公开(公告)号：US20140273252A1

公开(公告)日：2014-09-18

申请号：US14209362

申请日：2014-03-13

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Joel Myerson ,  Craig Daniel Wenger ,  Javier E. Satulovsky

IPC: G01N33/68

CPC classification number: G01N33/6848 ,  G01N2458/15 ,  Y10T436/13 ,  Y10T436/142222 ,  Y10T436/24

Abstract: Compound tags and shifting agents are provided that find use in ion mobility spectrometry (IMS), mass spectrometry (MS), or a combination of IMS and MS, and which can substantially increase separation of multiple components in complex samples and facilitate quantitative and multiplexed analyses. In some cases, the compounds include a linker and a normalizing group, each including a structural unit and separated by a cleaveable group, and a crown ether. Also provided are methods for analyzing peptides in a sample. In some cases, the method includes coupling the compound to peptides which include a terminal guanidinium moiety capable of forming an intra-molecular complex with the crown ether.

Abstract translation: 提供了可用于离子迁移谱（IMS），质谱（MS）或IMS和MS的组合的化合物标签和转移剂，其可以显着增加复杂样品中多个组分的分离并促进定量和多重分析 。 在一些情况下，化合物包括连接体和归一化基团，每个基团包括结构单元并由可裂解基团分离，并且冠醚。 还提供了用于分析样品中的肽的方法。 在一些情况下，该方法包括将化合物与包括能与冠醚形成分子内复合物的末端胍部分的肽偶联。

公开(公告)号：US09377468B2

公开(公告)日：2016-06-28

申请号：US14209362

申请日：2014-03-13

Applicant: Agilent Technologies, Inc.

Inventor： Brian Phillip Smart ,  Joel Myerson ,  Craig Daniel Wenger ,  Javier E. Satulovsky

IPC: G01N33/68

CPC classification number: G01N33/6848 ,  G01N2458/15 ,  Y10T436/13 ,  Y10T436/142222 ,  Y10T436/24

Abstract: Compound tags and shifting agents are provided that find use in ion mobility spectrometry (IMS), mass spectrometry (MS), or a combination of IMS and MS, and which can substantially increase separation of multiple components in complex samples and facilitate quantitative and multiplexed analyses. In some cases, the compounds include a linker and a normalizing group, each including a structural unit and separated by a cleaveable group, and a crown ether. Also provided are methods for analyzing peptides in a sample. In some cases, the method includes coupling the compound to peptides which include a terminal guanidinium moiety capable of forming an intra-molecular complex with the crown ether.

Abstract translation: 提供了可用于离子迁移谱（IMS），质谱（MS）或IMS和MS的组合的化合物标签和转移剂，其可以显着增加复杂样品中多个组分的分离并促进定量和多重分析 。 在一些情况下，化合物包括连接体和归一化基团，每个基团包括结构单元并由可裂解基团分离，并且冠醚。 还提供了用于分析样品中的肽的方法。 在一些情况下，该方法包括将化合物与包括能与冠醚形成分子内复合物的末端胍部分的肽偶联。