公开(公告)号：US10343165B2

公开(公告)日：2019-07-09

申请号：US15396175

申请日：2016-12-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amir Sadri ,  Kun Guo ,  Daniel Y. Chu ,  Nenad Kircanski ,  Paul J. Patt ,  Tal Rosenzweig

IPC: G01N1/18 ,  G01N1/00 ,  B01D21/00 ,  B01L3/00 ,  G01N15/14 ,  B01L3/02 ,  C12Q1/24 ,  G01N15/10

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

公开(公告)号：US20130270287A1

公开(公告)日：2013-10-17

申请号：US13861143

申请日：2013-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kun Guo ,  Paul J. Patt

IPC: G06F17/00

CPC classification number: G06F17/00 ,  B01L3/0289 ,  B01L2200/0652 ,  B01L2200/0657 ,  B01L2200/143 ,  B01L2400/0439 ,  B01L2400/0478 ,  B01L2400/0481 ,  G01N15/02 ,  G01N15/0205 ,  G01N2015/1062 ,  G01N2015/1486

Abstract: Particles are dispensed under controlled conditions to achieve an accurate number of particles by passing a suspension of the particles through a particle detecting device, obtaining a cumulative particle count, comparing the cumulative particle count with a target value, and shutting off the suspension flow once the particle count reaches the target value, all performed by automated means.

Abstract translation: 在受控条件下分配颗粒以通过使颗粒的悬浮液通过颗粒检测装置获得精确数量的颗粒，获得累积颗粒数，将累积颗粒数与目标值进行比较，并一旦关闭悬浮液流 粒子数达到目标值，全部以自动方式进行。

公开(公告)号：US20170128941A1

公开(公告)日：2017-05-11

申请号：US15396175

申请日：2016-12-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amir Sadri ,  Kun Guo ,  Daniel Y. Chu ,  Nenad Kircanski ,  Paul J. Patt ,  Tal Rosenzweig

IPC: B01L3/00 ,  G01N15/14

CPC classification number: B01L3/502761 ,  B01L3/0265 ,  B01L3/502738 ,  B01L3/502776 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/16 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0655 ,  B01L2400/0688 ,  B01L2400/082 ,  B01L2400/084 ,  C12Q1/24 ,  G01N15/1056 ,  G01N15/1404 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/1413 ,  G01N2015/149 ,  Y10T436/25 ,  Y10T436/25375

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

公开(公告)号：US20170241888A1

公开(公告)日：2017-08-24

申请号：US15503369

申请日：2015-08-19

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Daniel Y. Chu ,  Paul J. Patt ,  Roger Tong

IPC: G01N15/14 ,  C12Q1/04 ,  B01L3/00

CPC classification number: G01N15/1404 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0424 ,  B01L2400/0454 ,  C12Q1/04 ,  G01N2015/1006 ,  G01N2015/1415

Abstract: A method is provided for transporting a plurality of cells through a flow chamber, wherein the cells are initially immobilized on an internal surface of the flow chamber. The method comprises: selectively releasing the cells from the internal surface of the flow chamber; and flowing liquid through the flow chamber such that the released cells travel with the liquid, thereby transporting the cells through the flow chamber. Cells can be immobilized on or selectively released from the internal surface by applying or removing a trapping potential. The trapping potential can arise from an electric field gradient or an optical field gradient. Alternatively, cells can be selectively released from the surface using photocatalysis. Selective release allows cells to be individually observed or analyzed downstream, and can be based on a signal detected from one or more cells immobilized on the surface.

公开(公告)号：US10466156B2

公开(公告)日：2019-11-05

申请号：US15503369

申请日：2015-08-19

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Daniel Y. Chu ,  Paul J. Patt ,  Roger Tong

IPC: G01N15/14 ,  B01L3/00 ,  C12Q1/04 ,  G01N15/10

Abstract: A method is provided for transporting a plurality of cells through a flow chamber, wherein the cells are initially immobilized on an internal surface of the flow chamber. The method comprises: selectively releasing the cells from the internal surface of the flow chamber; and flowing liquid through the flow chamber such that the released cells travel with the liquid, thereby transporting the cells through the flow chamber. Cells can be immobilized on or selectively released from the internal surface by applying or removing a trapping potential. The trapping potential can arise from an electric field gradient or an optical field gradient. Alternatively, cells can be selectively released from the surface using photocatalysis. Selective release allows cells to be individually observed or analyzed downstream, and can be based on a signal detected from one or more cells immobilized on the surface.

公开(公告)号：US09535000B2

公开(公告)日：2017-01-03

申请号：US14479132

申请日：2014-09-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kun Guo ,  Amir M. Sadri ,  Daniel Y. Chu ,  Nenad Kircanski ,  Paul J. Patt ,  Tal Rosenzweig

IPC: G01N33/00 ,  G01N1/18 ,  B01L3/00 ,  F04B19/00 ,  G01N15/14 ,  B01L3/02 ,  C12Q1/24

CPC classification number: G01N15/1459 ,  B01L3/0265 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0655 ,  B01L2400/0688 ,  B01L2400/082 ,  B01L2400/084 ,  C12Q1/24 ,  G01N15/1404 ,  G01N2015/1413 ,  G01N2015/149 ,  Y10T436/25 ,  Y10T436/25375

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

Abstract translation: 用于微流体操纵，分配和/或分选颗粒例如细胞和/或珠粒的系统，包括装置和方法。