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1.发明授权公开(公告)号：US07751981B2
公开(公告)日：2010-07-06
申请号：US10263901
申请日：2002-10-02
CPC分类号： G06F19/12 , G06F19/18 , Y02A90/26 , Y02E50/343
摘要： The invention provides an in silico model for determining a S. cerevisiae physiological function. The model includes a data structure relating a plurality of S. cerevisiae reactants to a plurality of S. cerevisiae reactions, a constraint set for the plurality of S. cerevisiae reactions, and commands for determining a distribution of flux through the reactions that is predictive of a S. cerevisiae physiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. The invention further provides methods for making an in silico S. cerevisiae model and methods for determining a S. cerevisiae physiological function using a model of the invention.
摘要翻译： 本发明提供了一种用于确定酿酒酵母生理功能的计算机模型。 该模型包括将多个酿酒酵母反应物与多个酿酒酵母反应相关联的数据结构，针对多个酿酒酵母反应的约束集，以及用于确定通过预测的反应的通量分布的命令 酿酒酵母的生理功能。 本发明的模型还可以包括含有表征相关基因或基因的信息的基因数据库。 本发明进一步提供了使用本发明的模型制备计算机酿酒酵母模型和确定酿酒酵母生理功能的方法。
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公开(公告)号：US20090317881A1
公开(公告)日：2009-12-24
申请号：US12374659
申请日：2007-07-19
申请人： Michael Katz , Jochen Förster , Helga David , Hans Peter Schmidt , Malin Sendelius , Sara Peterson Bjørn , Thomas Thomasen Durhuus
发明人： Michael Katz , Jochen Förster , Helga David , Hans Peter Schmidt , Malin Sendelius , Sara Peterson Bjørn , Thomas Thomasen Durhuus
CPC分类号： C12P7/22 , C12N9/1029 , C12N9/88 , C12N9/93
摘要： A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.
摘要翻译： 具有产生肉桂酰辅酶A的功能性代谢途径并通过二苯乙烯合酶的作用从其生产pinosylvin的遗传工程微生物被用于pinosylvin生产。 所说的肉桂酸可以由L-苯丙氨酸氨裂解酶（PAL）形成，L-苯丙氨酸氨裂解酶（PAL）是一种接受苯丙氨酸作为底物并由其生产肉桂酸，优选使得如果PAL也接受酪氨酸作为底物并形成香豆酸 所述PAL的比例Km（苯丙氨酸）/ Km（酪氨酸）小于1:1，如果所述微生物产生肉桂酸-4-羟化酶（C4H），则比例Kcat（PAL）/ Kcat（C4H ）至少为2：1。
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公开(公告)号：US09040269B2
公开(公告)日：2015-05-26
申请号：US12081571
申请日：2008-04-17
IPC分类号： C12P7/22 , C12N1/00 , C07H21/04 , C12P7/42 , C12P23/00 , C12N9/10 , C12N9/02 , C12N9/88 , C12N9/00 , C12N15/52
CPC分类号： C12P7/22 , A23C3/08 , A61K31/05 , C12N9/0042 , C12N9/0073 , C12N9/1029 , C12N9/1037 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/42 , C12P23/00 , C12Y106/02004 , C12Y114/13011 , C12Y203/01074 , C12Y403/01005 , C12Y602/01012 , C12Y604/01002 , Y02A50/473 , Y02P20/52
摘要： A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.
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公开(公告)号：US09822374B2
公开(公告)日：2017-11-21
申请号：US13700011
申请日：2011-05-24
IPC分类号： C12N15/81 , C12N1/18 , C07K14/37 , C07K14/39 , C12N9/10 , C12N9/90 , C12P7/22 , A23K10/18 , C07K14/395 , C12N9/00
CPC分类号： C12N15/81 , A23K10/18 , C07K14/37 , C07K14/395 , C12N1/18 , C12N9/1029 , C12N9/93 , C12P7/22
摘要： A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.
