公开(公告)号：US20080166809A1

公开(公告)日：2008-07-10

申请号：US11791126

申请日：2005-11-22

申请人： Kunihiro Ohta ,  Hidetaka Seo ,  Kouji Hirota ,  Takehiko Shibata

发明人： Kunihiro Ohta ,  Hidetaka Seo ,  Kouji Hirota ,  Takehiko Shibata

IPC分类号： C12N15/01 ,  C12N1/00

CPC分类号： C12N15/905 ,  C12N9/16 ,  C12N15/81

摘要： The present invention relates to a method for increasing genetic recombination frequency in a genomic DNA and a method for inducing genome rearrangement. Specifically, according to the present invention there are provided: the method for increasing genetic recombination frequency in a cell in which genetic recombination takes place at any sites in the genome, comprising causing a restriction enzyme to be expressed in the cell, inducing transient activation of the restriction enzyme, and then introducing 2 or more double strand cleavages into any genomic DNA of the cell, so as to increase the genetic recombination frequency; the method for inducing genome rearrangement through the use of the above method; and cells each prepared through the use of the above 2 methods.

摘要翻译： 本发明涉及提高基因组DNA中基因重组频率的方法和诱导基因组重排的方法。 具体地说，根据本发明，提供了在基因组中的任何位置发生遗传重组的细胞中增加遗传重组频率的方法，包括使限制性内切酶在细胞中表达，引起瞬时激活 限制酶，然后将2个以上的双链裂解引入细胞的任何基因组DNA，以增加遗传重组频率; 通过使用上述方法诱导基因组重排的方法; 每个细胞通过使用上述2种方法制备。

公开(公告)号：US20080032404A1

公开(公告)日：2008-02-07

申请号：US11780132

申请日：2007-07-19

申请人： Frederick Roth ,  Oliver King ,  Lan Zhang

发明人： Frederick Roth ,  Oliver King ,  Lan Zhang

IPC分类号： C12N15/87

CPC分类号： C12N15/905 ,  C12N15/102 ,  C12N15/65 ,  C12N2510/00

摘要： The present invention provides methods for engineering multiple genomic deletions and/or insertions in eukaryotic cells.

摘要翻译： 本发明提供了在真核细胞中设计多个基因组缺失和/或插入的方法。

公开(公告)号：US20050136447A1

公开(公告)日：2005-06-23

申请号：US10954721

申请日：2004-09-30

申请人： Stephen Elledge ,  Qinghua Liu

发明人： Stephen Elledge ,  Qinghua Liu

IPC分类号： C12N15/10 ,  C12N15/66 ,  C12N15/74 ,  C12N15/90 ,  C12Q1/68 ,  C12N15/85 ,  C12P21/04

CPC分类号： C12N15/905 ,  C12N15/10 ,  C12N15/66 ,  C12N15/74

摘要： The present invention provides compositions, including vectors, and methods for the rapid subcloning of nucleic acid sequences in vivo and in vitro. In particular, the invention provides vectors used to contain a gene of interest that comprise a sequence-specific recombinase target site. These vectors are used to rapidly transfer the gene or genes of interest into any vector that contains a sequence-specific recombinase target site located downstream of a regulatory element so that the gene of interest may be regulated.

摘要翻译： 本发明提供了在体内和体外快速亚克隆核酸序列的组合物，包括载体和方法。 特别地，本发明提供了用于含有包含序列特异性重组酶靶位点的目的基因的载体。 这些载体用于将感兴趣的基因或基因快速转移到任何载体中，所述载体含有位于调节元件下游的序列特异性重组酶靶位点，以使感兴趣的基因可以调节。

公开(公告)号：US06582944B1

公开(公告)日：2003-06-24

申请号：US09184965

申请日：1999-01-08

申请人： Johan Hallborn ,  Merja Penttilä ,  Heikki Ojamo ,  Mats Walfridsson ,  Ulla Airaksinen ,  Sirkka Keränen ,  Bärbel Hahn-Hägerdal

发明人： Johan Hallborn ,  Merja Penttilä ,  Heikki Ojamo ,  Mats Walfridsson ,  Ulla Airaksinen ,  Sirkka Keränen ,  Bärbel Hahn-Hägerdal

IPC分类号： C12P706

CPC分类号： C12P19/02 ,  C12N9/0004 ,  C12N9/0006 ,  C12N15/52 ,  C12N15/81 ,  C12N15/905 ,  C12P7/06 ,  C12P7/18 ,  C12P7/40 ,  Y02E50/17 ,  Y02P20/582

摘要： This invention relates to recombinant-DNA-technology. Specifically this invention relates to new recombinant yeast strains transformed with xylose reductase and/or xylitol dehydrogenase enzyme genes. A yeast strain transformed with the xylose reductase gene is capable of reducing xylose to xylitol and consequently of producing xylitol in vivo. If both of these genes are transformed into a yeast strain, the resultant strain is capable of producing ethanol on xylose containing medium during fermentation. Further, the said new yeast strains are capable of expressing the said two enzymes. Xylose reductase produced by these strains can be used in an enzymatic process for the production of xylitol in vitro.

