公开(公告)号：US08540987B2

公开(公告)日：2013-09-24

申请号：US12864018

申请日：2009-01-23

申请人： Masachika Azuma ,  Motohide Takahashi ,  Shunji Kozaki ,  Masafumi Mukamoto ,  Tomoko Kohda ,  Gene Kurosawa ,  Yoshikazu Kurosawa

发明人： Masachika Azuma ,  Motohide Takahashi ,  Shunji Kozaki ,  Masafumi Mukamoto ,  Tomoko Kohda ,  Gene Kurosawa ,  Yoshikazu Kurosawa

IPC分类号： A61K39/395 ,  A61K39/00 ,  C07K16/00 ,  C12P21/08

CPC分类号： C07K16/1282 ,  A61K2039/505 ,  A61K2039/507 ,  C07K2317/21 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92

摘要： Provided herein is a means which is effective for botulism diseases and the prevention of the botulism diseases. Specifically provided is a plurality of human anti-botulinum toxin type-A antibodies having different epitopes from one another. Also specifically provided is a composition for neutralizing botulinum toxin type-A, which comprises a combination of two or more of the antibodies and which has a high neutralizing activity.

摘要翻译： 本文提供了对肉毒中毒疾病和预防肉毒杆菌病有效的手段。 具体提供的是具有彼此具有不同表位的多种人类抗 - 肉毒毒素A型抗体。 还具体提供了用于中和A型肉毒杆菌毒素的组合物，其包含两种或更多种抗体的组合并且具有高中和活性。

公开(公告)号：US20120058124A1

公开(公告)日：2012-03-08

申请号：US13198147

申请日：2011-08-04

申请人： Yoshikazu KUROSAWA ,  Yoshitaka IBA ,  Nobuko OHSHIMA ,  Yoshinobu OKUNO

发明人： Yoshikazu KUROSAWA ,  Yoshitaka IBA ,  Nobuko OHSHIMA ,  Yoshinobu OKUNO

IPC分类号： A61K39/42 ,  C12Q1/70 ,  C12P21/00 ,  C07K16/10 ,  A61P31/16

CPC分类号： C07K16/1018 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/76 ,  C07K2319/00

摘要： Provided is an anti-influenza virus antibody that exhibits neutralizing activity beyond the barrier of the two groups of influenza viruses categorized according to the conservativeness of hemagglutinin amino acids, a method of producing the same, and a test method for determining whether the subject carries the neutralizing antibody.

摘要翻译： 本发明提供了抗血清病毒抗体，其表现出根据血凝素氨基酸的保守性分类的两组流感病毒的屏障之外的中和活性，其生产方法，以及用于确定受试者是否携带 中和抗体。

公开(公告)号：US08101553B1

公开(公告)日：2012-01-24

申请号：US10204773

申请日：2001-02-22

申请人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Yoshitaka Iba ,  Kazuhiko Morino ,  Midori Shinohara ,  Motohide Takahashi ,  Yoshinobu Okuno ,  Kimiyasu Shiraki

发明人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Yoshitaka Iba ,  Kazuhiko Morino ,  Midori Shinohara ,  Motohide Takahashi ,  Yoshinobu Okuno ,  Kimiyasu Shiraki

IPC分类号： C40B50/06

CPC分类号： C12N15/10 ,  C07K16/00 ,  C07K2317/50 ,  C07K2317/56

摘要： An antibody library is prepared by selecting a light chain variable region capable of binding to the variable region of heavy chain to reproduce an active conformation and using the same. Because of being capable of maintaining the diversity of the heavy chain variable region at a high ratio in vitro, the antibody library of the present invention is expected as enabling the acquisition of antibodies with various binding activities.

