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    Expression vector for animal cells including CSP-B 5′-SAR factor and method for producing recombinant proteins using same
    2.
    发明授权
    Expression vector for animal cells including CSP-B 5′-SAR factor and method for producing recombinant proteins using same 有权
    包括CSP-B 5'-SAR因子在内的动物细胞的表达载体及使用其的重组蛋白质的制备方法

    公开(公告)号：US09228218B2

    公开(公告)日：2016-01-05

    申请号：US14125705

    申请日：2012-05-21

    申请人： Yeo-Wook Koh ,  Sang-Yong Lee ,  Su-Yon Kim ,  Seung-Kee Moon

    发明人： Yeo-Wook Koh ,  Sang-Yong Lee ,  Su-Yon Kim ,  Seung-Kee Moon

    IPC分类号： C12N15/85 ,  C12N9/64 ,  C12P21/00 ,  C12N9/48 ,  C12N9/50 ,  C12P21/04 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C12P21/00 ,  C12N9/6408 ,  C12N15/85 ,  C12N2830/46 ,  C12N2830/85

    摘要： The present invention relates to an expression vector for animal cells, comprising: (a) CSP-B (Cytotoxic Serine Protease-B) 5′-SAR (Scaffold or Matrix Attachment Region); (b) a promoter operable in animal cells; and (c) a polyadenylation sequence, and to a method for producing recombinant proteins using same. The vector of the present invention includes CSP-B 5′-SAR, and thus has the effect of overcoming the inhibition of gene expression according to the position of a foreign gene introduced into an animal cell, and significantly improving the expression rate of a target protein. The vector of the present invention effectively expresses recombinant proteins for drugs or antibodies in animal cells. The vector of the present invention and the method for producing recombinant proteins using same may be very usefully applied to the industrial mass production of drugs.

    摘要翻译： 本发明涉及动物细胞的表达载体，其包含：（a）CSP-B（细胞毒性丝氨酸蛋白酶-B）5'-SAR（支架或基质附着区）; （b）可在动物细胞中操作的启动子; 和（c）聚腺苷酸化序列，以及使用其生产重组蛋白的方法。 本发明的载体包括CSP-B 5'-SAR，因此具有根据引入动物细胞的外源基因的位置克服基因表达的抑制的作用，并显着提高靶的表达率 蛋白。 本发明的载体在动物细胞中有效表达药物或抗体的重组蛋白质。 本发明的载体和使用其的重组蛋白质的制备方法可以非常有效地应用于药物的工业量产。

    EXPRESSION VECTOR FOR ANIMAL CELLS INCLUDING CSP-B 5'-SAR FACTOR AND METHOD FOR PRODUCING RECOMBINANT PROTEINS USING SAME
    3.
    发明申请
    EXPRESSION VECTOR FOR ANIMAL CELLS INCLUDING CSP-B 5'-SAR FACTOR AND METHOD FOR PRODUCING RECOMBINANT PROTEINS USING SAME 有权
    包含CSP-B 5'- SAR因子的动物细胞的表达载体及使用其生产重组蛋白的方法

    公开(公告)号：US20140093914A1

    公开(公告)日：2014-04-03

    申请号：US14125705

    申请日：2012-05-21

    申请人： Yeo-Wook Koh ,  Sang-Yong Lee ,  Su-Yon Kim ,  Seung-kee Moon

    发明人： Yeo-Wook Koh ,  Sang-Yong Lee ,  Su-Yon Kim ,  Seung-kee Moon

    IPC分类号： C12P21/00

    CPC分类号： C12P21/00 ,  C12N9/6408 ,  C12N15/85 ,  C12N2830/46 ,  C12N2830/85

    摘要： The present invention relates to an expression vector for animal cells, comprising: (a) CSP-B (Cytotoxic Serine Protease-B) 5′-SAR (Scaffold or Matrix Attachment Region); (b) a promoter operable in animal cells; and (c) a polyadenylation sequence, and to a method for producing recombinant proteins using same. The vector of the present invention includes CSP-B 5′-SAR, and thus has the effect of overcoming the inhibition of gene expression according to the position of a foreign gene introduced into an animal cell, and significantly improving the expression rate of a target protein. The vector of the present invention effectively expresses recombinant proteins for drugs or antibodies in animal cells. The vector of the present invention and the method for producing recombinant proteins using same may be very usefully applied to the industrial mass production of drugs.

