公开(公告)号：US09587223B2

公开(公告)日：2017-03-07

申请号：US14010578

申请日：2013-08-27

申请人： Asterias Biotherapeutics, Inc.

发明人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa K. Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

IPC分类号： C12N5/071

CPC分类号： C12N5/067 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/62 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/23 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2533/90 ,  C12N2533/92

摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.

摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。

公开(公告)号：US20140106343A1

公开(公告)日：2014-04-17

申请号：US14010578

申请日：2013-08-27

申请人： Asterias Biotherapeutics, Inc.

发明人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa K. Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

IPC分类号： C12N5/071

CPC分类号： C12N5/067 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/62 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/23 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2533/90 ,  C12N2533/92

摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.

摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。