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1.发明申请NANOSPLASMONIC IMAGING TECHNIQUE FOR THE SPATIO-TEMPORAL MAPPING OF SINGLE CELL SECRETIONS IN REAL TIME 有权用于实时单细胞分析的空间时间映射的纳米平面成像技术公开(公告)号：US20140273002A1
公开(公告)日：2014-09-18
申请号：US14207927
申请日：2014-03-13
IPC分类号： G01N33/569
CPC分类号： G01N21/554 , G01N21/274 , G01N21/59 , G01N21/6458 , G01N21/648 , G01N33/54373 , G01N2021/6491
摘要： A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.
摘要翻译： 通过使用用于局部表面等离子体共振（LSPR）成像的芯片实时地对来自各个细胞的蛋白质分泌物进行时空映射的无标记方法。 该芯片是一种玻璃盖玻片，适用于标准显微镜，具有至少一个图案化的功能化等离子体纳米结构阵列。 将细胞置于芯片上后，使用LSPR成像在空间和时间上映射来自细胞的分泌。 透射光成像和/或荧光成像可以与LSPR成像同时进行。
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公开(公告)号：US09791368B2
公开(公告)日：2017-10-17
申请号：US14207927
申请日：2014-03-13
IPC分类号： G01N33/551 , G01N21/552 , G01N33/543 , G01N21/64 , G01N21/59 , G01N21/27
CPC分类号： G01N21/554 , G01N21/274 , G01N21/59 , G01N21/6458 , G01N21/648 , G01N33/54373 , G01N2021/6491
摘要： A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.
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3.发明申请公开(公告)号：US20140095100A1
公开(公告)日：2014-04-03
申请号：US14039326
申请日：2013-09-27
IPC分类号： G01N33/483
CPC分类号： G01N33/483 , B82Y15/00 , G01N21/276 , G01N21/554
摘要： A method for calibrating multiple nanostructures in parallel for quantitative biosensing using a chip for localized surface plasmon resonance (LSPR) biosensing and imaging. The chip is a glass coverslip compatible for use in a standard microscope with at least one array of functionalized plasmonic nanostructures patterned onto it using electron beam nanolithography. The chip is used to collect CCD-based LSPR imagery data of each individual nanostructure and LSPR spectral data of the array. The spectral data is used to determine the fractional occupancy of the array. The imagery data is modeled as a function of fractional occupancy to determine the fractional occupancy of each individual nanostructure.
摘要翻译： 用于使用用于局部表面等离子体共振（LSPR）生物传感和成像的芯片来并行校准多个纳米结构以进行定量生物传感的方法。 该芯片是玻璃盖玻片，可用于标准显微镜，其中至少一个功能化的等离子体激元纳米结构阵列使用电子束纳米光刻法图案化。 该芯片用于收集阵列中各个纳米结构和LSPR光谱数据的基于CCD的LSPR图像数据。 光谱数据用于确定阵列的分数占用。 图像数据被建模为分数占用的函数，以确定每个单个纳米结构的分数占有率。
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公开(公告)号：US10345287B2
公开(公告)日：2019-07-09
申请号：US14039326
申请日：2013-09-27
IPC分类号： G01N21/27 , G01N21/552 , G01N33/483 , B82Y15/00
摘要： A method for calibrating multiple nanostructures in parallel for quantitative biosensing using a chip for localized surface plasmon resonance (LSPR) biosensing and imaging. The chip is a glass coverslip compatible for use in a standard microscope with at least one array of functionalized plasmonic nanostructures patterned onto it using electron beam nanolithography. The chip is used to collect CCD-based LSPR imagery data of each individual nanostructure and LSPR spectral data of the array. The spectral data is used to determine the fractional occupancy of the array. The imagery data is modeled as a function of fractional occupancy to determine the fractional occupancy of each individual nanostructure.
