公开(公告)号：US08927263B2

公开(公告)日：2015-01-06

申请号：US13953105

申请日：2013-07-29

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Magdalena Anna Ostrowski ,  Gregory Staples

IPC: C12Q1/34 ,  G01N30/60 ,  G01N30/88

CPC classification number: C12Q1/34 ,  G01N30/6095 ,  G01N2030/8818 ,  G01N2333/924 ,  G01N2400/12 ,  G01N2400/38

Abstract: A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.

Abstract translation: 用于聚糖分析的微流体装置包括去糖基化柱，其包含连接到固体支持物上的糖苷酶; 标记柱，其包含用于与氨基反应的反应性酯，其中所述标记柱位于去糖基化柱的下游; 包括能够分离衍生的聚糖的固定相的分析柱; 以及多个入口/出口端口，其被配置为与开关元件上的通道连接以形成流动路径。

公开(公告)号：US09377468B2

公开(公告)日：2016-06-28

申请号：US14209362

申请日：2014-03-13

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Joel Myerson ,  Craig Daniel Wenger ,  Javier E. Satulovsky

IPC: G01N33/68

CPC classification number: G01N33/6848 ,  G01N2458/15 ,  Y10T436/13 ,  Y10T436/142222 ,  Y10T436/24

Abstract: Compound tags and shifting agents are provided that find use in ion mobility spectrometry (IMS), mass spectrometry (MS), or a combination of IMS and MS, and which can substantially increase separation of multiple components in complex samples and facilitate quantitative and multiplexed analyses. In some cases, the compounds include a linker and a normalizing group, each including a structural unit and separated by a cleaveable group, and a crown ether. Also provided are methods for analyzing peptides in a sample. In some cases, the method includes coupling the compound to peptides which include a terminal guanidinium moiety capable of forming an intra-molecular complex with the crown ether.

Abstract translation: 提供了可用于离子迁移谱（IMS），质谱（MS）或IMS和MS的组合的化合物标签和转移剂，其可以显着增加复杂样品中多个组分的分离并促进定量和多重分析 。 在一些情况下，化合物包括连接体和归一化基团，每个基团包括结构单元并由可裂解基团分离，并且冠醚。 还提供了用于分析样品中的肽的方法。 在一些情况下，该方法包括将化合物与包括能与冠醚形成分子内复合物的末端胍部分的肽偶联。

公开(公告)号：US20150125862A1

公开(公告)日：2015-05-07

申请号：US14590869

申请日：2015-01-06

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Robert A. Ach

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q1/6844 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N33/5308 ,  G01N33/6803 ,  G01N33/6848 ,  Y10T436/143333

Abstract: Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the oligonucleotide to the cross-linked protein to produce a second cross-linked product; d) isolating the second cross-linked product using an affinity tag; and e) analyzing the isolated second cross-linked product. Compounds for performing the method are also provided.

Abstract translation: 本文提供了一种样品分析方法。 在某些实施方案中，所述方法包括：a）使用第一化合物交联细胞的蛋白质以产生包含交联蛋白质和RNA的第一交联产物; b）在第二化合物的寡核苷酸部分与RNA杂交的条件下使第一交联产物和第二化合物接触; c）使第一和第二化合物活化反应，由此共价交联寡核苷酸与交联蛋白质以产生第二交联产物; d）使用亲和标签分离第二交联产物; 和e）分析分离的第二交联产物。 还提供了用于执行该方法的化合物。

公开(公告)号：US20230407379A1

公开(公告)日：2023-12-21

申请号：US18246651

申请日：2021-07-30

Applicant: Agilent Technologies, Inc.

Inventor： Adrienne McCampbell ,  Victor Lim ,  Sean Nasri ,  Brian Phillip Smart

IPC: C12Q1/6841 ,  C12Q1/6806

CPC classification number: C12Q1/6841 ,  C12Q1/6806

Abstract: The present methods and apparatus apply an electric field to a sample in order to prepare it for analysis. In some embodiments, an electric field is applied for drying, staining and coverslipping of tissue sections, providing improved tissue sections for analysis and storage.