摘要翻译： 本发明涉及重组DNA技术。 具体地说，本发明涉及用木糖还原酶和/或木糖醇脱氢酶基因转化的新的重组酵母菌株。 用木糖还原酶基因转化的酵母菌株能够将木糖还原成木糖醇，从而在体内产生木糖醇。 如果将这两个基因转化成酵母菌株，则所得菌株能够在发酵期间在含有木糖的培养基上产生乙醇。 此外，所述新酵母菌株能够表达所述两种酶。 由这些菌株产生的木糖还原酶可用于体外生产木糖醇的酶法。

公开(公告)号：US20020146793A1

公开(公告)日：2002-10-10

申请号：US10107384

申请日：2002-03-26

申请人： CHIRON S.p.A. , a Corporation

发明人： Cesira Galeotti

IPC分类号： C12N009/02 ,  C12N009/90 ,  A01K067/00 ,  C12P021/02 ,  C12N001/21 ,  C12N001/18 ,  C12N015/74

CPC分类号： C07K14/005 ,  C07K14/52 ,  C12N9/0036 ,  C12N9/90 ,  C12N15/905 ,  C12N2770/36122 ,  C12P21/02 ,  C12Y108/01008 ,  C12Y503/04001

摘要： An expression system which provides heterologous proteins expressed by a non-native host organism but which have native-protein-like biological activity and/or structure. Disclosed are vectors, expression hosts and methods for expressing the heterologous proteins. The expression system involves co-expression of protein factor(s) which is/are capable of catalyzing disulphide bond formation and desired heterologous protein(s). The expression system is presented using yeast cells as the preferred host, protein disulphide isomerase (PDI) and thioredoxin (TRX) as the preferred examples of the protein factors and HCV-E2715 envelope glycoprotein and human FIGF as the preferred examples of the heterologous proteins.

公开(公告)号：US20020115220A1

公开(公告)日：2002-08-22

申请号：US09908855

申请日：2001-07-20

发明人： Keiji Kondo ,  Yutaka Miura

IPC分类号： C12N015/74 ,  C12N001/18

CPC分类号： C12N9/2411 ,  C07K14/43 ,  C12N9/2408 ,  C12N15/815 ,  C12N15/905 ,  C12Y302/01001

摘要： An object of the present invention is to provide a vector which can be integrated into a yeast chromosome in a high number of copies. Another object of the present invention is to provide a modified vector which can be integrated into the yeast chromosome in a high number of copies and of which expression units stably maintain on the chromosome. The vector according to the present invention comprises a marker gene for selecting transformants, a shortened promoter sequence which is operably linked to the marker gene and a sequence homologous to the chromosomal DNA of Candida utilis, and optionally a heterologous gene or a gene derived from C. utilis, wherein the vector is linearized by cleaving within said homologous DNA sequence or at both ends of the homologous DNA sequence with restriction enzymes, and wherein the heterologous gene or the gene derived from C. utilis can be integrated into the chromosomal DNA of C. utilis by homologous recombination.

摘要翻译： 本发明的目的是提供一种能够以大量拷贝结合到酵母染色体中的载体。 本发明的另一个目的是提供一种修饰的载体，其能够以大量的拷贝整合到酵母染色体中，并且其表达单位稳定地保持在染色体上。 根据本发明的载体包含用于选择转化体的标记基因，可操作地连接到标记基因的缩短的启动子序列和与产朊假丝酵母的染色体DNA同源的序列，以及任选的异源基因或衍生自C的基因 其中载体通过在所述同源DNA序列内切割或用限制性内切酶在同源DNA序列的两端进行线性化，其中异源基因或源自产朊酵母的基因可以整合入C的染色体DNA中 通过同源重组产生。

公开(公告)号：US06284534B1

公开(公告)日：2001-09-04

申请号：US09242690

申请日：1999-02-23

申请人： Keiji Kondo ,  Yutaka Miura

发明人： Keiji Kondo ,  Yutaka Miura

IPC分类号： C12N1581

CPC分类号： C12N9/2411 ,  C07K14/43 ,  C12N9/2408 ,  C12N15/815 ,  C12N15/905 ,  C12Y302/01001

摘要： An object of the present invention is to provide a vector which can be integrated into a yeast chromosome in a high number of copies. Another object of the present invention is to provide a modified vector which can be integrated into the yeast chromosome in a high number of copies and of which expression units stably maintain on the chromosome. The vector according to the present invention comprises a marker gene for selecting transformants, a shortened promoter sequence which is operably linked to the marker gene and a sequence homologous to the chromosomal DNA of Candida utilis, and optionally a heterologous gene or a gene derived from C. utilis, wherein the vector is linearized by cleaving within said homologous DNA sequence or at both ends of the homologous DNA sequence with restriction enzymes, and wherein the heterologous gene or the gene derived from C. utilis can be integrated into the chromosomal DNA of C. utilis by homologous recombination.

