发明申请
- 专利标题: Production of Metabolites
- 专利标题(中): 生产代谢物
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申请号: US13700011申请日: 2011-05-24
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公开(公告)号: US20130209613A1公开(公告)日: 2013-08-15
- 发明人: Michael Patrik Katz , Thomas Durhuus , Hans Peter Smits , Jochen Forster
- 申请人: Michael Patrik Katz , Thomas Durhuus , Hans Peter Smits , Jochen Forster
- 申请人地址: DK Stenlose
- 专利权人: FLUXOME SCIENCES A/S
- 当前专利权人: FLUXOME SCIENCES A/S
- 当前专利权人地址: DK Stenlose
- 优先权: GB1008826.8 20100526
- 国际申请: PCT/EP2011/058447 WO 20110524
- 主分类号: C12N15/81
- IPC分类号: C12N15/81 ; A23K1/00
摘要:
A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.
公开/授权文献
- US09822374B2 Production of metabolites 公开/授权日:2017-11-21
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