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公开(公告)号：US20240316480A1
公开(公告)日：2024-09-26
申请号：US18676015
申请日：2024-05-28
CPC分类号： B01D29/05 , B01D29/78 , B01D39/2027 , B01D39/2068 , C12M1/12 , C12M1/28 , B01D2201/0415
摘要： A filtration recovery method for filtering and recovering a liquid containing a filtering object that includes: sinking a filtration recovery device including a filtration filter having a plurality of through-holes, an inlet for introducing the liquid, an outlet for discharging the liquid, and a holder having a flow path communicating the inlet and the outlet, and the filtration filter is arranged in the flow path, into the liquid containing the filtering object contained in a liquid storage container with one end side of the holder provided with the outlet so as to capture the filtering object with the filtration filter; introducing the liquid from the outlet of the holder of the filtration recovery device; closing the outlet of the holder of the filtration recovery device; and recovering the filtering object together with the liquid held in the holder of the filtration recovery device.
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公开(公告)号：US20180051313A1
公开(公告)日：2018-02-22
申请号：US15556664
申请日：2016-03-15
CPC分类号： C12Q1/24 , B01D46/00 , C12M1/12 , C12M1/26 , C12M1/34 , C12Q1/04 , G01N1/10 , G01N1/405 , G01N2001/4088
摘要： Methods of detecting microorganisms in a fluid sample are provided. The method includes providing a nonwoven article, providing a fluid sample suspected of containing at least one microorganism strain or target cellular analyte, and contacting the fluid sample with the nonwoven article such that at least a portion of the at least one microorganism strain or target cellular analyte is bound to the nonwoven article. The nonwoven article includes a fibrous porous matrix and a concentration agent particles enmeshed in the fibrous porous matrix. The method further includes placing the microorganism strain- or target cellular analyte-bound nonwoven article in contact with at least one detection reagent and detecting the presence of the bound microorganism strain or bound target cellular analyte. Devices and kits containing the devices, for contacting a fluid sample with a nonwoven article, are also provided. The device includes a sample container, a filter holder, a nonwoven article, and an adaptor configured to interface the filter holder with a receptacle.
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公开(公告)号：US20140352382A1
公开(公告)日：2014-12-04
申请号：US14294829
申请日：2014-06-03
申请人： Evelyn N. WANG , Yangying ZHU , Rong XIAO
发明人： Evelyn N. WANG , Yangying ZHU , Rong XIAO
CPC分类号： B01L3/502761 , B01L3/502792 , B01L2200/0647 , B01L2300/0816 , B01L2300/123 , B01L2400/0484 , B23B3/10 , B23B27/20 , B41F5/24 , B41K3/62 , C12M1/12 , C12M1/42 , H01F1/0072 , H01L21/44 , H01L29/40 , Y10T428/24917
摘要： Provided in one embodiment is a dynamically tunable structure including an elastic layer, and a plurality of ferromagnetic micropillars disposed over the elastic layer. The elastic layer may have an elasticity that is greater than an elasticity of the micropillars.
摘要翻译： 在一个实施例中提供了一种动态可调谐结构，其包括弹性层和设置在弹性层上方的多个铁磁微柱。 弹性层可以具有大于微柱的弹性的弹性。
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公开(公告)号：US08642321B2
公开(公告)日：2014-02-04
申请号：US12082712
申请日：2008-04-14
申请人： Stephane Olivier , Mathieu Arrault
发明人： Stephane Olivier , Mathieu Arrault
摘要： The assembly comprises a filtration unit and a gel growth medium cassette, the unit and the cassette being adapted to cooperate in order to occupy a nested position in which a membrane covers the gel growth medium, the unit and the cassette each being equipped with an intermediate stop, it being possible for at least one of the two stops to move clear so as to allow the unit to move from an intermediate position, in which the membrane is spaced away from the gel growth medium, into the nested position. The method comprises selecting such an assembly, of engaging the unit on the cassette until the intermediate stop of the unit comes into contact with the intermediate stop of the cassette, and the step of moving clear the stop able to move clear so as to allow the unit to move from the intermediate position into the nested position.
摘要翻译： 所述组件包括过滤单元和凝胶生长培养基盒，所述单元和所述盒适于协作以便占据其中膜覆盖所述凝胶生长培养基的嵌套位置，所述单元和所述盒各配有中间体 停止，可以使两个止挡件中的至少一个移动清除，以便允许单元从隔膜与凝胶生长介质间隔开的中间位置移动到嵌套位置。 该方法包括：选择这样的组件，使单元与盒上的单元接合，直到单元的中间停止与盒的中间止挡接触，并且移动清除能够移动清除的停止的步骤，以便允许 单位从中间位置移动到嵌套位置。
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公开(公告)号：US20130316396A1
公开(公告)日：2013-11-28
申请号：US13984937
申请日：2012-02-22
申请人： Patric Fricking
发明人： Patric Fricking
IPC分类号： C12M1/26
摘要： The present invention relates to a bioreactor and a method for operating a bioreactor which comprises a chamber (100) capable of receiving a liquid media, and a filter assembly (200) comprising a perfusion filter (210; 212; 214). The filter assembly (200) is disposed in the chamber (100) and the filter assembly (200) is free to move within the chamber (100). The filter assembly (200) comprises means for coupling a harvesting flow through said filter (210; 212; 214) and means for coupling a feed flow through said filter in an opposite direction compared to the harvesting flow. Through this arrangement, the clogging prevention of the perfusion filter can be improved.
