公开(公告)号：US20160305919A1

公开(公告)日：2016-10-20

申请号：US15053491

申请日：2016-02-25

Applicant: Agilent Technologies, Inc.

Inventor： Gregory Staples

IPC: G01N30/60 ,  G01N1/40 ,  B01D15/32 ,  C12Q1/34 ,  B01D15/18 ,  B01L3/00 ,  G01N30/88

CPC classification number: G01N30/6095 ,  B01D15/18 ,  B01D15/1871 ,  B01D15/305 ,  B01D15/325 ,  B01L3/502738 ,  B01L3/502753 ,  B01L2200/0631 ,  C12Q1/34 ,  C12Y305/01052 ,  G01N1/405 ,  G01N30/88 ,  G01N2030/8831 ,  G01N2030/8836 ,  G01N2333/98

Abstract: A microfluidic device for glycopeptide analysis includes an enrichment column capable of binding carbohydrates; a trapping column capable of binding peptides, wherein the trapping column is configured to be connected downstream of the enrichment column; a separation column, wherein the separation column is configured to be connected downstream of the trapping column; and a plurality of ports configured to work with a switching device to form a plurality of flow paths, wherein one of the plurality of flow paths allows the trapping column to be in fluid communication with the separation column. A method for glycopeptide analysis using a microfluidic device comprising a trapping column and a separation column, the method includes applying a sample of peptides to the microfluidic device; trapping the peptides on the trapping column; eluting the peptides from the trapping column into the separation column; and separating the peptides on the separation column.

Abstract translation: 用于糖肽分析的微流体装置包括能够结合碳水化合物的富集柱; 能够结合肽的捕集塔，其中捕集塔被配置为连接在浓缩塔的下游; 分离塔，其中所述分离塔被配置为连接在所述捕集塔的下游; 以及多个端口，其被配置为与开关装置一起工作以形成多个流动路径，其中所述多个流动路径中的一个允许所述捕集塔与所述分离塔流体连通。 使用包含捕集柱和分离柱的微流体装置进行糖肽分析的方法，该方法包括将肽样品应用于微流体装置; 将肽捕获在捕集柱上; 将肽从捕集柱洗脱到分离柱中; 并分离分离柱上的肽。

公开(公告)号：US20140038215A1

公开(公告)日：2014-02-06

申请号：US13953105

申请日：2013-07-29

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Magdalena Anna Ostrowski ,  Gregory Staples

IPC: C12Q1/34

CPC classification number: C12Q1/34 ,  G01N30/6095 ,  G01N2030/8818 ,  G01N2333/924 ,  G01N2400/12 ,  G01N2400/38

Abstract: A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.

Abstract translation: 用于聚糖分析的微流体装置包括去糖基化柱，其包含连接到固体支持物上的糖苷酶; 标记柱，其包含用于与氨基反应的反应性酯，其中所述标记柱位于去糖基化柱的下游; 包括能够分离衍生的聚糖的固定相的分析柱; 以及多个入口/出口端口，其被配置为与开关元件上的通道连接以形成流动路径。

公开(公告)号：US08927263B2

公开(公告)日：2015-01-06

申请号：US13953105

申请日：2013-07-29

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Magdalena Anna Ostrowski ,  Gregory Staples

IPC: C12Q1/34 ,  G01N30/60 ,  G01N30/88

CPC classification number: C12Q1/34 ,  G01N30/6095 ,  G01N2030/8818 ,  G01N2333/924 ,  G01N2400/12 ,  G01N2400/38

Abstract: A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.

Abstract translation: 用于聚糖分析的微流体装置包括去糖基化柱，其包含连接到固体支持物上的糖苷酶; 标记柱，其包含用于与氨基反应的反应性酯，其中所述标记柱位于去糖基化柱的下游; 包括能够分离衍生的聚糖的固定相的分析柱; 以及多个入口/出口端口，其被配置为与开关元件上的通道连接以形成流动路径。