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    Reagentless and reusable biosensors with tunable differential binding affinities and methods of making
    1.
    发明授权
    Reagentless and reusable biosensors with tunable differential binding affinities and methods of making 有权
    具有可调节差异结合亲和力和制备方法的无菌和可重复使用的生物传感器

    公开(公告)号：US07927547B2

    公开(公告)日：2011-04-19

    申请号：US12103778

    申请日：2008-04-16

    申请人： Igor L Medintz ,  J Matthew Mauro ,  Ellen R Goldman ,  George P Anderson

    发明人： Igor L Medintz ,  J Matthew Mauro ,  Ellen R Goldman ,  George P Anderson

    IPC分类号： G01N21/00 ,  G01N21/01 ,  G01N21/64 ,  G01N33/53 ,  G01N33/553

    CPC分类号： C12Q1/6818 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N2021/6441 ,  C12Q2565/607 ,  C12Q2565/101

    摘要： The biosensor comprises a modular biorecognition element and a modular flexible arm element. The biorecognition element and the flexible arm element are each labeled with a signaling element. The flexible arm contains an analog of an analyte of interest that binds with the biorecognition element, bringing the two signaling elements in close proximity, which establishes a baseline fluorescence resonance energy transfer (FRET). When an analyte of interest is provided to the biosensor, the analyte will displace the analyte analog, and with it, the signaling module of the modular flexible arm, causing a measurable change in the FRET signal in a analyte concentration dependent manner. The modularity of different portions of the biosensor allows functional flexibility. The biosensor operates without additional development reagents, requiring only the presence of analyte or target for function.

    摘要翻译： 生物传感器包括模块化生物识别元件和模块化柔性臂元件。 生物识别元件和柔性臂元件各自用信号元件标记。 柔性臂包含与生物识别元件结合的感兴趣分析物的类似物，使得两个信号元件紧密相邻，这建立了基线荧光共振能量转移（FRET）。 当感兴趣的分析物被提供给生物传感器时，分析物将置换分析物类似物，并且借助于该模块化柔性臂的信号模块，以分析​​物浓度依赖的方式引起FRET信号的可测量的变化。 生物传感器的不同部分的模块化允许功能灵活性。 生物传感器无需额外的开发试剂即可运行，仅需要分析物或靶标的存在才能发挥作用。

    Reagentless and Reusable Biosensors with Tunable Differential Binding Affinities and Methods of Making
    2.
    发明申请
    Reagentless and Reusable Biosensors with Tunable Differential Binding Affinities and Methods of Making 有权
    具有可调节差异结合亲和力的制剂和可重复使用的生物传感器和制备方法

    公开(公告)号：US20090048123A1

    公开(公告)日：2009-02-19

    申请号：US12103778

    申请日：2008-04-16

    申请人： Igor L. Medintz ,  Ellen R. Goldman ,  George P. Anderson ,  J. Matthew Mauro

    发明人： Igor L. Medintz ,  Ellen R. Goldman ,  George P. Anderson ,  J. Matthew Mauro

    IPC分类号： C40B40/04 ,  G01N21/64 ,  C12M1/00 ,  C40B50/00

    CPC分类号： C12Q1/6818 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N2021/6441 ,  C12Q2565/607 ,  C12Q2565/101

    摘要： The biosensor comprises a modular biorecognition element and a modular flexible arm element. The biorecognition element and the flexible arm element are each labeled with a signaling element. The flexible arm contains an analog of an analyte of interest that binds with the biorecognition element, bringing the two signaling elements in close proximity, which establishes a baseline fluorescence resonance energy transfer (FRET). When an analyte of interest is provided to the biosensor, the analyte will displace the analyte analog, and with it, the signaling module of the modular flexible arm, causing a measurable change in the FRET signal in a analyte concentration dependent manner. The modularity of different portions of the biosensor allows functional flexibility. The biosensor operates without additional development reagents, requiring only the presence of analyte or target for function.