摘要翻译： 本发明涉及一种生物反应器和一种用于操作生物反应器的方法，该生物反应器包括能够接收液体介质的室（100）和包括灌注过滤器（210; 212; 214）的过滤器组件（200）。 过滤器组件（200）设置在室（100）中，并且过滤器组件（200）在室（100）内自由移动。 过滤器组件（200）包括用于将通过所述过滤器（210; 212; 214）的收集流耦合的装置和用于将进料流与收获流相反的方向与相反方向耦合通过所述过滤器的装置。 通过这种布置，可以提高灌注过滤器的堵塞防止。
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6.发明申请Methods and Devices for Multi-Dimensional Separation, Isolation and Characterization of Circulating Tumour Cells 审中-公开循环肿瘤细胞的多维分离，分离和表征的方法和装置公开(公告)号：US20130255361A1
公开(公告)日：2013-10-03
申请号：US13850596
申请日：2013-03-26
发明人： David Juncker , Nicholas Bertos
CPC分类号： C12M1/12 , B01L3/502707 , B01L3/502753 , B01L2300/0681 , B01L2300/087 , B01L2300/0874 , C12M47/04 , G01N1/10 , G01N15/0625 , G01N15/0656 , G01N2001/4088 , G01N2015/0065
摘要： In most cancers, a significant factor in a poor outcome for the individual cancer victim is metastatic disease, i.e., dissemination of tumour cells to other parts of the human body via the circulation, such as distant organs, and their subsequent proliferation therein to form multiple other cancer tumours. The presence of circulating tumour cells, or CTCs, represents a vital intermediate step in this process and variations of a few CTCs within blood samples containing tens of billions of cells may denote the outcome for a patient or impact the cancer treatment regimen. At present no low cost field deployable technique for filtering CTCs exists. According to embodiments of the invention micro-machined filters with high aspect ratio, with and without, functionalization are employed to perform multi-parameter filtering for CTCs based upon compatibility with low cost semiconductor processes within multiple materials including silicon, polymers and silicon carbide.
摘要翻译： 在大多数癌症中，个体癌症受害者的不良结果的重要因素是转移性疾病，即通过循环（例如远处器官）将肿瘤细胞传播到人体的其他部分，并且随后在其中增殖以形成多个 其他癌症肿瘤。 循环肿瘤细胞或CTCs的存在代表了该过程中重要的中间步骤，含有数百亿细胞的血液样本中几个CTC的变化可能表示患者的结果或影响癌症治疗方案。 目前，没有低成本现场部署用于过滤CTC的技术。 根据本发明的实施例，采用具有高纵横比，有无功能的微加工滤波器，以便基于与包括硅，聚合物和碳化硅在内的多种材料内的低成本半导体工艺的兼容性来执行CTC的多参数滤波。
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7.发明申请公开(公告)号：US20050089993A1
公开(公告)日：2005-04-28
申请号：US10816046
申请日：2004-04-01
申请人： Paolo Boccazzi , Angela Chen , Klavs Jensen , Nicolas Szita , Andrea Zanzotto , Zhiyu Zhang
发明人： Paolo Boccazzi , Angela Chen , Klavs Jensen , Nicolas Szita , Andrea Zanzotto , Zhiyu Zhang
CPC分类号： C12M1/12 , B01F13/0059 , B01F13/0827 , B01J2219/00283 , B01J2219/00317 , B01J2219/00479 , B01J2219/00481 , B01J2219/00484 , B01J2219/00495 , B01J2219/00585 , B01J2219/00599 , B01J2219/00691 , B01J2219/00743 , B01L3/5027 , B01L3/502715 , B01L3/502723 , B01L9/527 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/0877 , B01L2300/10 , B01L2400/0466 , B01L2400/0487 , C12M23/16 , C12M35/08 , C12M41/48 , G01N2035/00326 , Y10S435/808 , Y10S435/809
摘要： The present invention provides a variety of microscale bioreactors (microfermentors) and microscale bioreactor arrays for use in culturing cells. The microfermentors include a vessel for culturing cells and means for providing oxygen to the interior of the vessel at a concentration sufficient to support cell growth, e.g., growth of bacterial cells. Depending on the embodiment, the microfermentor vessel may have various interior volumes less than approximately 1 ml. The microfermentors may include an aeration membrane and optionally a variety of sensing devices. The invention further provides a chamber to contain the microfermentors and microfermentor arrays and to provide environmental control. Certain of the microfermentors include a second chamber that may be used, e.g., to provide oxygen, nutrients, pH control, etc., to the culture vessel and/or to remove metabolites, etc. Various methods of using the microfermentors, e.g., to select optimum cell strains or bioprocess parameters are provided. The invention provides microreactors having a variety of different designs, some of which incorporate active stirring and/or have the capability to operate in batch or fed-batch mode. The invention further provides an apparatus and methods for simultaneous operation of a plurality of microreactors, with monitoring of the individual microreactors during a run. The invention further provides methods of performing gene expression analysis on cells cultured in microreactors.
