公开(公告)号：US20140024862A1

公开(公告)日：2014-01-23

申请号：US13732887

申请日：2013-01-02

申请人： Michael Katz ,  Jochen Forster ,  Helga David ,  Hans Peter Schmidt ,  Malin Sendelius ,  Sara Peterson Bjorn ,  Thomas Thomasen Durhuus

发明人： Michael Katz ,  Jochen Forster ,  Helga David ,  Hans Peter Schmidt ,  Malin Sendelius ,  Sara Peterson Bjorn ,  Thomas Thomasen Durhuus

IPC分类号： C12P7/22

CPC分类号： C12P7/22 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/93

摘要： A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.

摘要翻译： 具有产生肉桂酰辅酶A的功能性代谢途径并通过二苯乙烯合酶的作用从其生产pinosylvin的遗传工程微生物被用于pinosylvin生产。 所说的肉桂酸可以由L-苯丙氨酸氨裂解酶（PAL）形成，L-苯丙氨酸氨裂解酶（PAL）是一种接受苯丙氨酸作为底物并由其生产肉桂酸，优选使得如果PAL也接受酪氨酸作为底物并形成香豆酸 所述PAL的比例Km（苯丙氨酸）/ Km（酪氨酸）小于1:1，如果所述微生物产生肉桂酸-4-羟化酶（C4H），则比例Kcat（PAL）/ Kcat（C4H ）至少为2：1。

公开(公告)号：US20090317881A1

公开(公告)日：2009-12-24

申请号：US12374659

申请日：2007-07-19

申请人： Michael Katz ,  Jochen Förster ,  Helga David ,  Hans Peter Schmidt ,  Malin Sendelius ,  Sara Peterson Bjørn ,  Thomas Thomasen Durhuus

发明人： Michael Katz ,  Jochen Förster ,  Helga David ,  Hans Peter Schmidt ,  Malin Sendelius ,  Sara Peterson Bjørn ,  Thomas Thomasen Durhuus

IPC分类号： C12P7/22 ,  C12N1/19

CPC分类号： C12P7/22 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/93

摘要： A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.

摘要翻译： 具有产生肉桂酰辅酶A的功能性代谢途径并通过二苯乙烯合酶的作用从其生产pinosylvin的遗传工程微生物被用于pinosylvin生产。 所说的肉桂酸可以由L-苯丙氨酸氨裂解酶（PAL）形成，L-苯丙氨酸氨裂解酶（PAL）是一种接受苯丙氨酸作为底物并由其生产肉桂酸，优选使得如果PAL也接受酪氨酸作为底物并形成香豆酸 所述PAL的比例Km（苯丙氨酸）/ Km（酪氨酸）小于1:1，如果所述微生物产生肉桂酸-4-羟化酶（C4H），则比例Kcat（PAL）/ Kcat（C4H ）至少为2：1。

公开(公告)号：US08343739B2

公开(公告)日：2013-01-01

申请号：US12374659

申请日：2007-07-19

申请人： Michael Katz ,  Jochen Förster ,  Helga David ,  Hans Peter Schmidt ,  Malin Sendelius ,  Sara Peterson Bjørn ,  Thomas Thomasen Durhuus

发明人： Michael Katz ,  Jochen Förster ,  Helga David ,  Hans Peter Schmidt ,  Malin Sendelius ,  Sara Peterson Bjørn ,  Thomas Thomasen Durhuus

IPC分类号： C12P7/22

CPC分类号： C12P7/22 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/93

摘要： A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.

摘要翻译： 具有产生肉桂酰辅酶A的功能性代谢途径并通过二苯乙烯合酶的作用从其生产pinosylvin的遗传工程微生物被用于pinosylvin生产。 所说的肉桂酸可以由L-苯丙氨酸氨裂解酶（PAL）形成，L-苯丙氨酸氨裂解酶（PAL）是一种接受苯丙氨酸作为底物并由其生产肉桂酸，优选使得如果PAL也接受酪氨酸作为底物并形成香豆酸 所述PAL的比例Km（苯丙氨酸）/ Km（酪氨酸）小于1:1，如果所述微生物产生肉桂酸-4-羟化酶（C4H），则比例Kcat（PAL）/ Kcat（C4H ）至少为2：1。

公开(公告)号：US20110086399A1

公开(公告)日：2011-04-14

申请号：US12937508

申请日：2009-04-08

申请人： Hans Peter Smits ,  Helga David ,  Jochen Forster ,  Bo Stenhuus

发明人： Hans Peter Smits ,  Helga David ,  Jochen Forster ,  Bo Stenhuus

IPC分类号： C12P7/22

CPC分类号： C12P7/22

摘要： A cis- or trans-stilbenoid of the general formula (1) in which each of R1, R2, R3, R4 and R5 is hydrogen or hydroxy, or a glycosylated or oligomeric form thereof, such as resveratrol or pinosylvin is produced by cultivating a microorganism such as a genetically engineered yeast to produce said stilbenoid in a culture medium in solid form, and is separated by filtration or settling.

摘要翻译： 其中R1，R2，R3，R4和R5各自为氢或羟基的通式（1）的顺式或反式芪类化合物，或其糖基化或寡聚体形式，如白藜芦醇或豆蔻糖醇，是通过培养 微生物如遗传工程酵母，以固体形式在培养基中产生所述茋，并通过过滤或沉降分离。