公开(公告)号：US20190285522A1

公开(公告)日：2019-09-19

申请号：US16290053

申请日：2019-03-01

Applicant: Agilent Technologies, Inc.

Inventor： Genevieve Van de Bittner ,  Brian Phillip Smart

IPC: G01N1/40 ,  B01D11/04 ,  B01D15/38

Abstract: Methods for removing salts from a metabolite solution. The methods comprise forming an insoluble silver phosphate salt. Further methods include methods for removing hydrolyzed fluorous compounds. These methods comprise extraction with a fluorous solvent in the presence of a protonation reagent and/or chromatography on a fluorous affinity resin. Methods also include separating lysed cell debris and denatured proteins/disrupted enzymes from a metabolite mixture in a container with a filter, where live cells are grown prior to the lysis, either adherent to the filter or in suspension above the filter. The cells are then lysed in the container.

公开(公告)号：US20140273252A1

公开(公告)日：2014-09-18

申请号：US14209362

申请日：2014-03-13

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Joel Myerson ,  Craig Daniel Wenger ,  Javier E. Satulovsky

IPC: G01N33/68

CPC classification number: G01N33/6848 ,  G01N2458/15 ,  Y10T436/13 ,  Y10T436/142222 ,  Y10T436/24

Abstract: Compound tags and shifting agents are provided that find use in ion mobility spectrometry (IMS), mass spectrometry (MS), or a combination of IMS and MS, and which can substantially increase separation of multiple components in complex samples and facilitate quantitative and multiplexed analyses. In some cases, the compounds include a linker and a normalizing group, each including a structural unit and separated by a cleaveable group, and a crown ether. Also provided are methods for analyzing peptides in a sample. In some cases, the method includes coupling the compound to peptides which include a terminal guanidinium moiety capable of forming an intra-molecular complex with the crown ether.

Abstract translation: 提供了可用于离子迁移谱（IMS），质谱（MS）或IMS和MS的组合的化合物标签和转移剂，其可以显着增加复杂样品中多个组分的分离并促进定量和多重分析 。 在一些情况下，化合物包括连接体和归一化基团，每个基团包括结构单元并由可裂解基团分离，并且冠醚。 还提供了用于分析样品中的肽的方法。 在一些情况下，该方法包括将化合物与包括能与冠醚形成分子内复合物的末端胍部分的肽偶联。

公开(公告)号：US09958363B2

公开(公告)日：2018-05-01

申请号：US14724656

申请日：2015-05-28

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Brooks Bond-Watts ,  James Alexander Apffel, Jr.

IPC: G01N1/00 ,  G01N1/34 ,  B01D11/04 ,  G01N1/40

CPC classification number: G01N1/34 ,  B01D11/04 ,  G01N1/405 ,  G01N1/4055 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/24

Abstract: A method for removing an ionic liquid from an aqueous sample is provided. In some embodiments, the method includes: (a) combining an aqueous sample including an ionic liquid with an ion exchanger composition including an ion exchanger counterion to produce a solution including a fluorous salt of the ionic liquid, where at least one of the ionic liquid and the ion exchanger counterion is fluorinated; (b) contacting the solution with a fluorous affinity material, thereby removing fluorous salt from the solution and producing an aqueous eluate; and (c) collecting the aqueous eluate. In certain embodiments, the method further includes: contacting a cell with an ionic liquid composition to lyse the cell and produce an aqueous sample; and contacting the aqueous sample with a reverse phase substrate, thereby adsorbing proteins and/or lipids of the cell on the substrate. Compositions, kits and systems for practicing the subject methods are also provided.

