Apparatus and methods for simultaneous operation of miniaturized reactors
    3.
    发明申请
    Apparatus and methods for simultaneous operation of miniaturized reactors 失效
    用于小型化反应堆同时操作的装置和方法

    公开(公告)号:US20050089993A1

    公开(公告)日:2005-04-28

    申请号:US10816046

    申请日:2004-04-01

    摘要: The present invention provides a variety of microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. The invention further provides a chamber to contain the microfermentors and microfermentor arrays and to provide environmental control. Certain of the microfermentors include a second chamber that may be used, e.g., to provide oxygen, nutrients, pH control, etc., to the culture vessel and/or to remove metabolites, etc. Various methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided. The invention provides microreactors having a variety of different designs, some of which incorporate active stirring and/or have the capability to operate in batch or fed-batch mode. The invention further provides an apparatus and methods for simultaneous operation of a plurality of microreactors, with monitoring of the individual microreactors during a run. The invention further provides methods of performing gene expression analysis on cells cultured in microreactors.

    摘要翻译: 本发明提供用于培养细胞的各种微量生物反应器(微发酵器)和微生物生物反应器阵列。 微发酵器包括用于培养细胞的容器和用于以足以支持细胞生长(例如细菌细胞生长)的浓度向容器内部提供氧气的装置。 根据实施方案,微发酵罐容器可以具有小于约1ml的各种内部容积。 微发酵器可以包括曝气膜和任选的各种感测装置。 本发明进一步提供了容纳微发射体和微发光体阵列并提供环境控制的腔室。 某些微发酵器包括第二室,其可以用于例如向培养容器提供氧气,营养物质,pH控制等,和/或去除代谢物等。使用微发酵器的各种方法,例如, 选择最佳细胞株或生物过程参数。 本发明提供具有各种不同设计的微反应器,其中一些具有主动搅拌和/或具有以分批或分批模式操作的能力。 本发明还提供了一种用于同时操作多个微反应器的装置和方法,其中在运行期间监测各个微反应器。 本发明还提供了对在微反应器中培养的细胞进行基因表达分析的方法。

    Apparatus and methods for simultaneous operation of miniaturized reactors
    4.
    发明授权
    Apparatus and methods for simultaneous operation of miniaturized reactors 失效
    用于小型化反应堆同时操作的装置和方法

    公开(公告)号:US07507579B2

    公开(公告)日:2009-03-24

    申请号:US10816046

    申请日:2004-04-01

    摘要: The present invention provides a variety of microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. The invention further provides a chamber to contain the microfermentors and microfermentor arrays and to provide environmental control. Certain of the microfermentors include a second chamber that may be used, e.g., to provide oxygen, nutrients, pH control, etc., to the culture vessel and/or to remove metabolites, etc. Various methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided. The invention provides microreactors having a variety of different designs, some of which incorporate active stirring and/or have the capability to operate in batch or fed-batch mode. The invention further provides an apparatus and methods for simultaneous operation of a plurality of microreactors, with monitoring of the individual microreactors during a run. The invention further provides methods of performing gene expression analysis on cells cultured in microreactors.

    摘要翻译: 本发明提供用于培养细胞的各种微量生物反应器(微发酵器)和微生物生物反应器阵列。 微发酵器包括用于培养细胞的容器和用于以足以支持细胞生长(例如细菌细胞生长)的浓度向容器内部提供氧气的装置。 根据实施方案,微发酵罐容器可以具有小于约1ml的各种内部容积。 微发酵器可以包括曝气膜和任选的各种感测装置。 本发明进一步提供了容纳微发射体和微发光体阵列并提供环境控制的腔室。 某些微发酵器包括第二室,其可以用于例如向培养容器提供氧气,营养物质,pH控制等,和/或去除代谢物等。使用微发酵器的各种方法,例如, 选择最佳细胞株或生物过程参数。 本发明提供具有各种不同设计的微反应器,其中一些具有主动搅拌和/或具有以分批或分批模式操作的能力。 本发明还提供了一种用于同时操作多个微反应器的装置和方法,其中在运行期间监测各个微反应器。 本发明还提供了对在微反应器中培养的细胞进行基因表达分析的方法。

    Microbioreactor for continuous cell culture
    5.
    发明申请
    Microbioreactor for continuous cell culture 审中-公开
    用于连续细胞培养的微生物反应器

    公开(公告)号:US20060199260A1

    公开(公告)日:2006-09-07

    申请号:US11236453

    申请日:2005-09-26

    IPC分类号: C12M3/00 C12M1/12

    摘要: The present invention microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes of less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. Methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided. The microbioreactors having a variety of different designs, some of which incorporate active fluid mixing and/or have the capability to operate in batch, fed-batch, or continuous mode. In certain embodiments the microreactors operate as microchemostats. Methods for culturing cells under chemostat conditions in a microbioreactor are also provided.

    摘要翻译: 本发明的用于培养细胞的微生物生物反应器(微发酵器)和微生物生物反应器阵列。 微发酵器包括用于培养细胞的容器和用于以足以支持细胞生长(例如细菌细胞生长)的浓度向容器内部提供氧气的装置。 取决于实施方案,微发酵罐容器可以具有小于约1ml的各种内部体积。 微发酵器可以包括曝气膜和任选的各种感测装置。 提供了使用微发酵器的方法,例如选择最佳细胞株或生物过程参数。 具有各种不同设计的微生物反应器,其中一些结合了活性流体混合和/或具有以批量,补料分批或连续模式操作的能力。 在某些实施方案中,微反应器作为微量化学剂操作。 还提供了在微生物反应器中在恒化器条件下培养细胞的方法。