摘要翻译： 通过选择能够结合重链可变区的轻链可变区以再现活性构象并使用它们来制备抗体文库。 由于能够在体外以高比例保持重链可变区的多样性，本发明的抗体文库预期能够获得具有各种结合活性的抗体。

公开(公告)号：US20050037421A1

公开(公告)日：2005-02-17

申请号：US10489477

申请日：2002-09-13

申请人： Toshio Honda ,  Yasushi Akahori ,  Yoshikazu Kurosawa

发明人： Toshio Honda ,  Yasushi Akahori ,  Yoshikazu Kurosawa

IPC分类号： C07K16/00 ,  C07K16/40 ,  C12Q1/68 ,  C07H21/04 ,  G01N33/53

CPC分类号： C07K16/40 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/22 ,  C07K2317/52 ,  C07K2317/569

摘要： The present invention provides camel antibody libraries that maintain in vivo diversity of camelid antibody variable region genes. The in vivo diversity of antibody variable region genes can be accomplished by, for example, mixing genes derived from a plurality of animals or modifying gene amplification conditions. Conventional methods yield only VHHs with limited repertoire diversity. However, the present invention provides libraries comprising genes encoding functional VHHs with sufficient repertoire size. According to the present invention, libraries that enable to freely obtain VHHs against arbitrary antigens are provided. VHHs have excellent solubility and stability, and show a reactivity that usually cannot be expected from tetrameric IgGs.

摘要翻译： 本发明提供了保持骆驼抗体可变区基因的体内多样性的骆驼抗体文库。 抗体可变区基因的体内多样性可以通过例如混合衍生自多种动物的基因或修饰基因扩增条件来实现。 常规方法仅产生具有有限谱库多样性的VHH。 然而，本发明提供了包含编码具有足够谱库大小的功能性VHH的基因的文库。 根据本发明，提供能够自由获得针对任意抗原的VHH的文库。 VHH具有优异的溶解度和稳定性，并显示通常不能从四聚体IgGs预期的反应性。

公开(公告)号：US08048992B2

公开(公告)日：2011-11-01

申请号：US11885245

申请日：2006-02-22

申请人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsustumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

发明人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsustumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

IPC分类号： C07K16/00

CPC分类号： C07K16/303 ,  A61K2039/505 ,  C07K16/2803 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2319/00 ,  G01N33/57426 ,  G01N33/57438

摘要： It is intended to clarify a molecule which is available as a target in treating or diagnosing cancer and utilize the molecule in the medical field or the research field. By treating IgSF4, which has been identified as a molecule specifically expressed in lung cancer cells, with an antibody, and ADCC activity is exerted. Based on this finding, an anti-IgSF4 antibody is provided as a means efficacious in treating cancer, etc.

摘要翻译： 旨在澄清可用作治疗或诊断癌症并在医学领域或研究领域中利用分子的靶标的分子。 通过用抗体处理被鉴定为特异性表达于肺癌细胞中的分子的IgSF4，并施加ADCC活性。 基于该发现，提供了抗IgSF4抗体作为治疗癌症等的手段。

公开(公告)号：US20090203538A1

公开(公告)日：2009-08-13

申请号：US12318829

申请日：2009-01-09

申请人： Atsushi Sugioka ,  Mototaka Sugiura ,  Yasushi Akahori ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Miwa Morita ,  Gene Kurosawa ,  Mariko Sumitomo ,  Susumu Tsutsumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Noriko Satou ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Suzuki ,  Yoshikazu Kurosawa ,  Miho Tanaka ,  Mamoru Shiraishi

发明人： Atsushi Sugioka ,  Mototaka Sugiura ,  Yasushi Akahori ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Miwa Morita ,  Gene Kurosawa ,  Mariko Sumitomo ,  Susumu Tsutsumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Noriko Satou ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Suzuki ,  Yoshikazu Kurosawa ,  Miho Tanaka ,  Mamoru Shiraishi

IPC分类号： C40B30/04 ,  G01N33/567 ,  G01N33/574 ,  C07K16/00 ,  C40B40/10 ,  C40B60/10

CPC分类号： C07K16/30 ,  C07K16/005 ,  C07K16/28 ,  C07K16/2803 ,  C07K16/2863 ,  C07K16/2896 ,  C07K16/303 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/732 ,  C12N15/1138 ,  C12N2310/14 ,  G01N33/57492 ,  G01N33/6854