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公开(公告)号：US20100280803A1
公开(公告)日：2010-11-04
申请号：US12769555
申请日：2010-04-28
CPC分类号： G06F19/12 , G06F19/18 , Y02A90/26 , Y02E50/343
摘要： The invention provides an in silico model for determining a S. cerevisiae physiological function. The model includes a data structure relating a plurality of S. cerevisiae reactants to a plurality of S. cerevisiae reactions, a constraint set for the plurality of S. cerevisiae reactions, and commands for determining a distribution of flux through the reactions that is predictive of a S. cerevisiae physiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. The invention further provides methods for making an in silico S. cerevisiae model and methods for determining a S. cerevisiae physiological function using a model of the invention.
摘要翻译： 本发明提供了一种用于确定酿酒酵母生理功能的计算机模型。 该模型包括将多个酿酒酵母反应物与多个酿酒酵母反应相关联的数据结构，针对多个酿酒酵母反应的约束集，以及用于确定通过预测的反应的通量分布的命令 酿酒酵母的生理功能。 本发明的模型还可以包括含有表征相关基因或基因的信息的基因数据库。 本发明进一步提供了使用本发明的模型制备计算机酿酒酵母模型和确定酿酒酵母生理功能的方法。
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公开(公告)号：US08518677B2
公开(公告)日：2013-08-27
申请号：US12670709
申请日：2008-07-25
申请人： Hans Peter Schmidt , Michael Katz , Bo Stenhuus , Jochen Förster
发明人： Hans Peter Schmidt , Michael Katz , Bo Stenhuus , Jochen Förster
摘要： A cis- or trans-stilbenoid of the general formula (1): in which each of R1, R2, R3, R4 and R5 is hydrogen or hydroxy, or a glycosylated or oligomeric form thereof, is produced by cultivating a micro-organism producing said stilbenoid, in a multi-phase system comprising at least an aqueous first phase containing said micro-organism and a second phase immiscible with said aqueous phase in which (e.g. as which) said stilbenoid accumulates. The second phase may be said stilbenoid or a free or encapsulated solvent compatible with the growth of the micro-organism, for instance an ester.
摘要翻译： 通式（1）的顺式或反式芪类化合物：其中R 1，R 2，R 3，R 4和R 5各自为氢或羟基，或其糖基化或寡聚形式，是通过培养产生微生物 所述螺旋体在多相系统中包含至少含有所述微生物的含水第一相和与所述水相不混溶的第二相，其中（例如，其中）所述茋被积聚。 第二相可以是所述萜类化合物或与微生物例如酯的生长相容的游离或包囊的溶剂。
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公开(公告)号：US08343739B2
公开(公告)日：2013-01-01
申请号：US12374659
申请日：2007-07-19
申请人： Michael Katz , Jochen Förster , Helga David , Hans Peter Schmidt , Malin Sendelius , Sara Peterson Bjørn , Thomas Thomasen Durhuus
发明人： Michael Katz , Jochen Förster , Helga David , Hans Peter Schmidt , Malin Sendelius , Sara Peterson Bjørn , Thomas Thomasen Durhuus
IPC分类号： C12P7/22
CPC分类号： C12P7/22 , C12N9/1029 , C12N9/88 , C12N9/93
摘要： A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.
摘要翻译： 具有产生肉桂酰辅酶A的功能性代谢途径并通过二苯乙烯合酶的作用从其生产pinosylvin的遗传工程微生物被用于pinosylvin生产。 所说的肉桂酸可以由L-苯丙氨酸氨裂解酶（PAL）形成，L-苯丙氨酸氨裂解酶（PAL）是一种接受苯丙氨酸作为底物并由其生产肉桂酸，优选使得如果PAL也接受酪氨酸作为底物并形成香豆酸 所述PAL的比例Km（苯丙氨酸）/ Km（酪氨酸）小于1:1，如果所述微生物产生肉桂酸-4-羟化酶（C4H），则比例Kcat（PAL）/ Kcat（C4H ）至少为2：1。
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8.发明授权公开(公告)号：US08170852B2
公开(公告)日：2012-05-01
申请号：US12769555
申请日：2010-04-28
CPC分类号： G06F19/12 , G06F19/18 , Y02A90/26 , Y02E50/343
摘要： The invention provides an in silico model for determining a S. cerevisiae physiological function. The model includes a data structure relating a plurality of S. cerevisiae reactants to a plurality of S. cerevisiae reactions, a constraint set for the plurality of S. cerevisiae reactions, and commands for determining a distribution of flux through the reactions that is predictive of a S. cerevisiae physiological function. A model of the invention can further include a gene database containing information characterizing the associated gene or genes. The invention further provides methods for making an in silico S. cerevisiae model and methods for determining a S. cerevisiae physiological function using a model of the invention.
