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公开(公告)号：US10012574B2
公开(公告)日：2018-07-03
申请号：US14205100
申请日：2014-03-11
Applicant: Agilent Technologies, Inc.
Inventor： James Alexander Apffel, Jr.
CPC classification number: G01N1/30 , G01N33/5005 , G01N2570/00
Abstract: Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided.
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公开(公告)号：US09958363B2
公开(公告)日：2018-05-01
申请号：US14724656
申请日：2015-05-28
Applicant: Agilent Technologies, Inc.
Inventor： Brian Phillip Smart , Brooks Bond-Watts , James Alexander Apffel, Jr.
CPC classification number: G01N1/34 , B01D11/04 , G01N1/405 , G01N1/4055 , Y10T436/145555 , Y10T436/147777 , Y10T436/24
Abstract: A method for removing an ionic liquid from an aqueous sample is provided. In some embodiments, the method includes: (a) combining an aqueous sample including an ionic liquid with an ion exchanger composition including an ion exchanger counterion to produce a solution including a fluorous salt of the ionic liquid, where at least one of the ionic liquid and the ion exchanger counterion is fluorinated; (b) contacting the solution with a fluorous affinity material, thereby removing fluorous salt from the solution and producing an aqueous eluate; and (c) collecting the aqueous eluate. In certain embodiments, the method further includes: contacting a cell with an ionic liquid composition to lyse the cell and produce an aqueous sample; and contacting the aqueous sample with a reverse phase substrate, thereby adsorbing proteins and/or lipids of the cell on the substrate. Compositions, kits and systems for practicing the subject methods are also provided.
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公开(公告)号：US20150369711A1
公开(公告)日：2015-12-24
申请号：US14724656
申请日：2015-05-28
Applicant: Agilent Technologies, Inc.
Inventor： Brian Phillip Smart , Brooks Bond-Watts , James Alexander Apffel, Jr.
CPC classification number: G01N1/34 , B01D11/04 , G01N1/405 , G01N1/4055 , Y10T436/145555 , Y10T436/147777 , Y10T436/24
Abstract: A method for removing an ionic liquid from an aqueous sample is provided. In some embodiments, the method includes: (a) combining an aqueous sample including an ionic liquid with an ion exchanger composition including an ion exchanger counterion to produce a solution including a fluorous salt of the ionic liquid, where at least one of the ionic liquid and the ion exchanger counterion is fluorinated; (b) contacting the solution with a fluorous affinity material, thereby removing fluorous salt from the solution and producing an aqueous eluate; and (c) collecting the aqueous eluate. In certain embodiments, the method further includes: contacting a cell with an ionic liquid composition to lyse the cell and produce an aqueous sample; and contacting the aqueous sample with a reverse phase substrate, thereby adsorbing proteins and/or lipids of the cell on the substrate. Compositions, kits and systems for practicing the subject methods are also provided.
Abstract translation: 提供了从水样中除去离子液体的方法。 在一些实施方案中，该方法包括：（a）将包含离子液体的含水样品与包含离子交换剂抗衡离子的离子交换剂组合物组合以产生包含离子液体的氟盐的溶液，其中至少一种离子液体 并且离子交换剂抗衡离子被氟化; （b）使溶液与氟亲和材料接触，从而从溶液中除去氟盐并产生水性洗脱液; 和（c）收集水性洗脱液。 在某些实施方案中，所述方法还包括：使细胞与离子液体组合物接触以裂解细胞并产生水样品; 并使水样品与反相底物接触，由此将细胞的蛋白质和/或脂质吸附在基质上。 还提供了用于实践主题方法的组成，成套工具和系统。
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