摘要： It is intended to provide a method whereby a plural number of antibodies against cell surface antigens are quickly classified and to provide a method whereby antigens of the thus classified antibodies are quickly identified. Further, it is intended to provide a method of promoting the utilization of the useful data obtained by the above methods. Furthermore, it is intended to provide an antibody which is effective in treating or diagnosing cancer. Namely, a method of classifying antibodies which comprises: (1) the step of preparing a plural number of antibodies respectively recognizing cell surface antigens; (2) the step of bringing each of these antibodies into contact with a cell of the same species; (3) the step of analyzing each of the cells having been treated in the step (2) by flow cytometry and thus obtaining data indicating the reactivity of each antibody with its cell surface antigen; and (4) the step of comparing the thus obtained data and classifying the individual antibodies depending on the similarity. A method of identifying antigens which further comprises: (5) the step of selecting one to several antibodies from each antibody group formed in the step (4) and identifying antigens thereof; and (6) on the assumption that antigens of the antibodies belonging to a single antibody group are the same or highly related to one another, making relations between the antigens having been identified in the step (5) and the antibody groups to thereby identify the antigens. An antibody against HER1, an antibody against HER2, an antibody against CD46, an antibody against ITGA3, an antibody against ICAM1, an antibody against ALCAM, an antibody against CD147, an antibody against C1qR, an antibody against CD44, an antibody against CD73, an antibody against EpCAM and an antibody against HGFR, each obtained by using the above methods.

公开(公告)号：US20090053243A1

公开(公告)日：2009-02-26

申请号：US11885245

申请日：2006-02-22

申请人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsustumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

发明人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsustumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

IPC分类号： A61K39/395 ,  C07K16/18 ,  C12N15/11 ,  C12N15/00 ,  A61P31/00 ,  C12N5/06 ,  G01N33/574

CPC分类号： C07K16/303 ,  A61K2039/505 ,  C07K16/2803 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2319/00 ,  G01N33/57426 ,  G01N33/57438

摘要： It is intended to clarify a molecule which is available as a target in treating or diagnosing cancer and utilize the molecule in the medical field or the research field. By treating IgSF4, which has been identified as a molecule specifically expressed in lung cancer cells, with an antibody, and ADCC activity is exerted. Based on this finding, an anti-IgSF4 antibody is provided as a means efficacious in treating cancer, etc.

摘要翻译： 旨在澄清可用作治疗或诊断癌症并在医学领域或研究领域中利用分子的靶标的分子。 通过用抗体处理被鉴定为特异性表达于肺癌细胞中的分子的IgSF4，并施加ADCC活性。 基于该发现，提供了抗IgSF4抗体作为治疗癌症等的手段。

公开(公告)号：US5475100A

公开(公告)日：1995-12-12

申请号：US109106

申请日：1993-08-19

申请人： Kimikazu Hashino ,  Fusao Kimizuka ,  Ikunoshin Kato ,  Yoshikazu Kurosawa ,  Koiti Titani ,  Kiyotoshi Sekiguchi

发明人： Kimikazu Hashino ,  Fusao Kimizuka ,  Ikunoshin Kato ,  Yoshikazu Kurosawa ,  Koiti Titani ,  Kiyotoshi Sekiguchi

IPC分类号： A61K39/395 ,  A61K38/00 ,  A61P37/00 ,  C07K14/00 ,  C07K16/00 ,  C07K16/46 ,  C07K19/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/13 ,  C12P21/02 ,  C12P21/08 ,  C12R1/91 ,  C07H21/00

CPC分类号： C07K16/00 ,  A61K38/00 ,  C07K2317/77

摘要： This invention provides an antibody having antigen binding activity and which has a heavy chain constant region into which has been introduced an RGDS amino acid sequence giving the antibody affinity for cells, including macrophages. The antibody exhibits artificial cell adhesive activity which is the newly expressed activity that results from insertion of the RGDS amino acid sequence. This antibody having artificial cell adhesive activity can accelerate the phagocytosis of macrophages, and can activate other effector cells. Therefore, this antibody contributes to the self-defense mechanism.

