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公开(公告)号：US20180291337A1
公开(公告)日：2018-10-11
申请号：US15785029
申请日：2017-10-16
IPC分类号： C12N5/0735
CPC分类号： A61K35/12 , C12N5/0606 , C12N15/1034 , C12N15/1072 , C12N15/1096 , C12N2500/14 , C12N2500/30 , C12N2500/38 , C12N2500/44 , C12N2500/62 , C12N2501/105 , C12N2501/13 , C12N2501/155 , C12N2501/385 , C12N2501/39 , C12N2501/999 , C12N2502/13 , C12N2502/99 , C12N2503/02 , C12N2510/00 , C12N2510/04 , C12N2533/90
摘要： This invention provides a system for efficiently producing differentiated cells from pluripotent cells, such as human embryonic stem cells. Rather than permitting the cells to form embryoid bodies according to established techniques, differentiation is effected directly in monolayer culture on a suitable solid surface. The cells are either plated directly onto a differentiation-promoting surface, or grown initially on the solid surface in the absence of feeder cells and then exchanged into a medium that assists in the differentiation process. The solid surface and the culture medium can be chosen to direct differentiation down a particular pathway, generating a cell population that is remarkably uniform. The methodology is well adapted to bulk production of committed precursor and terminally differentiated cells for use in drug screening or regenerative medicine.
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公开(公告)号：US20140242691A1
公开(公告)日：2014-08-28
申请号：US14217699
申请日：2014-03-18
发明人： Melissa K. Carpenter
IPC分类号： C12N5/079
CPC分类号： C12N5/0618 , A61K35/12 , C12N5/0623 , C12N2501/01 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/13 , C12N2501/135 , C12N2503/02 , C12N2506/02 , C12N2510/00 , C12Q1/6881 , C12Q2600/158
摘要： This invention provides populations of neural progenitor cells, differentiated neurons, glial cells, and astrocytes. The populations are obtained by culturing stem cell populations (such as embryonic stem cells) in a cocktail of growth conditions that initiates differentiation, and establishes the neural progenitor population. The progenitors can be further differentiated in culture into a variety of different neural phenotypes, including dopaminergic neurons. The differentiated cell populations or the neural progenitors can be generated in large quantities for use in drug screening and the treatment of neurological disorders.
摘要翻译： 本发明提供了神经祖细胞，分化神经元，神经胶质细胞和星形胶质细胞的群体。 通过在启动分化的生长条件的混合物中培养干细胞群（例如胚胎干细胞）获得种群，并建立神经祖细胞群体。 祖细胞可以进一步在培养中分化成各种不同的神经表型，包括多巴胺能神经元。 可以大量产生分化的细胞群或神经祖细胞用于药物筛选和治疗神经障碍。
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公开(公告)号：US20140106343A1
公开(公告)日：2014-04-17
申请号：US14010578
申请日：2013-08-27
发明人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
IPC分类号： C12N5/071
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2500/62 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/13 , C12N2501/148 , C12N2501/155 , C12N2501/23 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2502/13 , C12N2503/02 , C12N2506/02 , C12N2533/90 , C12N2533/92
摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。
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公开(公告)号：US09587223B2
公开(公告)日：2017-03-07
申请号：US14010578
申请日：2013-08-27
发明人： Ramkumar Mandalam , Saadia Faouzi , Isabelle Nadeau , Kristina Pfendler-Bonham , Namitha Rao , Melissa K. Carpenter , Lakshmi Rambhatla , Choy-Pik Chiu
IPC分类号： C12N5/071
CPC分类号： C12N5/067 , C12N2500/30 , C12N2500/36 , C12N2500/62 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/13 , C12N2501/148 , C12N2501/155 , C12N2501/23 , C12N2501/33 , C12N2501/335 , C12N2501/385 , C12N2501/39 , C12N2502/13 , C12N2503/02 , C12N2506/02 , C12N2533/90 , C12N2533/92
摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.
摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。
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公开(公告)号：US10351821B2
公开(公告)日：2019-07-16
申请号：US15785029
申请日：2017-10-16
IPC分类号： A61K35/12 , C12N5/0735 , C12N15/10
摘要： This invention provides a system for efficiently producing differentiated cells from pluripotent cells, such as human embryonic stem cells. Rather than permitting the cells to form embryoid bodies according to established techniques, differentiation is effected directly in monolayer culture on a suitable solid surface. The cells are either plated directly onto a differentiation-promoting surface, or grown initially on the solid surface in the absence of feeder cells and then exchanged into a medium that assists in the differentiation process. The solid surface and the culture medium can be chosen to direct differentiation down a particular pathway, generating a cell population that is remarkably uniform. The methodology is well adapted to bulk production of committed precursor and terminally differentiated cells for use in drug screening or regenerative medicine.
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