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    Metabolically Engineered Cells For The Production of Pinosylvin
    1.
    发明申请
    Metabolically Engineered Cells For The Production of Pinosylvin 审中-公开
    用于生产Pinosylvin的代谢工程细胞

    公开(公告)号：US20140024862A1

    公开(公告)日：2014-01-23

    申请号：US13732887

    申请日：2013-01-02

    申请人： Michael Katz ,  Jochen Forster ,  Helga David ,  Hans Peter Schmidt ,  Malin Sendelius ,  Sara Peterson Bjorn ,  Thomas Thomasen Durhuus

    发明人： Michael Katz ,  Jochen Forster ,  Helga David ,  Hans Peter Schmidt ,  Malin Sendelius ,  Sara Peterson Bjorn ,  Thomas Thomasen Durhuus

    IPC分类号： C12P7/22

    CPC分类号： C12P7/22 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/93

    摘要： A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.

    摘要翻译： 具有产生肉桂酰辅酶A的功能性代谢途径并通过二苯乙烯合酶的作用从其生产pinosylvin的遗传工程微生物被用于pinosylvin生产。 所说的肉桂酸可以由L-苯丙氨酸氨裂解酶（PAL）形成，L-苯丙氨酸氨裂解酶（PAL）是一种接受苯丙氨酸作为底物并由其生产肉桂酸，优选使得如果PAL也接受酪氨酸作为底物并形成香豆酸 所述PAL的比例Km（苯丙氨酸）/ Km（酪氨酸）小于1:1，如果所述微生物产生肉桂酸-4-羟化酶（C4H），则比例Kcat（PAL）/ Kcat（C4H ）至少为2：1。

    RESISTIVE PRESSURE MEASURING CELL HAVING DIAGNOSTIC CAPABILITIES
    4.
    发明申请
    RESISTIVE PRESSURE MEASURING CELL HAVING DIAGNOSTIC CAPABILITIES 审中-公开
    具有诊断能力的电阻式压力测量单元

    公开(公告)号：US20130118264A1

    公开(公告)日：2013-05-16

    申请号：US13812157

    申请日：2011-08-30

    申请人： Heinz Walter ,  Oliver Blankenhorn ,  Jochen Forster

    发明人： Heinz Walter ,  Oliver Blankenhorn ,  Jochen Forster

    IPC分类号： G01L9/00

    CPC分类号： G01L9/00 ,  G01L9/0055 ,  G01L27/007

    摘要： A pressure measuring cell for detecting the pressure prevailing in an adjoining medium, comprising an elastic membrane on which a first electromechanical transducer is arranged, which supplies a first pressure-dependent output signal is provided. According to the invention, a second electromechanical transducer, which supplies a second pressure-dependent output signal, is arranged on the membrane wherein the two transducers are arranged such that with an elastically reversible deformation of the membrane the output signals have a first pressure characteristic, and after an irreversible deformation of the membrane due to an increased pressure load same have a significantly different second pressure characteristic.

    摘要翻译： 一种压力测量单元，用于检测相邻介质中的压力，该压力测量单元包括一个弹性膜，其上布置有第一机电换能器，其提供第一压力相关的输出信号。 根据本发明，提供第二压力相关输出信号的第二机电换能器布置在膜上，其中两个换能器被布置成使得随着膜的弹性可逆变形，输出信号具有第一压力特性， 并且在由于增加的压力负载引起的膜的不可逆变形之后，具有显着不同的第二压力特性。

    Production of Metabolites
    5.
    发明申请
    Production of Metabolites 有权
    生产代谢物

    公开(公告)号：US20130209613A1

    公开(公告)日：2013-08-15

    申请号：US13700011

    申请日：2011-05-24

    申请人： Michael Patrik Katz ,  Thomas Durhuus ,  Hans Peter Smits ,  Jochen Forster

    发明人： Michael Patrik Katz ,  Thomas Durhuus ,  Hans Peter Smits ,  Jochen Forster

    IPC分类号： C12N15/81 ,  A23K1/00

    CPC分类号： C12N15/81 ,  A23K10/18 ,  C07K14/37 ,  C07K14/395 ,  C12N1/18 ,  C12N9/1029 ,  C12N9/93 ,  C12P7/22

    摘要： A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.