    摘要翻译： 生物传感器包括模块化生物识别元件和模块化柔性臂元件。 生物识别元件和柔性臂元件各自用信号元件标记。 柔性臂包含与生物识别元件结合的感兴趣分析物的类似物，使得两个信号元件紧密相邻，这建立了基线荧光共振能量转移（FRET）。 当感兴趣的分析物被提供给生物传感器时，分析物将置换分析物类似物，并且借助于该模块化柔性臂的信号模块，以分析​​物浓度依赖的方式引起FRET信号的可测量的变化。 生物传感器的不同部分的模块化允许功能灵活性。 生物传感器无需额外的开发试剂即可运行，仅需要分析物或靶标的存在才能发挥作用。

    Use of Modular Nucleic Acid Scaffolds to Create Nanoscale Energy Harvesting and Focusing Arrays
    7.
    发明申请
    Use of Modular Nucleic Acid Scaffolds to Create Nanoscale Energy Harvesting and Focusing Arrays 有权
    使用模块化核酸支架创建纳米级能量收集和聚焦数组

    公开(公告)号：US20150293022A1

    公开(公告)日：2015-10-15

    申请号：US14685836

    申请日：2015-04-14

    申请人： Susan Buckhout-White ,  Mario Ancona ,  Ellen R. Goldman ,  Igor L. Medintz ,  Joseph S. Melinger

    发明人： Susan Buckhout-White ,  Mario Ancona ,  Ellen R. Goldman ,  Igor L. Medintz ,  Joseph S. Melinger

    IPC分类号： G01N21/64 ,  C12Q1/68

    CPC分类号： C12Q1/6818 ,  G01N21/6428 ,  C12Q2563/155

    摘要： The invention relates to a nanoscale antenna including a nucleic acid scaffold having a structure selected from the group consisting of a Holliday junction, a star, and a dendrimer; and a plurality of fluorophores attached to the scaffold and configured as a FRET cascade comprising at least three different types of fluorophores, arranged with (a) a plurality of initial donor fluorophores fixed in exterior positions on the structure, (b) a terminal acceptor fluorophore fixed in a central position on the structure, and (c) a plurality of intermediate fluorophores fixed in positions on the scaffold between the initial acceptor fluorophores and the terminal acceptor fluorophores.

    摘要翻译： 本发明涉及纳米尺度天线，其包括具有选自Holliday结，星形和树枝状大分子的结构的核酸支架; 和多个荧光团，其连接到支架并被配置为包含至少三种不同类型的荧光团的FRET级联，其布置为（a）固定在结构上的外部位置的多个初始供体荧光团，（b）末端受体荧光团 固定在结构上的中心位置，和（c）固定在起始受体荧光团和末端受体荧光团之间的支架上的位置上的多个中间荧光团。

    Reagentless and reusable biosensors with tunable differential binding affinities and methods of making
    9.
    发明授权
    Reagentless and reusable biosensors with tunable differential binding affinities and methods of making 有权
    具有可调节差异结合亲和力和制备方法的无菌和可重复使用的生物传感器

    公开(公告)号：US07435386B2

    公开(公告)日：2008-10-14

    申请号：US11094540

    申请日：2005-03-25

    申请人： Igor L. Medintz ,  Ellen R. Goldman ,  George P. Anderson ,  J. Matthew Mauro

    发明人： Igor L. Medintz ,  Ellen R. Goldman ,  George P. Anderson ,  J. Matthew Mauro

    IPC分类号： G01N21/00 ,  G01N21/01 ,  G01N21/64 ,  G01N33/53 ,  G01N33/553

    CPC分类号： C12Q1/6818 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N2021/6441 ,  C12Q2565/607 ,  C12Q2565/101

    摘要： The biosensor comprises a modular biorecognition element and a modular flexible arm element. The biorecognition element and the flexible arm element are each labeled with a signaling element. The flexible arm contains an analog of an analyte of interest that binds with the biorecognition element, bringing the two signaling elements in close proximity, which establishes a baseline fluorescence resonance energy transfer (FRET). When an analyte of interest is provided to the biosensor, the analyte will displace the analyte analog, and with it, the signaling module of the modular flexible arm, causing a measurable change in the FRET signal in a analyte concentration dependent manner. The modularity of different portions of the biosensor allows functional flexibility. The biosensor-operates without additional development reagents, requiring only the presence of analyte or target for function.

    摘要翻译： 生物传感器包括模块化生物识别元件和模块化柔性臂元件。 生物识别元件和柔性臂元件各自用信号元件标记。 柔性臂包含与生物识别元件结合的感兴趣分析物的类似物，使得两个信号元件紧密相邻，这建立了基线荧光共振能量转移（FRET）。 当感兴趣的分析物被提供给生物传感器时，分析物将置换分析物类似物，并且借助于该模块化柔性臂的信号模块，以分析​​物浓度依赖的方式引起FRET信号的可测量的变化。 生物传感器的不同部分的模块化允许功能灵活性。 生物传感器无需额外的开发试剂即可进行操作，仅需要分析物或靶标的功能。