摘要翻译： 本发明的目的是提供一种能够以大量拷贝结合到酵母染色体中的载体。 本发明的另一个目的是提供一种修饰的载体，其能够以大量的拷贝整合到酵母染色体中，并且其表达单位稳定地保持在染色体上。 根据本发明的载体包含用于选择转化体的标记基因，可操作地连接到标记基因的缩短的启动子序列和与产朊假丝酵母的染色体DNA同源的序列，以及任选的异源基因或衍生自C的基因 其中载体通过在所述同源DNA序列内切割或用限制性内切酶在同源DNA序列的两端进行线性化，其中异源基因或源自产朊酵母的基因可以整合入C的染色体DNA中 通过同源重组产生。

公开(公告)号：US06235499B1

公开(公告)日：2001-05-22

申请号：US09515326

申请日：2000-02-29

申请人： Hideki Tohda ,  Yuko Hama

发明人： Hideki Tohda ,  Yuko Hama

IPC分类号： C12P2106

CPC分类号： C12N15/905 ,  C12N15/815

摘要： A method for transforming Schizosaccharomyces pombe which comprises integrating a vector into a chromosome of Schizosaccharomyces pombe through homologous recombination, wherein the vector has an expression cassette containing a heterologous protein structural gene and a promoter and a gene segment which induces homologous recombination of the chromosome and has lost a replication origin which functions in cells of an organism other than Schizosaccharomyces pombe required for construction of the vector.

摘要翻译： 一种用于转化粟酒裂殖酵母的方法，其包括通过同源重组将载体整合到粟酒裂殖酵母的染色体中，其中所述载体具有含有异源蛋白质结构基因的表达盒，以及诱导染色体同源重组并具有 失去了在构建载体所需的除了粟酒裂殖酵母之外的生物体的细胞中起作用的复制起点。

公开(公告)号：US5976795A

公开(公告)日：1999-11-02

申请号：US771602

申请日：1996-12-20

申请人： Daniel F. Voytas ,  Sige Zou

发明人： Daniel F. Voytas ,  Sige Zou

IPC分类号： A61K48/00 ,  C12N15/81 ,  C12N15/90 ,  C12Q1/68 ,  C07H21/04 ,  C12N1/16 ,  C12N15/87

CPC分类号： C12N15/905 ,  C12N15/81 ,  A61K48/00

摘要： The present disclosure provides retrotransposons and retrotransposon derivatives and methods for their uses. Specifically, the present invention provides Ty5-6p and derivatives. Ty5-6p and its derivatives integrate preferentially in the genome of eukaryotes in silent chromatin and in regions like silent chromatin. Ty5-6p insertions can be used to regulate the life span of cells, to genetically mark cells, to deliver gene therapy and to identify genes involved in development and in senescence.

摘要翻译： 本公开提供了其用途的反转录转座子和反转录转座子衍生物和方法。 具体地，本发明提供Ty5-6p及其衍生物。 Ty5-6p及其衍生物优先整合到沉默染色质中的真核生物基因组中，在沉默染色质区域。 Ty5-6p插入可用于调节细胞的寿命，遗传标记细胞，提供基因治疗和鉴定参与发育和衰老的基因。

公开(公告)号：US20240110208A1

公开(公告)日：2024-04-04

申请号：US18189272

申请日：2023-03-24

申请人： NATIONAL TSING HUA UNIVERSITY ,  Chang Chun Plastics Co., Ltd. ,  Chang Chun Petrochemical Co., LTD. ,  DAIREN CHEMICAL CORP.

发明人： Yu-Chen HU ,  Nam Ngoc PHAM ,  June-Yen CHOU ,  Hsing-Yun WANG ,  Vincent Jianan LIU

IPC分类号： C12P7/6409 ,  C12N15/52 ,  C12N15/90

CPC分类号： C12P7/6409 ,  C12N15/52 ,  C12N15/905 ,  C12Y101/01 ,  C12Y101/0302 ,  C12Y114/14 ,  C12Y207/01086

摘要： A gene editing system of Candida viswanathii includes a Candida viswanathii, a first gene editing fragment and a second gene editing fragment. The first gene editing fragment successively includes a first homology arm and a screening gene. The second gene editing fragment is connected to a C-terminus of the first gene editing fragment and includes a second homology arm, a Cas9 expression cassette and a sgRNA cassette. The Cas9 expression cassette successively includes a Cas9 promoter, a Cas9 gene and three nuclear localization sequences. The sgRNA cassette successively includes a sgRNA promoter, a first ribozyme, a targeting sequence, a scaffold and a second ribozyme. The first gene editing fragment and the second gene editing fragment are constructed as a linear fragment for gene editing of a chromosome of the Candida viswanathii.