    摘要翻译： 本发明涉及动物细胞的表达载体，其包含：（a）CSP-B（细胞毒性丝氨酸蛋白酶-B）5'-SAR（支架或基质附着区）; （b）可在动物细胞中操作的启动子; 和（c）聚腺苷酸化序列，以及使用其生产重组蛋白的方法。 本发明的载体包括CSP-B 5'-SAR，因此具有根据引入动物细胞的外源基因的位置克服基因表达的抑制的作用，并显着提高靶的表达率 蛋白。 本发明的载体在动物细胞中有效表达药物或抗体的重组蛋白质。 本发明的载体和使用其的重组蛋白质的制备方法可以非常有效地应用于药物的工业量产。

    Streptomyces sp producing tautomycetin and immunosuppressant comprising tautomycetin as active ingredient
    9.
    发明授权
    Streptomyces sp producing tautomycetin and immunosuppressant comprising tautomycetin as active ingredient 失效
    链霉菌sp产生抗菌素和免疫抑制剂，其包含作为活性成分的抗菌素

    公开(公告)号：US06608045B2

    公开(公告)日：2003-08-19

    申请号：US09308482

    申请日：1999-05-17

    申请人： Hyoung Sik Chun ,  Jong Gwan Kim ,  Hung Bae Chang ,  Seung Kee Moon ,  Hyeog Jin Son ,  Chung Il Hong ,  Jung Woo Kim ,  Nam Hyun Lyu

    发明人： Hyoung Sik Chun ,  Jong Gwan Kim ,  Hung Bae Chang ,  Seung Kee Moon ,  Hyeog Jin Son ,  Chung Il Hong ,  Jung Woo Kim ,  Nam Hyun Lyu

    IPC分类号： C12P1702

    CPC分类号： C12R1/465 ,  C12P7/22

    摘要： The present invention relates to Streptomyces sp. producing tautomycetin which possesses an immunosuppressive or antibacterial activities, a process for preparing tautomycetin from the said microorganism, and an immunosuppressant or immunosuppressive pharmaceutical composition comprising tautomycetin as an active ingredient which suppresses interleukin-2 production, CD69 and interleukin-2 receptor(IL-2R) expression on the cell surface, and graft rejection in the organ transplantation. The present inventors isolated a soil microorganism which produces a substance possessing antibiotic and immunosuppressive activities, and identified the said microorganism and substance as a novel Streptomyces sp. and tautomycetin, respectively. Further, the present inventors developed a method of manufacturing the tautomycetin in a large scale from the culture of the said microorganism and discovered that tautomycetin suppresses mouse mixed lymphocyte reaction(MLR) and the expression of IL-2 based on a series of experiments employing cell line transfected with a recombinant gene.

    摘要翻译： 本发明涉及链霉菌属（Streptomyces sp。） 产生具有免疫抑制或抗菌活性的抗菌素，用于从所述微生物制备抗菌素的方法，以及包含抑制白细胞介素2产生的活性成分的免疫抑制剂或免疫抑制剂或免疫抑制药物组合物，CD69和白细胞介素-2受体（IL-2R ）在细胞表面的表达，以及器官移植中的移植排斥反应。 本发明人分离出产生具有抗生素和免疫抑制活性的物质的土壤微生物，并将所述微生物和物质鉴定为新型链霉菌属（Streptomyces sp。）。 和tautomycetin。 此外，本发明人开发了从所述微生物的培养物大规模制造肠胃菌素的方法，并且发现根据使用细胞的一系列实验，互连霉素抑制小鼠混合淋巴细胞反应（MLR）和IL-2的表达 用重组基因转染。