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公开(公告)号：US20230139899A1
公开(公告)日：2023-05-04
申请号：US18074272
申请日：2022-12-02
IPC分类号： C12N5/0793
摘要： Methods for differentiating human pluripotent stem cells to dorsal neuroectoderm progenitors and further to glial progenitor cells and oligodendrocyte progenitor cells (OPCs) using inhibitors of BMP signaling and MAPK/ERK signaling are provided. Also provided are cells and cellular compositions obtained by such methods, and uses of such cells. Further provided are methods and protocols for efficiently differentiating human pluripotent stem cells to OPCs in the absence of the ventralizing morphogen SHH or a SHH signaling activator. The methods of the present disclosure reproducibly produce dorsal neuroectoderm progenitor cells by day 7 of the differentiation process, glial progenitor cells by day 21 of the differentiation process and OPCs by day 42 of the differentiation process.
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公开(公告)号：US20210189335A1
公开(公告)日：2021-06-24
申请号：US16090590
申请日：2017-03-30
发明人： Erik Michael WHITELEY , Uzma SHOUKAT-MUMTAZ , Rashi SRIVASTAVA , Nathan Charles MANLEY , Casey Christopher CASE
摘要： Compositions comprising a population of oligodendrocyte progenitor cells (OPCs), as well as methods of making and using the same, are provided. In one aspect, a container comprising a composition, where the composition comprises a population of cells comprising a plurality of OPCs, and where the population of cells comprises less than 15% undesirable cell types is provided. In another aspect, the population of cells comprises less than 15% undesirable epithelial lineage cells. In yet another aspect, the population of cells comprises less than 2% K7 positive cells. In an aspect, a population of cells comprising a plurality of oligodendrocyte progenitor cells is capable of forming less than one epithelial cyst per 100,000 cells in a cyst assay is provided. An even further aspect of the present disclosure is a container comprising a composition, where the composition comprising a plurality of oligodendrocyte progenitor cells is useful in treating treat stroke, spinal cord injury, and multiple sclerosis.
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3.发明申请公开(公告)号：US20190262405A1
公开(公告)日：2019-08-29
申请号：US16333566
申请日：2017-09-14
摘要： Methods and compositions for making and using pluripotent stem cell-derived oligodendrocyte progenitor cells for the treatment of spinal cord injury are disclosed.
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公开(公告)号：US10344262B2
公开(公告)日：2019-07-09
申请号：US15426030
申请日：2017-02-06
IPC分类号： C12N5/0784 , A61K39/00
摘要： The invention provides methods of differentiating primate pluripotent stem cells into cells of hematopoietic lineage. The invention further provides hematopoietic lineage cells differentiated from primate pluripotent stem cells, as well as methods of using the same and kits comprising the same.
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公开(公告)号：US10286009B2
公开(公告)日：2019-05-14
申请号：US15156316
申请日：2016-05-16
IPC分类号： A61K35/30 , A61K31/436 , A61K9/00 , C12N5/079
摘要： The present disclosure provides methods and compositions for making and using pluripotent stem cell-derived oligodendrocyte progenitor cells.
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6.发明授权公开(公告)号：US09074182B2
公开(公告)日：2015-07-07
申请号：US13679663
申请日：2012-11-16
IPC分类号： C12N5/074 , C12N5/0797
CPC分类号： C12N5/0622 , C12N5/0081 , C12N5/0607 , C12N5/0623 , C12N2502/09 , C12N2502/23 , C12N2506/02
摘要： The invention provides methods for depleting extraneous phenotypes from a mixed population of cells comprising the in vitro differentiated progeny of primate pluripotent stem cells. The invention also provides mixed cell populations enriched for a target cell phenotype where the mixed cell population comprises the differentiated in vitro progeny of primate embryonic stem cells.
摘要翻译： 本发明提供从包含灵长类动物多能干细胞的体外分化后代的混合细胞群体中消耗外来表型的方法。 本发明还提供了富集靶细胞表型的混合细胞群，其中混合细胞群体包含灵长类动物胚胎干细胞分化的体外后代。
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公开(公告)号：US20240082311A1
公开(公告)日：2024-03-14
申请号：US18262909
申请日：2022-01-28
发明人： Francois BINETTE , Jennifer BAHR-DAVIDSON , Rami SKALITER , Kento ONISHI , Nathan C. MANLEY , Craig R. HALBERSTADT , Erik M. WHITELEY
CPC分类号： A61K35/30 , A61K9/0019 , A61K9/0085 , A61P25/00 , C12N5/0622 , C12N2500/38 , C12N2501/155 , C12N2501/727 , C12N2506/02
摘要： Provided herein are methods, compositions of matter, and devices for treating neurological diseases and illnesses, including spinal cord injury.
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公开(公告)号：US11603518B2
公开(公告)日：2023-03-14
申请号：US16750975
申请日：2020-01-23
IPC分类号： C12N5/0793
摘要： Methods for differentiating human pluripotent stem cells to dorsal neuroectoderm progenitors and further to glial progenitor cells and oligodendrocyte progenitor cells (OPCs) using inhibitors of BMP signaling and MAPK/ERK signaling are provided. Also provided are cells and cellular compositions obtained by such methods, and uses of such cells. Further provided are methods and protocols for efficiently differentiating human pluripotent stem cells to OPCs in the absence of the ventralizing morphogen SHH or a SHH signaling activator. The methods of the present disclosure reproducibly produce dorsal neuroectoderm progenitor cells by day 7 of the differentiation process, glial progenitor cells by day 21 of the differentiation process and OPCs by day 42 of the differentiation process.
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公开(公告)号：US10676714B2
公开(公告)日：2020-06-09
申请号：US15894842
申请日：2018-02-12
IPC分类号： C12N5/0735
摘要： This disclosure provides an improved system for culturing human embryonic stem cells. The cells are cultured in suspension so as to maximize the production capacity of the culture environment. The new culture system of this invention allows for bulk proliferation of hES cells in a more cost-effective manner, which facilitates commercial production of important products for use in human therapy.
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10.发明申请公开(公告)号：US20190336538A1
公开(公告)日：2019-11-07
申请号：US16409760
申请日：2019-05-10
IPC分类号： A61K35/30 , A61K31/436 , C12N5/079 , A61K9/00
摘要： The present disclosure provides methods and compositions for making and using pluripotent stem cell-derived oligodendrocyte progenitor cells.
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