公开(公告)号：US09574236B2

公开(公告)日：2017-02-21

申请号：US14590869

申请日：2015-01-06

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Robert A. Ach

IPC: G01N33/68 ,  C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/6876 ,  C12Q1/6844 ,  C12Q2600/158 ,  C12Q2600/178 ,  G01N33/5308 ,  G01N33/6803 ,  G01N33/6848 ,  Y10T436/143333

Abstract: Provided herein is a method of sample analysis. In certain embodiments, the method comprises: a) cross-linking protein of a cell using a first compound to produce a first cross-linked product comprising cross-linked protein, and RNA; b) contacting the first cross-linked product and a second compound under conditions by which an oligonucleotide portion of the second compound hybridizes to the RNA; c) activating a reaction the first and second compound, thereby covalently crosslinking the oligonucleotide to the cross-linked protein to produce a second cross-linked product; d) isolating the second cross-linked product using an affinity tag; and e) analyzing the isolated second cross-linked product. Compounds for performing the method are also provided.

Abstract translation: 本文提供了一种样品分析方法。 在某些实施方案中，所述方法包括：a）使用第一化合物交联细胞的蛋白质以产生包含交联蛋白质和RNA的第一交联产物; b）在第二化合物的寡核苷酸部分与RNA杂交的条件下使第一交联产物和第二化合物接触; c）使第一和第二化合物活化反应，由此共价交联寡核苷酸与交联蛋白质以产生第二交联产物; d）使用亲和标签分离第二交联产物; 和e）分析分离的第二交联产物。 还提供了用于执行该方法的化合物。

公开(公告)号：US20160258848A1

公开(公告)日：2016-09-08

申请号：US14987256

申请日：2016-01-04

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Kristin Briana Bernick

IPC: G01N1/30 ,  G01N21/78 ,  G01N33/483

Abstract: Provided in this disclosure are methods and compositions that find use in a variety of multiplex cellular/tissue section analyses. In certain aspects, a tissue section (or planar cellular slide) is stained with a combination of “visible” labels and “invisible” labels for specific targets of interest. The visible labels are observed to obtain a result and then, based on the result, one or more of the invisible labels are detected, e.g., using digital microscopy.

Abstract translation: 在本公开中提供了可用于多种细胞/组织切片分析的方法和组合物。 在某些方面，组织部分（或平面细胞载玻片）用特定目的靶标的“可见”标签和“不可见”标签的组合染色。 观察可见标签以获得结果，然后基于结果，检测一个或多个不可见标签，例如使用数字显微镜。

公开(公告)号：US20150369711A1

公开(公告)日：2015-12-24

申请号：US14724656

申请日：2015-05-28

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Brooks Bond-Watts ,  James Alexander Apffel, Jr.

IPC: G01N1/34 ,  B01D11/04

CPC classification number: G01N1/34 ,  B01D11/04 ,  G01N1/405 ,  G01N1/4055 ,  Y10T436/145555 ,  Y10T436/147777 ,  Y10T436/24

Abstract: A method for removing an ionic liquid from an aqueous sample is provided. In some embodiments, the method includes: (a) combining an aqueous sample including an ionic liquid with an ion exchanger composition including an ion exchanger counterion to produce a solution including a fluorous salt of the ionic liquid, where at least one of the ionic liquid and the ion exchanger counterion is fluorinated; (b) contacting the solution with a fluorous affinity material, thereby removing fluorous salt from the solution and producing an aqueous eluate; and (c) collecting the aqueous eluate. In certain embodiments, the method further includes: contacting a cell with an ionic liquid composition to lyse the cell and produce an aqueous sample; and contacting the aqueous sample with a reverse phase substrate, thereby adsorbing proteins and/or lipids of the cell on the substrate. Compositions, kits and systems for practicing the subject methods are also provided.

Abstract translation: 提供了从水样中除去离子液体的方法。 在一些实施方案中，该方法包括：（a）将包含离子液体的含水样品与包含离子交换剂抗衡离子的离子交换剂组合物组合以产生包含离子液体的氟盐的溶液，其中至少一种离子液体 并且离子交换剂抗衡离子被氟化; （b）使溶液与氟亲和材料接触，从而从溶液中除去氟盐并产生水性洗脱液; 和（c）收集水性洗脱液。 在某些实施方案中，所述方法还包括：使细胞与离子液体组合物接触以裂解细胞并产生水样品; 并使水样品与反相底物接触，由此将细胞的蛋白质和/或脂质吸附在基质上。 还提供了用于实践主题方法的组成，成套工具和系统。