摘要翻译： 一种自动分析器，其中根据要发送的内容选择远离自动分析仪的主体的适当的发送目的地，并将内容发送到发送目的地; 由合适的人员接收适当的操作控制指令; 并且进行适当的处​​理以使得可以提高分析效率。

公开(公告)号：US5128241A

公开(公告)日：1992-07-07

申请号：US140117

申请日：1987-12-31

申请人： Kyoko Imai ,  Yasushi Nomura

发明人： Kyoko Imai ,  Yasushi Nomura

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/544 ,  G01N33/74

CPC分类号： G01N33/5432 ,  G01N33/74 ,  Y10S435/966 ,  Y10S435/975 ,  Y10S436/821 ,  Y10S436/829

摘要： Microcapsule-reagents are prepared by previously reacting at least a part of an antigen or antibody attached to microcapsules encapsulating a labeling substance with an antibody or antigen which is specifically reactive therewith, and then the reagent thus prepared is reacted with a sample containing an antigen or antibody in the presence of a complement, whereby highly sensitive immunoassay of the antigen or antibody in the sample can be realized even when the antigen or antibody attached to the microcapsules has a lowered activity or has only low reactivity with the antibody or antigen in the sample.

公开(公告)号：US5055415A

公开(公告)日：1991-10-08

申请号：US177347

申请日：1988-04-01

申请人： Kyoko Imai ,  Yasushi Nomura

发明人： Kyoko Imai ,  Yasushi Nomura

IPC分类号： G01N27/26 ,  G01N27/447 ,  G01N33/53 ,  G01N33/531 ,  G01N33/561 ,  G01N33/74 ,  G01N33/78

CPC分类号： G01N33/53 ,  G01N33/531 ,  G01N33/561 ,  G01N33/743 ,  G01N33/78 ,  Y10S436/806 ,  Y10S436/819

摘要： A method for immunoassay for an antigen involves the following steps: reacting a sample possibly containing an antigen to be determined, a definite amount of a conjugate of the antigen and a charged substance and a definite amount of labeled antibody specific for antigen; allowing the reaction products to electrophoretically migrate towards a carrier bearing an attached antibody specific for the charged substance, so that when the reaction products contact the carrier any reaction product of labeled antibody and antigen passes through the carrier and any reaction product of the labeled antibody and conjugate binds to the carrier attached antibody specific for the charged substance; determining the amount of labeled antibody thereby bound to the carrier and relating the determined amount of labeled antibody to the amount of antigen in the sample.

摘要翻译： 用于抗原的免疫测定方法包括以下步骤：使可能含有待测抗原的样品，确定量的抗原与带电物质的缀合物和一定量的抗原特异性标记抗体反应; 允许反应产物电泳迁移到携带具有针对带电物质的附着抗体的载体，使得当反应产物与载体接触时，标记的抗体和抗原的任何反应产物通过载体和标记抗体的任何反应产物和 缀合物结合对于带电物质特异性的载体附着的抗体; 确定由此结合到载体上的标记抗体的量，并将确定的标记抗体量与样品中的抗原量相关联。

公开(公告)号：US5543292A

公开(公告)日：1996-08-06

申请号：US76165

申请日：1993-06-14

申请人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

发明人： Kyoko Imai ,  Kazumichi Imai ,  Yasushi Nomura

IPC分类号： C12Q1/68 ,  G01N15/14 ,  G01N35/00 ,  G01N35/02

CPC分类号： G01N35/025 ,  C12Q1/6823 ,  C12Q1/6834 ,  G01N2015/1409 ,  G01N2035/00485

摘要： A process for detecting the existence of at least one sequence of oligonucleotide in a nucleic acid sample includes at least one performance of a step of mixing the sample witha labeled common polynucleotide, anda polynucleotide probe comprising a sequence complementary to at least a part of the sequence of oligonucleotide and a sequence complementary to at least a part of the labeled common polynucleotide. The existence of a sequence of oligonucleotide which is an analyte existing in a nucleic acid sample can be detected by the use of a common reagent and an unattached polynucleotide having a base sequence specific for the analyte to be measured.

摘要翻译： 用于检测核酸样品中至少一个寡核苷酸序列的存在的方法包括将样品与标记的共同多核苷酸混合的步骤的至少一个性能，以及包含与至少一部分 寡核苷酸序列和与标记的共同多核苷酸的至少一部分互补的序列。 存在核酸样品中存在的分析物的寡核苷酸序列的存在可以通过使用具有对待测量的分析物特异的碱基序列的常见试剂和未附着的多核苷酸来检测。

公开(公告)号：US5770461A

公开(公告)日：1998-06-23

申请号：US520781

申请日：1995-08-30

申请人： Taku Sakazume ,  Kyoko Imai ,  Yasushi Nomura

发明人： Taku Sakazume ,  Kyoko Imai ,  Yasushi Nomura

IPC分类号： B03C1/28 ,  G01N33/543 ,  G01N33/553

CPC分类号： B03C1/288 ,  G01N33/54326 ,  Y10S436/806 ,  Y10S436/824

摘要： A method and apparatus for separating solid supports formed of magnetic particles on which immunocomplexes are bound, from a liquid phase includes a reaction container containing a fluid in which magnetic particles having immunocomplex bound thereon are suspended. The reaction container is positioned at a flock forming station, where the fluid is subjected to an alternate current magnetic field having a temporally varying intensity by an electromagnetic. The magnetic particles are flocculated with each other due to the application of such varying magnetic field to form a multiplicity of flocks. A stronger magnetic field then is applied to the resulting contents in the reaction container by a permanent magnet. The flocks are magnetically fixed on the inner wall surface of the reaction container by the action of the stronger magnetic field. While the magnetic particles are being fixed on the inner wall surface, a nozzle is inserted into the reaction container to suck the unnecessary liquid phase and remove it from the reaction container.

摘要翻译： 用于从液相中分离形成有免疫复合物的磁性颗粒形成的固体载体的方法和装置包括含有其中悬浮有结合有免疫复合物的磁性颗粒的流体的反应容器。 反应容器定位在成形站处，其中流体经受电磁时具有时间上变化的强度的交流磁场。 磁性颗粒由于施加这种变化的磁场而相互絮凝以形成多个绒毛。 然后通过永磁体将更强的磁场施加到反应容器中的所得内容物。 羊群通过较强的磁场的作用被磁性地固定在反应容器的内壁表面上。 当磁性颗粒固定在内壁表面上时，将喷嘴插入反应容器中以吸收不需要的液相并将其从反应容器中取出。