    摘要翻译： 一种重组微生物，例如酿酒酵母，其在生长在萜类生产条件下生长并分泌培养基的芪类代谢产物，其在上述天然水平中表达将所述唾液酸从所述微生物细胞转运至培养物的ABC转运蛋白 中。 酿酒酵母的基因组产生营养缺陷型表型，其由表达构成所述代谢途径的一种或多种所述酶的质粒补偿，产生所述茋，质粒的表达产物经遗传修饰以包括泛素标记序列。 与其天然表达水平相比，任选地通过Ehrlich途径表达参与苯丙氨酸分解代谢的酶。

    RECOVERY OF STILBENOIDS
    6.
    发明申请
    RECOVERY OF STILBENOIDS 审中-公开
    恢复STILBENOIDS

    公开(公告)号：US20110086399A1

    公开(公告)日：2011-04-14

    申请号：US12937508

    申请日：2009-04-08

    申请人： Hans Peter Smits ,  Helga David ,  Jochen Forster ,  Bo Stenhuus

    发明人： Hans Peter Smits ,  Helga David ,  Jochen Forster ,  Bo Stenhuus

    IPC分类号： C12P7/22

    CPC分类号： C12P7/22

    摘要： A cis- or trans-stilbenoid of the general formula (1) in which each of R1, R2, R3, R4 and R5 is hydrogen or hydroxy, or a glycosylated or oligomeric form thereof, such as resveratrol or pinosylvin is produced by cultivating a microorganism such as a genetically engineered yeast to produce said stilbenoid in a culture medium in solid form, and is separated by filtration or settling.

    摘要翻译： 其中R1，R2，R3，R4和R5各自为氢或羟基的通式（1）的顺式或反式芪类化合物，或其糖基化或寡聚体形式，如白藜芦醇或豆蔻糖醇，是通过培养 微生物如遗传工程酵母，以固体形式在培养基中产生所述茋，并通过过滤或沉降分离。

    Metabolically engineered cells for the production of polyunsaturated fatty acids
    8.
    发明申请
    Metabolically engineered cells for the production of polyunsaturated fatty acids 失效
    用于生产多不饱和脂肪酸的代谢工程细胞

    公开(公告)号：US20060051847A1

    公开(公告)日：2006-03-09

    申请号：US11146428

    申请日：2005-06-06

    申请人： Nina Gunnarsson ,  Jochen Forster ,  Jens Nielsen

    发明人： Nina Gunnarsson ,  Jochen Forster ,  Jens Nielsen

    IPC分类号： C12P7/64 ,  C12N1/18 ,  C12N15/74

    CPC分类号： C12N15/52 ,  A23L33/12 ,  A61K8/361 ,  A61K8/67 ,  A61Q19/00 ,  C11B1/00 ,  C12N9/0083 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6472 ,  C12P7/6481 ,  Y02P20/582

    摘要： The present invention relates to the construction and engineering of cells, more particularly microorganisms for producing PUFAs with four or more double bonds from non-fatty acid substrates through heterologous expression of an oxygen requiring pathway. The invention especially involves improvement of the PUFA content in the host organism through fermentation optimization, e.g. decreasing the temperature and/or designing an optimal medium, or through improving the flux towards fatty acids by metabolic engineering, e.g. through over-expression of fatty acid synthases, over-expression of other enzymes involved in biosynthesis of the precursors for PUFAs, or codon optimization of the heterologous genes, or expression of heterologous enzymes involved in the biosynthesis of the precursor for PUFAs.

    摘要翻译： 本发明涉及细胞的构建和工程，更特别地涉及通过异氧表达需氧途径从非脂肪酸底物生产具有四个或更多个双键的PUFA的细胞的构建和工程。 本发明特别涉及通过发酵优化来改善宿主生物体中的PUFA含量，例如， 降低温度和/或设计最佳培养基，或通过代谢工程改善通向脂肪酸的通量。 通过脂肪酸合成酶的过表达，参与PUFA前体生物合成的其他酶的过度表达，或异源基因的密码子优化，或参与PUFA前体生物合成的异源酶的表达。