摘要翻译： 本发明提供了一种用于确定酿酒酵母生理功能的计算机模型。 该模型包括将多个酿酒酵母反应物与多个酿酒酵母反应相关联的数据结构，针对多个酿酒酵母反应的约束集，以及用于确定通过预测的反应的通量分布的命令 酿酒酵母的生理功能。 本发明的模型还可以包括含有表征相关基因或基因的信息的基因数据库。 本发明进一步提供了使用本发明的模型制备计算机酿酒酵母模型和确定酿酒酵母生理功能的方法。
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公开(公告)号：US20100203603A1
公开(公告)日：2010-08-12
申请号：US12670709
申请日：2008-07-25
申请人： Hans Peter Schmidt , Michael Katz , Bo Stenhuus , Jochen Förster
发明人： Hans Peter Schmidt , Michael Katz , Bo Stenhuus , Jochen Förster
摘要： A cis- or trans-stilbenoid of the general formula (1): in which each of R1, R2, R3, R4 and R5 is hydrogen or hydroxy, or a glycosylated or oligomeric form thereof, is produced by cultivating a micro-organism producing said stilbenoid, in a multi-phase system comprising at least an aqueous first phase containing said micro-organism and a second phase immiscible with said aqueous phase in which (e.g. as which) said stilbenoid accumulates. The second phase may be said stilbenoid or a free or encapsulated solvent compatible with the growth of the micro-organism, for instance an ester.
摘要翻译： 通式（1）的顺式或反式芪类化合物：其中R 1，R 2，R 3，R 4和R 5各自为氢或羟基，或其糖基化或寡聚形式，是通过培养产生微生物 所述螺旋体在多相系统中包含至少含有所述微生物的含水第一相和与所述水相不混溶的第二相，其中（例如，所述）所述茋被积聚。 第二相可以是所述萜类化合物或与微生物例如酯的生长相容的游离或包囊的溶剂。
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10.发明授权Metabolically engineered cells for the production of resveratrol or an oligomeric or glycosidically-bound derivative thereof 有权用于生产白藜芦醇或其寡聚或糖苷结合衍生物的代谢工程细胞公开(公告)号：US08895287B2
公开(公告)日：2014-11-25
申请号：US11816847
申请日：2006-02-21
IPC分类号： C12N1/20 , C12N1/00 , C12P7/22 , C07H21/04 , C12P7/42 , C12N9/02 , C12N9/10 , C12N9/88 , C12N9/00 , C12N15/52
CPC分类号： C12P7/22 , A23C3/08 , A61K31/05 , C12N9/0042 , C12N9/0073 , C12N9/1029 , C12N9/1037 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/42 , C12P23/00 , C12Y106/02004 , C12Y114/13011 , C12Y203/01074 , C12Y403/01005 , C12Y602/01012 , C12Y604/01002 , Y02A50/473 , Y02P20/52
摘要： A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.
摘要翻译： 通过苯丙氨酸氨裂解酶（PAL）从苯丙氨酸产生反式肉桂酸，肉桂酸4-羟化酶（C4H）的途径产生白藜芦醇的重组微生物从所述反式肉桂酸，4-香豆酸 - 辅酶A产生4-香豆酸 连接酶（4CL）从所述4-香豆酸生成4-香豆酰CoA，白藜芦醇合酶（VST）从所述4-香豆酰CoA或其中L-苯丙氨酸或酪氨酸 - 氨裂解酶（PAL / TAL）产生白藜芦醇 4-香豆酸，4-香豆酸-CaA连接酶（4CL）从所述4-香豆酸生成4-香豆酰CoA，白藜芦醇合酶（VST）从所述4-香豆酰CoA产生所述白藜芦醇。 微生物可以是酵母，真菌或细菌，包括酿酒酵母，大肠杆菌，乳酸乳球菌，黑曲霉或米曲霉。
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