公开(公告)号：US10209259B2

公开(公告)日：2019-02-19

申请号：US14664709

申请日：2015-03-20

Applicant: Agilent Technologies, Inc.

Inventor： Magdalena Anna Ostrowski ,  Rudolf Grimm ,  Kevin P. Killeen ,  Karla M. Robotti

IPC: B01L3/00 ,  G01N33/00 ,  G01N33/68 ,  F16K99/00 ,  G01N27/447 ,  G01N30/46 ,  G01N30/60 ,  G01N30/72 ,  B01D15/08 ,  G01N1/40 ,  G01N30/08 ,  H01J49/00

Abstract: Microfluidic devices and methods for analyzing glycan profiles of glycoproteins are provided. Some embodiments of the devices comprise a deglycosylation column for cleaving glycans, an optional cleaning column for removing proteins, a trapping column for enriching glycans, and a separation column for resolving glycans. The devices and methods significantly improve the speed and sensitivity of glycan analysis.

公开(公告)号：US20140038215A1

公开(公告)日：2014-02-06

申请号：US13953105

申请日：2013-07-29

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Magdalena Anna Ostrowski ,  Gregory Staples

IPC: C12Q1/34

CPC classification number: C12Q1/34 ,  G01N30/6095 ,  G01N2030/8818 ,  G01N2333/924 ,  G01N2400/12 ,  G01N2400/38

Abstract: A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.

Abstract translation: 用于聚糖分析的微流体装置包括去糖基化柱，其包含连接到固体支持物上的糖苷酶; 标记柱，其包含用于与氨基反应的反应性酯，其中所述标记柱位于去糖基化柱的下游; 包括能够分离衍生的聚糖的固定相的分析柱; 以及多个入口/出口端口，其被配置为与开关元件上的通道连接以形成流动路径。

公开(公告)号：US20150198611A1

公开(公告)日：2015-07-16

申请号：US14664709

申请日：2015-03-20

Applicant: Agilent Technologies, Inc.

Inventor： Magdalena Anna Ostrowski ,  Rudolf Grimm ,  Kevin P. Killeen ,  Karla M. Robotti

IPC: G01N33/68 ,  G01N27/447 ,  G01N30/72 ,  G01N30/60 ,  G01N30/46 ,  B01D15/08 ,  B01L3/00

CPC classification number: G01N33/6842 ,  B01D15/08 ,  B01L3/502738 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0627 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0644 ,  F16K99/0001 ,  G01N1/405 ,  G01N27/44791 ,  G01N30/465 ,  G01N30/6095 ,  G01N30/7266 ,  G01N2030/085 ,  G01N2400/00 ,  H01J49/00

Abstract: Microfluidic devices and methods for analyzing glycan profiles of glycoproteins are provided. Some embodiments of the devices comprise a deglycosylation column for cleaving glycans, an optional cleaning column for removing proteins, a trapping column for enriching glycans, and a separation column for resolving glycans. The devices and methods significantly improve the speed and sensitivity of glycan analysis.

Abstract translation: 提供了用于分析糖蛋白聚糖谱的微流体装置和方法。 装置的一些实施方案包括用于切割聚糖的去糖基化柱，用于去除蛋白质的可选清洁柱，用于富集聚糖的捕集塔，以及用于分解聚糖的分离柱。 装置和方法显着提高了聚糖分析的速度和灵敏度。

公开(公告)号：US08927263B2

公开(公告)日：2015-01-06

申请号：US13953105

申请日：2013-07-29

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Magdalena Anna Ostrowski ,  Gregory Staples

IPC: C12Q1/34 ,  G01N30/60 ,  G01N30/88

CPC classification number: C12Q1/34 ,  G01N30/6095 ,  G01N2030/8818 ,  G01N2333/924 ,  G01N2400/12 ,  G01N2400/38

Abstract: A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.

Abstract translation: 用于聚糖分析的微流体装置包括去糖基化柱，其包含连接到固体支持物上的糖苷酶; 标记柱，其包含用于与氨基反应的反应性酯，其中所述标记柱位于去糖基化柱的下游; 包括能够分离衍生的聚糖的固定相的分析柱; 以及多个入口/出口端口，其被配置为与开关元件上的通道连接以形成流动路径。