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公开(公告)号：US09915654B2
公开(公告)日：2018-03-13
申请号：US14039288
申请日：2013-09-27
IPC分类号： G01N33/551 , G01N33/557 , G01N33/543 , G01N21/552
CPC分类号： G01N33/557 , G01N21/554 , G01N33/54346 , G01N33/54373 , G01N33/54386
摘要： A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.
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公开(公告)号：US09778265B2
公开(公告)日：2017-10-03
申请号：US14688010
申请日：2015-04-16
IPC分类号： G01N33/68 , C07K14/00 , C12N15/62 , G01N33/531 , G01N33/543 , C07K19/00 , C07K16/16
CPC分类号： G01N33/68 , C07K14/00 , C07K16/16 , C07K19/00 , C07K2317/569 , C07K2319/73 , C12N15/62 , G01N33/531 , G01N33/54393 , G01N2333/42
摘要： Genetic fusions of proteins, for example single-domain antibodies (sdAbs), with a positively-charged domain enhanced immobilization of active protein in a desired orientation.
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7.发明申请Charged Peptide Appendage to Facilitate Oriented Protein Covalent Immobilization 有权带电肽附件促进定向蛋白质共价固定公开(公告)号：US20160305948A1
公开(公告)日：2016-10-20
申请号：US14688010
申请日：2015-04-16
IPC分类号： G01N33/68
CPC分类号： G01N33/68 , C07K14/00 , C07K16/16 , C07K19/00 , C07K2317/569 , C07K2319/73 , C12N15/62 , G01N33/531 , G01N33/54393 , G01N2333/42
摘要： Genetic fusions of proteins, for example single-domain antibodies (sdAbs), with a positively-charged domain enhanced immobilization of active protein in a desired orientation.
摘要翻译： 具有带正电荷结构域的蛋白质的遗传融合，例如单结构域抗体（sdAb）增强了活性蛋白在期望取向上的固定化。
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8.发明申请LIGHT MICROSCOPY CHIPS AND DATA ANALYSIS METHODOLOGY FOR QUANTITATIVE LOCALZIED SURFACE PLASMON RESONANCE (LSPR) BIOSENSING AND IMAGING 有权用于定量局部表面等离子体共振（LSPR）生物传感和成像的光学显微镜和数据分析方法公开(公告)号：US20140093977A1
公开(公告)日：2014-04-03
申请号：US14039288
申请日：2013-09-27
IPC分类号： G01N33/557
CPC分类号： G01N33/557 , G01N21/554 , G01N33/54346 , G01N33/54373 , G01N33/54386
摘要： A chip for localized surface plasmon resonance (LSPR) biosensing and imaging having a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip with electron beam nanolithography. The nanostructures can be regenerated allowing the chip to be used multiple times. Also disclosed is a method for determining the fractional occupancy values for surface-bound receptors as a function of time for LSPR biosensing from the spectroscopic response of the array and modeling the photon count in each spectrometer channel, allowing for a functional relationship to be determined between the acquired spectrum and the fractional occupancy of binding sites on the array. Additionally disclosed is a method for the spatiotemporal mapping of receptor-ligand binding kinetics in LSPR imaging using the chip and projecting a magnified image of the array to a CCD camera and monitoring the binding kinetics of the array.
摘要翻译： 用于局部表面等离子体共振（LSPR）生物传感和成像的芯片具有兼容用于标准显微镜的玻璃盖玻片和用电子束纳米光刻图案化到玻璃盖玻片上的至少一个功能化等离子体激元纳米结构阵列。 纳米结构可以再生，允许芯片多次使用。 还公开了一种用于根据阵列的光谱响应确定表面结合受体的分数占有率作为LSPR生物传感时间的函数的方法，并且对每个光谱仪通道中的光子计数进行建模，从而允许在每个光谱仪通道之间确定功能关系 获得的光谱和阵列上结合位点的分数占有率。 另外公开了使用该芯片的LSPR成像中的受体 - 配体结合动力学的时空映射的方法，并将该阵列的放大图像投影到CCD照相机并监测阵列的结合动力学。
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