摘要翻译： 本发明提供具有抗原结合活性的抗体，其具有重链恒定区，其中已引入RGDS氨基酸序列，其给予对包括巨噬细胞的细胞的抗体亲和力。 抗体表现出人工细胞粘附活性，其是由RGDS氨基酸序列的插入引起的新表达的活性。 具有人造细胞粘附活性的该抗体可以加速巨噬细胞的吞噬作用，并可以激活其他效应细胞。 因此，该抗体有助于自卫机制。

公开(公告)号：US07371849B2

公开(公告)日：2008-05-13

申请号：US10489477

申请日：2002-09-13

申请人： Toshio Honda ,  Yasushi Akahori ,  Yoshikazu Kurosawa

发明人： Toshio Honda ,  Yasushi Akahori ,  Yoshikazu Kurosawa

IPC分类号： C07H21/04 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C07K16/40 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/22 ,  C07K2317/52 ,  C07K2317/569

摘要： The present invention provides camel antibody libraries that maintain in vivo diversity of camelid antibody variable region genes. The in vivo diversity of antibody variable region genes can be accomplished by, for example, mixing genes derived from a plurality of animals or modifying gene amplification conditions. Conventional methods yield only VHHs with limited repertoire diversity. However, the present invention provides libraries comprising genes encoding functional VHHs with sufficient repertoire size. According to the present invention, libraries that enable to freely obtain VHHs against arbitrary antigens are provided. VHHs have excellent solubility and stability, and show a reactivity that usually cannot be expected from tetrameric IgGs.

摘要翻译： 本发明提供了保持骆驼抗体可变区基因的体内多样性的骆驼抗体文库。 抗体可变区基因的体内多样性可以通过例如混合衍生自多种动物的基因或修饰基因扩增条件来实现。 常规方法仅产生具有有限谱库多样性的VHH。 然而，本发明提供了包含编码具有足够谱库大小的功能性VHH的基因的文库。 根据本发明，提供能够自由获得针对任意抗原的VHH的文库。 VHH具有优异的溶解度和稳定性，并显示通常不能从四聚体IgGs预期的反应性。

公开(公告)号：US20120046451A1

公开(公告)日：2012-02-23

申请号：US13238096

申请日：2011-09-21

申请人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsutsumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

发明人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Nobuhiro Takahashi ,  Atsushi Sugioka ,  Nobuhiro Hayashi ,  Akihiko Takasaki ,  Kazuhiro Suzuki ,  Miwa Morita ,  Gene Kurosawa ,  Sari Fujiyama ,  Susumu Tsutsumi ,  Keiko Ogawa ,  Kazuki Matsuda ,  Chiho Muramatsu ,  Yoshitaka Iba ,  Mariko Sumitomo ,  Masachika Azuma ,  Yoshinori Ukai ,  Kazuhiro Morishita

IPC分类号： C07K16/42

CPC分类号： C07K16/303 ,  A61K2039/505 ,  C07K16/2803 ,  C07K2317/52 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622 ,  C07K2317/732 ,  C07K2319/00 ,  G01N33/57426 ,  G01N33/57438

摘要： It is intended to clarify a molecule which is available as a target in treating or diagnosing cancer and utilize the molecule in the medical field or the research field. By treating IgSF4, which has been identified as a molecule specifically expressed in lung cancer cells, with an antibody, and ADCC activity is exerted. Based on this finding, an anti-IgSF4 antibody is provided as a means efficacious in treating cancer, etc.

摘要翻译： 旨在澄清可用作治疗或诊断癌症并在医学领域或研究领域中利用分子的靶标的分子。 通过用抗体处理被鉴定为特异性表达于肺癌细胞中的分子的IgSF4，并施加ADCC活性。 基于该发现，提供了抗IgSF4抗体作为治疗癌症等的手段。