摘要翻译： 本发明提供用于培养细胞的各种微量生物反应器（微发酵器）和微生物生物反应器阵列。 微发酵器包括用于培养细胞的容器和用于以足以支持细胞生长（例如细菌细胞生长）的浓度向容器内部提供氧气的装置。 根据实施方案，微发酵罐容器可以具有小于约1ml的各种内部容积。 微发酵器可以包括曝气膜和任选的各种感测装置。 本发明进一步提供了容纳微发射体和微发光体阵列并提供环境控制的腔室。 某些微发酵器包括第二室，其可以用于例如向培养容器提供氧气，营养物质，pH控制等，和/或去除代谢物等。使用微发酵器的各种方法，例如， 选择最佳细胞株或生物过程参数。 本发明提供具有各种不同设计的微反应器，其中一些具有主动搅拌和/或具有以分批或分批模式操作的能力。 本发明还提供了一种用于同时操作多个微反应器的装置和方法，其中在运行期间监测各个微反应器。 本发明还提供了对在微反应器中培养的细胞进行基因表达分析的方法。
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公开(公告)号：US12030002B2
公开(公告)日：2024-07-09
申请号：US17095419
申请日：2020-11-11
CPC分类号： B01D29/05 , B01D29/78 , B01D39/2027 , B01D39/2068 , C12M1/12 , C12M1/28 , B01D2201/0415
摘要： A filtration recovery device according to the present disclosure may include: a filtration filter having a plurality of through-holes; and a holder having an introduction port configured to introduce a liquid, a discharge port configured to discharge the liquid, and a flow path configured to communicate the introduction port and the discharge port with each other, in which the filtration filter is disposed in the flow path between the introduction port and the discharge port of the holder.
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公开(公告)号：US11939621B2
公开(公告)日：2024-03-26
申请号：US17468617
申请日：2021-09-07
摘要： Provided is a device for determining the live/dead bacterial state, with which it is possible to ascertain the accurate state of live bacteria/dead bacteria via a captured image in a relatively easy operation with which ascertaining the state of a bacterial cell is more accurate than conventional methods. This device for determining the live/dead bacterial state comprises: a case body in which a measurement mechanism is housed; an opening/closing lid body that allows a fungus base insertion port formed in the case body to be opened and closed; and the measurement mechanism, which is housed in the case body and is configured to measure the number of bacteria. The measurement mechanism comprises: a fungus base holding mechanism that inserts and fixes a fungus base on which a bacterial cell collected from a specimen is placed; an excitation light irradiating mechanism configured so as to be capable of focusing and irradiating an excitation light toward the bacterial cell on the fungus base; an imaging camera disposed above the bacterial cell on the fungus base; and an XY-axes adjustment mechanism that minutely adjusts and moves, in the XY-axes, an XY stage supporting the fungus base holding mechanism and comprising two separately moving layers.
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公开(公告)号：US20240042102A1
公开(公告)日：2024-02-08
申请号：US18347151
申请日：2023-07-05
IPC分类号： A61L27/52 , B33Y10/00 , B33Y70/00 , B33Y80/00 , B29C64/112 , B29C64/245 , A61L27/56 , B29C64/00 , B29C35/16 , C12M1/00 , C12M1/12
CPC分类号： A61L27/52 , B33Y10/00 , B33Y70/00 , B33Y80/00 , B29C64/112 , B29C64/245 , A61L27/56 , B29C64/00 , B29C35/16 , C12M23/34 , C12M1/12 , C12M25/14 , A61L2400/06 , B29K2105/0002
摘要： A method of producing a cryogel-based multicompartment 3D scaffold is herein disclosed. The method comprises the steps of: a) providing a first frozen polymeric layer on a refrigerated support kept at subzero temperature; b) providing subsequent polymeric layers to obtain a stack of polymeric layers by possibly modulating the subzero temperature of the refrigerated support; c) optionally incubating the final polymeric structure at subzero temperature; and d) placing the produced cryogel at a temperature above 0° C., wherein each subsequent layer i) is deposited on the previous one after freezing of this latter; ii) is deposited on the previous one before the complete polymerization of this latter; and iii) is deposited with a temperature higher than the freezing temperature of the previously deposited layer. Cryogel scaffolds obtained from said method are also disclosed.
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