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    Engineering fungi for the utilisation of L-arabinose
    6.
    发明授权
    Engineering fungi for the utilisation of L-arabinose 失效
    工程真菌利用L-阿拉伯糖

    公开(公告)号：US07527951B2

    公开(公告)日：2009-05-05

    申请号：US10257821

    申请日：2002-02-15

    申请人： John Londesborough ,  Merja Penttilä ,  Peter Richard

    发明人： John Londesborough ,  Merja Penttilä ,  Peter Richard

    IPC分类号： C12P7/06 ,  C12N1/18 ,  C12N1/16 ,  C12N15/74

    CPC分类号： C12N9/0006 ,  C12Y101/0101 ,  C12Y101/01012 ,  Y02E50/17

    摘要： A fungal microorganism can be engineered by means of genetic engineering to utilise L-arabinose. The genes of the L-arabinose pathway, which were unknown, i.e. L-arabinitol 4-dehydrogenase and L-xylulose reductase, were identified. These genes, together with the known genes of the L-arabinose pathway, form a functional pathway. This pathway can be introduced to a fungus, which is completely or partially lacking this pathway.

    摘要翻译： 真菌微生物可以通过遗传工程进行工程化以利用L-阿拉伯糖。 鉴定了未知的L-阿拉伯糖途径的基因，即L-阿拉伯糖醇4-脱氢酶和L-木酮糖还原酶。 这些基因与已知的L-阿拉伯糖途径的基因一起形成功能途径。 该途径可以被引入真菌，其完全或部分缺乏该途径。

    MANUFACTURE OF XYLONIC ACID
    7.
    发明申请
    MANUFACTURE OF XYLONIC ACID 有权
    制备方法

    公开(公告)号：US20120005788A1

    公开(公告)日：2012-01-05

    申请号：US13256559

    申请日：2010-03-17

    申请人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

    发明人： Peter Richard ,  Marilyn Wiebe ,  Mervi Toivari ,  Dominik Mojzita ,  Laura Ruohonen ,  Merja Penttilä

    IPC分类号： A01H15/00 ,  C12N1/15 ,  C04B18/18 ,  C12P7/40

    CPC分类号： C12P7/58 ,  C12N9/0006

    摘要： Provided is a method for producing xylonic acid from xylose with a recombinant fungal strain that is genetically modified to express a xylose dehydrogenase gene, which is able to convert xylose to xylonolactone, which is spontaneously or enzymatically hydrolysed to xylonic acid. The xylonic acid is excreted outside the host cell. Xylonate production may be coupled with xylitol production. Alternatively, if xylitol production is not desired, its production is reduced by removing the aldose reductase (or specific xylose reductase) enzyme, which converts xylose to xylitol. Expression of a heterologous lactonase encoding gene may result in higher acid concentrations. The method is suitable for producing xylonic acid from a hemicellulose hydrolysate such as hydrolysed lignocellulosic plant biomass.

    摘要翻译： 本发明提供了一种从木糖中生产木糖的方法，该重组真菌菌株被遗传修饰以表达木糖脱氢酶基因，其能够将木糖转化为己内酰胺，该木糖自发或酶促水解成木糖酸。 木糖酸在宿主细胞外排出。 木糖苷生产可能与木糖醇生产相结合。 或者，如果不需要木糖醇生产，则通过除去将木糖转化为木糖醇的醛糖还原酶（或特异性木糖还原酶）酶来降低其产生。 异源内含子编码基因的表达可能导致较高的酸浓度。 该方法适用于从半纤维素水解产物如水解木质纤维素植物生物质中生产木糖酸。

    Method for decreasing the foam formation during cultivation of a microorganism
    8.
    发明授权
    Method for decreasing the foam formation during cultivation of a microorganism 失效
    在微生物培养期间减少泡沫形成的方法

    公开(公告)号：US07338779B1

    公开(公告)日：2008-03-04

    申请号：US10050000

    申请日：2000-08-21

    申请人： Tiina Nakari-Setälä ,  Merja Penttilä ,  Michael Bailey ,  Maija Tenkanen

    发明人： Tiina Nakari-Setälä ,  Merja Penttilä ,  Michael Bailey ,  Maija Tenkanen

    IPC分类号： C12P39/00

    CPC分类号： C07K14/37 ,  C07K2319/00 ,  C12N1/14

    摘要： This invention relates to a method for decreasing the foam formation during cultivation of a microorganism and to a method for producing an enhanced amount of a product of interest. The method comprises that the microorganism is modified in such a way that the microorganism does not produce an essential amount of at least one of the proteins, polypeptides or peptides associated with foam formation during cultivation of the unmodified microorganism. In particular the method comprises that the microorganism is modified not to produce an essential amount of amphipathic or hydrophobic proteins, polypeptides or peptides.

    摘要翻译： 本发明涉及一种降低微生物培养过程中泡沫形成的方法及其生产方法。 该方法包括微生物的修饰，使得微生物在培养未修饰的微生物期间不产生必需量的至少一种与泡沫形成相关的蛋白质，多肽或肽中的至少一种。 特别地，该方法包括修饰微生物不产生必需量的两亲性或疏水性蛋白质，多肽或肽。