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公开(公告)号：US10995356B2
公开(公告)日：2021-05-04
申请号：US15569579
申请日：2016-04-26
摘要： The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.
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公开(公告)号：US10414186B2
公开(公告)日：2019-09-17
申请号：US16063472
申请日：2016-12-12
发明人： Eric A. Vandre , Matthew S. Stay , Matthew R. D. Smith , Ann M. Gilman , Shawn C. Dodds , Jon E. Dable , Evan D. Brutinel
摘要： Methods for using a hollow, rotating stencil roll to deposit flowable dry powder particles onto a moving substrate and to attach the particles to the substrate.
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公开(公告)号：US20210114017A1
公开(公告)日：2021-04-22
申请号：US17056884
申请日：2019-05-23
摘要： A microbial detection device and methods of use are provided. The device comprises a water-proof pouch that includes a first wall portion, a second wall portion, and a porous membrane filter disposed in the pouch between the first and second wall portions. The filter membrane divides the pouch into first and second compartments. A dry, cold water-soluble gelling agent is adhered to the pouch in the first compartment and an absorbent pad is disposed in the second compartment. A release liner removably adhered to the first adhesive layer.
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4.发明申请公开(公告)号：US20190001369A1
公开(公告)日：2019-01-03
申请号：US16063460
申请日：2016-12-16
发明人： Evan D. Brutinel , Jon E. Dable , Shawn C. Dodds , Ann M. Gilman , Matthew R.D. Smith , Matthew S. Stay , Eric A. Vandre
摘要： Methods for using a hollow, rotating stencil roll to deposit flowable dry powder particles onto a moving substrate and to adhesively bond the particles to a pressure-sensitive adhesive surface of the substrate.
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公开(公告)号：US20180304576A1
公开(公告)日：2018-10-25
申请号：US15777312
申请日：2016-12-14
发明人： Jonathan E. Janoski , Carla S. Thomas , Anthony F. Schultz , Tien Y.H. Whiting , Kevin M. Lewandowski , Duane D. Fansler , Keith R. Lyon , Arlin L. Weikel , Evan D. Brutinel , Michael W. Kobe , Taylor J. Kobe , Ronald S. Steelman , Eric D. Shockey , John W. Frank
CPC分类号： B32B3/30 , B32B5/022 , B32B5/024 , B32B15/082 , B32B27/065 , B32B27/08 , B32B27/10 , B32B27/12 , B32B27/18 , B32B27/20 , B32B27/26 , B32B27/281 , B32B27/30 , B32B27/304 , B32B27/306 , B32B27/308 , B32B27/32 , B32B27/34 , B32B27/36 , B32B27/365 , B32B27/40 , B32B29/002 , B32B2250/02 , B32B2255/02 , B32B2255/06 , B32B2255/10 , B32B2255/12 , B32B2255/205 , B32B2255/26 , B32B2262/0253 , B32B2262/0261 , B32B2262/04 , B32B2262/062 , B32B2262/101 , B32B2262/105 , B32B2264/102 , B32B2307/31 , B32B2307/4026 , B32B2307/408 , B32B2307/41 , B32B2307/412 , B32B2307/418 , B32B2307/54 , B32B2307/546 , B32B2307/718 , B32B2307/732 , B32B2405/00 , B32B2451/00 , C08J5/08 , C08J5/18 , C08J2323/12 , C08J2333/08 , C08J2429/14 , C08J2433/08 , D06N3/04 , D06N3/042 , D06N2211/28
摘要： A film is described comprising a (meth)acrylic polymer and polyvinyl acetal polymer composition, wherein the film comprises a structured layer.
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公开(公告)号：US20180100131A1
公开(公告)日：2018-04-12
申请号：US15566427
申请日：2016-04-26
CPC分类号： C12M1/045 , C12M1/005 , C12M1/007 , C12M1/14 , C12M23/14 , C12M23/22 , C12M25/02 , C12M25/06 , C12M41/34 , C12M41/36 , C12N1/20 , C12Q1/04
摘要： A device for culturing anaerobic microorganisms is provided. The device comprises a body comprising a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed between the base and the coversheet. The growth compartment has a perimeter and an opening that provides liquid access to the growth compartment. A portion of the perimeter is defined by a waterproof seal. The portion includes >50% of the perimeter. A dry cold water-soluble gelling agent is adhered to the base in the growth compartment. A dry first oxygen-scavenging reagent is disposed in the growth compartment.
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7.发明申请公开(公告)号：US20170355943A1
公开(公告)日：2017-12-14
申请号：US15519944
申请日：2015-11-16
CPC分类号： C12M23/04 , A61L2/081 , A61L2/087 , C12M23/20 , C12M25/06 , C12M41/36 , C12M45/22 , C12Q1/04 , C12Q1/06
摘要： Devices for the propagation or storage of microorganisms are provided including a first layer that has a first portion of a surface of the first layer, to which a first water-swellable gelling agent comprising a first clay is affixed. The devices further include a second layer that is separable from the first layer and has a first portion of a surface of the second layer, to which a second water-swellable gelling agent is affixed. Methods for detecting and enumerating at least one microorganism in a sample are provided. The methods include providing a device, separating the first layer from the second layer, adding an aliquot of a sample containing at least one microorganism onto the first or second water-swellable gelling agent to form an inoculated device, laminating the first layer back to the second layer, and incubating the inoculated device. Kits and methods of making the devices are also provided.
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公开(公告)号：US12077804B2
公开(公告)日：2024-09-03
申请号：US15559349
申请日：2016-07-18
摘要： A microbial detection device and methods of use are provided. The device comprises a water-proof pouch that includes a first wall portion, a second wall portion, and a porous membrane filter disposed in the pouch between the first and second wall portions. The filter membrane divides the pouch into first and second compartments. A dry, cold water-soluble gelling agent is adhered to the pouch in the first compartment and an absorbent pad is disposed in the second compartment. A sealable sample port provides access to deposit a liquid into the first compartment.
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9.发明授权公开(公告)号：US11408023B2
公开(公告)日：2022-08-09
申请号：US16461397
申请日：2017-12-22
发明人： Alexi J. Young , Evan D. Brutinel
摘要： Devices for microbial detection of microorganisms are provided including a body member including a substrate having a first major surface and a second major surface. The device further includes a substantially dry, first microbial growth nutrient composition disposed on a portion of the first major surface, a first adhesive composition adhered to the first microbial growth nutrient composition, and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition. The device also includes a cover sheet attached to the body member, where the cover sheet includes a first major surface facing the body member. Devices including a water-proof pouch are also provided. Methods for detecting and enumerating at least one microorganism in a sample are additionally provided, using the devices.
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公开(公告)号：US20220213421A1
公开(公告)日：2022-07-07
申请号：US17610670
申请日：2020-05-29
摘要： A device for growing microorganisms. The device includes a body member comprising a self-supporting, water-proof substrate having upper and lower surfaces; a hydrophobic spacer element adhered to the upper surface of the substrate forming side walls to retain a predetermined amount of liquid in contact with the substrate, wherein the hydrophobic spacer element has a hole therein; a fluid control film in the hole of the hydrophobic spacer element; a cover sheet having an inner-facing surface and an outer-facing surface, the cover sheet adhered to at least a portion of the body member; and a substantially dry, first microbial growth nutrient composition disposed on a portion of the inner surface of the cover sheet; a first adhesive composition adhered to the first microbial growth nutrient composition; and a cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition.
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