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公开(公告)号：US08652812B2
公开(公告)日：2014-02-18
申请号：US13681574
申请日：2012-11-20
发明人： Jesse D. Miller
CPC分类号： C12Q1/686 , C12Q1/6818 , C12Q1/6853 , C12Q2563/107 , C12Q2531/113 , C12Q2527/107 , C12Q2525/161
摘要： Methods for amplifying and detecting nucleic acids are described, as well as sets of 5′ labeled oligonucleotides.
摘要翻译： 描述了用于扩增和检测核酸的方法，以及5'标记寡核苷酸的组。
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公开(公告)号：US20170128935A1
公开(公告)日：2017-05-11
申请号：US15309505
申请日：2015-06-08
发明人： Stephen B. Roscoe , Myungchan Kang , Evan D. Brutinel , Jesse D. Miller , Renee K. Cline , Minghua Dai , Hsi-Chou Liu
CPC分类号： B01L3/5029 , B01L3/502 , B01L2200/16 , B01L2300/0825 , B01L2400/0406 , B01L2400/0478 , G01N33/5304
摘要： Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.
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公开(公告)号：US09987633B2
公开(公告)日：2018-06-05
申请号：US15309505
申请日：2015-06-08
发明人： Stephen B. Roscoe , Myungchan Kang , Evan D. Brutinel , Jesse D. Miller , Renee K. Cline , Minghua Dai , Hsi-Chou Liu
CPC分类号： B01L3/5029 , B01L3/502 , B01L2200/16 , B01L2300/0825 , B01L2400/0406 , B01L2400/0478 , G01N33/5304
摘要： Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.
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公开(公告)号：US20140186878A1
公开(公告)日：2014-07-03
申请号：US14008905
申请日：2012-03-05
发明人： Stephen B. Roscoe , Shih-Hung Chou , Stephanie J. Moeller , Jesse D. Miller , Kurt J. Halverson , Jason W. Bjork
IPC分类号： C12Q1/04 , C07H17/075 , C07D409/04 , C07D311/16 , C07D405/04
CPC分类号： C12Q1/04 , C07D311/16 , C07D405/04 , C07D409/04 , C07H17/075 , C09B57/02 , C12Q1/045 , G01N33/525 , G01N33/582
摘要： Compounds are provided that are either fluorogenic or fluorophoric. Compositions and articles that include the compounds are also provided. Additionally, methods of detecting a microorganism using the compounds are provided. The compounds are fluorinated and can be used advantageously under acidic conditions.
摘要翻译： 提供了荧光或荧光的化合物。 还提供了包含化合物的组合物和制品。 另外，提供使用该化合物检测微生物的方法。 化合物是氟化的，并且可以在酸性条件下有利地使用。
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公开(公告)号：US20130171635A1
公开(公告)日：2013-07-04
申请号：US13681574
申请日：2012-11-20
发明人： Jesse D. Miller
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6818 , C12Q1/6853 , C12Q2563/107 , C12Q2531/113 , C12Q2527/107 , C12Q2525/161
摘要： Methods for amplifying and detecting nucleic acids are described, as well as sets of 5′ labeled oligonucleotides.
摘要翻译： 描述了用于扩增和检测核酸的方法，以及5'标记寡核苷酸的组。
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公开(公告)号：US20180243737A1
公开(公告)日：2018-08-30
申请号：US15962391
申请日：2018-04-25
发明人： Stephen B. Roscoe , Myungchan Kang , Evan D. Brutinel , Jesse D. Miller , Renee K. Cline , Minghua Dai , Hsi-Chou Liu
CPC分类号： B01L3/5029 , B01L3/502 , B01L2200/16 , B01L2300/0825 , B01L2400/0406 , B01L2400/0478 , G01N33/5304
摘要： Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.
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公开(公告)号：US09896711B2
公开(公告)日：2018-02-20
申请号：US15226096
申请日：2016-08-02
发明人： Stephen B. Roscoe , Shih-Hung Chou , Stephanie J. Moeller , Jesse D. Miller , Kurt J. Halverson , Jason W. Bjork
IPC分类号： C07D405/04 , C12Q1/04 , C07D311/16 , C07D409/04 , C07H17/075 , G01N33/52 , G01N33/58 , C09B57/02
CPC分类号： C12Q1/04 , C07D311/16 , C07D405/04 , C07D409/04 , C07H17/075 , C09B57/02 , C12Q1/045 , G01N33/525 , G01N33/582
摘要： Compounds are provided that are either fluorogenic or fluorophoric. Compositions and articles that include the compounds are also provided. Additionally, methods of detecting a microorganism using the compounds are provided. The compounds are fluorinated and can be used advantageously under acidic conditions.
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