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    Type II restriction endonuclease SexAI
    3.
    发明授权
    Type II restriction endonuclease SexAI 失效
    II型限制性内切酶SexAI

    公开(公告)号：US5354669A

    公开(公告)日：1994-10-11

    申请号：US94374

    申请日：1993-07-19

    申请人： Klaus Kaluza ,  Johannes Auer ,  Bruno Frey

    发明人： Klaus Kaluza ,  Johannes Auer ,  Bruno Frey

    IPC分类号： C12N9/22 ,  C12P19/34

    CPC分类号： C12N9/22

    摘要： The new type II restriction endonuclease SexAI has the following recognition sequence and preferably cleaves at the cleavage site defined by the mark: ##STR1## It is preferably obtainable from microorganisms of the genus Streptomyces.

    摘要翻译： 新型II型限制性内切核酸酶SexAI具有以下识别序列，优选在由标记：链霉菌属的无机物定义的切割位点切割。

    Method for the recombinant expression of an N-terminal fragment of hepatocyte growth factor
    6.
    发明授权
    Method for the recombinant expression of an N-terminal fragment of hepatocyte growth factor 失效
    重组表达肝细胞生长因子N-末端片段的方法

    公开(公告)号：US07754448B2

    公开(公告)日：2010-07-13

    申请号：US10591045

    申请日：2005-03-02

    申请人： Johannes Auer ,  Apollon Papadimitriou ,  Christian Schantz ,  Stefan Seeber

    发明人： Johannes Auer ,  Apollon Papadimitriou ,  Christian Schantz ,  Stefan Seeber

    IPC分类号： C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C07K14/4753 ,  C12N15/70

    摘要： A method for the production of the α-chain of hepatocyte growth factor or an N-terminal fragment thereof (NK polypeptide) by expression of a nucleic acid encoding said NK polypeptide in a microbial host cell, isolating inclusion bodies containing said NK polypeptide in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK polypeptide, which is characterized in that in said nucleic acid at least one of the codons of amino acids selected from the group consisting of codons at positions 33, 35 and 36 is CGT, results in an improved expression yield.

    摘要翻译： 通过在微生物宿主细胞中表达编码所述NK多肽的核酸，通过将包含所述NK多肽的包含体分离成变性来生产肝细胞生长因子或其N-末端片段（NK多肽）的α-链的方法 形式，包涵体的溶解和变性NK多肽的生成，其特征在于在所述核酸中，选自33,35和36位的密码子中的至少一个氨基酸的密码子是CGT， 导致表达产率提高。