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    SYSTEM FOR ANALYZING SINGLE CELL
    2.
    发明申请
    SYSTEM FOR ANALYZING SINGLE CELL 审中-公开

    公开(公告)号：US20190194645A1

    公开(公告)日：2019-06-27

    申请号：US16329682

    申请日：2017-03-06

    申请人： Hitachi, Ltd.

    发明人： Tomoyuki SAKAI ,  Masataka SHIRAI

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1013 ,  C12M1/00 ,  C12M1/34 ,  C12N15/09

    摘要： A device is provided for capturing a nucleic acid in a single cell, having: a two-dimensional array comprising a substrate, a plurality of single cell capturing holes provided on one surface of the substrate, and a nucleic acid capturing region comprising, on the inside of the substrate, a nucleic acid capturing body, which is configured to capture the nucleic acids extracted from the individual cells respectively captured by the single cell capturing hole; a flow channel which is provided adjacent to the nucleic acid capturing region of the substrate, and is configured to discharge a solution in the nucleic acid capturing region; and a cylindrical structure body which is arranged on the substrate at the time of introducing a cell suspension and encloses a plurality of the single cell capturing holes, wherein the cylindrical structure body is removed after the cells are captured by the single cell capturing holes.

    Two-Dimensional Cell Array Device and Apparatus for Gene Quantification and Sequence Analysis
    3.
    发明申请
    Two-Dimensional Cell Array Device and Apparatus for Gene Quantification and Sequence Analysis 审中-公开
    用于基因定量和序列分析的二维细胞阵列装置和装置

    公开(公告)号：US20160010078A1

    公开(公告)日：2016-01-14

    申请号：US14771339

    申请日：2013-03-12

    申请人： HITACHI, LTD.

    发明人： Masataka SHIRAI ,  Hideki KAMBARA ,  Kiyomi TANIGUCHI ,  Maiko TANABE

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1003 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/1805 ,  B01L2400/0421 ,  C12N15/1096 ,  C12Q1/6874 ,  C12Q2539/10

    摘要： In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.

    摘要翻译： 为了对许多细胞中的许多基因进行基因表达分析，必须分离细胞，从中提取基因，扩增核酸并进行序列分析。 然而，细胞的分离对细胞造成损害，并且需要使用昂贵的系统。 通过在垂直方向布置包含细胞捕获部分和核酸捕获部分的一对结构以提取相关细胞中的单个基因，可以高精度地进行每个细胞中的基因表达分析，在核酸捕获部分中合成cDNA ，扩增核酸，并使用下一代测序仪分析序列。

    GENE ANALYSIS SYSTEM
    5.
    发明申请
    GENE ANALYSIS SYSTEM 审中-公开

    公开(公告)号：US20180119135A1

    公开(公告)日：2018-05-03

    申请号：US15553175

    申请日：2015-04-09

    申请人： Hitachi, Ltd.

    发明人： Maiko TANABE ,  Masataka SHIRAI ,  Tomoyuki SAKAI

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1065 ,  C12M1/00 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Q2563/179

    摘要： In order to interpret an arbitrary sequence region in many genes in many cells, it is necessary to degrade a nucleic acid into fragments and introduce a sequence that is different from one cell to another into each of the fragments. However, in the conventional configuration for analyzing many cells, there has been a problem that mixing of the degraded fragments among areas occurs before a tag sequence unique for each of the areas is introduced. The present invention provides a system for capturing a nucleic acid extracted from a cell in each of plural areas on a substrate and synthesizing a complementary DNA (cDNA) of the nucleic acid for each of the areas, wherein the system also includes a means for immediately introducing a tag sequence unique for each of the areas to the reaction product.

    DATA ANALYSIS APPARATUS AND DATA ANALYSIS METHOD
    6.
    发明申请
    DATA ANALYSIS APPARATUS AND DATA ANALYSIS METHOD 审中-公开
    数据分析装置和数据分析方法

    公开(公告)号：US20150302042A1

    公开(公告)日：2015-10-22

    申请号：US14443118

    申请日：2012-11-20

    申请人： HITACHI, LTD.

    发明人： Masataka SHIRAI ,  Hideki KAMBARA ,  Kiyomi TANIGUCHI

    IPC分类号： G06F17/30 ,  G06F19/28

    CPC分类号： G06F16/2365 ,  G06F16/285 ,  G16B40/00 ,  G16B50/00

    摘要： The present invention aims to provide a data analysis apparatus capable of clustering appropriately even when there is an exceptional datum resulted from an experimental error and the like. In the data analysis apparatus according to the invention, a cluster range parameter for stretching a cluster boundary is determined in advance according to the range of an experimental error which an experimental error datum describes. In the process of clustering, an exceptional datum which does not belong to any cluster is determined to belong to a cluster when an area at a distance determined by the cluster range parameter from the exceptional datum is contained in the cluster, and the exceptional datum is determined to form an independent cluster when even the area at the distance is not contained in any cluster (see FIG. 7).

    摘要翻译： 本发明的目的在于提供即使在存在由实验误差等引起的异常原理的情况下也能适当聚类的数据分析装置。 在根据本发明的数据分析装置中，根据实验误差基准描述的实验误差的范围，预先确定用于拉伸簇边界的簇范围参数。 在聚类过程中，当集群中包含由特殊基准的聚类范围参数确定的距离范围内的区域被确定为属于集群时，不属于任何集群的特殊数据，并且异常数据为 确定为即使在距离上的区域不包含在任何集群中时形成独立的集群（参见图7）。

    SYSTEM FOR CAPTURING SINGLE CELL-DERIVED BIOMOLECULES
    8.
    发明申请
    SYSTEM FOR CAPTURING SINGLE CELL-DERIVED BIOMOLECULES 审中-公开

    公开(公告)号：US20180272349A1

    公开(公告)日：2018-09-27

    申请号：US15762274

    申请日：2016-03-28

    申请人： Hitachi, Ltd.

    发明人： Tomoyuki SAKAI ,  Masataka SHIRAI

    IPC分类号： B01L3/00 ,  C12N15/10

    CPC分类号： B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0893 ,  B01L2400/086 ,  C12M1/34 ,  C12N15/1013

    摘要： A system for capturing a biomolecule in a single cell includes: a two-dimensional array having single cell capture holes on one surface of a substrate, and biomolecule capture areas inside the substrate each comprising a biomolecule capture member for capturing a biomolecule extracted from individual cells respectively captured by the single cell capture holes; a flow channel for flowing a sample containing cells to be assayed, from a 1st direction parallel to the surface of the substrate; a structure on the surface of the substrate and opposed to the 1st direction on the downstream side of each of the single cell capture holes; a 1st application means applying a 1st flow, and a 1st control means; and a 2nd application means applying a 2nd flow orthogonal to the one surface of the substrate toward each biomolecule capture area from each corresponding single cell capture hole, and a 2nd control means.