公开(公告)号：US20140135198A1

公开(公告)日：2014-05-15

申请号：US14062763

申请日：2013-10-24

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Aditya RAJAGOPAL ,  Carl J. ALLENDORPH ,  Axel SCHERER

IPC: C12M1/00

CPC classification number: B04B5/0414 ,  B01L3/50215 ,  B04B5/10

Abstract: Methods and devices for molecular analysis are disclosed, based on centrifugation. A centrifuge device has centrifuge tubes and elements to create electric fields. The shear forces applied to the cells inside a solution with biological molecules permit the performance of different analytic techniques, such as lysis and sample preparation for PCR.

Abstract translation: 公开了基于离心的分子分析方法和装置。 离心机具有离心管和元件以产生电场。 用生物分子施加到溶液中的细胞的剪切力允许执行不同的分析技术，例如用于PCR的裂解和样品制备。

公开(公告)号：US20140072993A1

公开(公告)日：2014-03-13

申请号：US13852476

申请日：2013-03-28

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Aditya RAJAGOPAL ,  Axel SCHERER ,  Michael D. HENRY ,  Sameer WALAVALKAR ,  Thomas A. TOMBRELLO ,  Andrew P. HOMYK

IPC: G01N27/327

CPC classification number: C12Q1/02 ,  A61B5/04001 ,  G01N27/3275 ,  G01N27/4146 ,  G01R1/06711 ,  G01R1/07342 ,  H01L29/413 ,  H01L29/42376 ,  H01L29/4966 ,  H01L29/66477 ,  H01L29/78 ,  H01L29/7831

Abstract: The basic structure and functionality of a probe as disclosed herein allows for flexibly incorporating into the probe, various sensing elements for various sensing applications. Two example applications among these various sensing applications include bio-sensing and chemical-sensing applications. For bio-sensing applications the probe, which is fabricated upon a silicon substrate, includes a bio-sensing element such as a nano-pillar transistor, and for chemical-sensing applications the probe includes a sensing element that has a functionalized contact area whereby the sensing element generates a voltage when exposed to one or more chemicals of interest.

Abstract translation: 本文公开的探针的基本结构和功能允许灵活地并入到探针中，用于各种感测应用的各种感测元件。 这些各种感测应用中的两个示例应用包括生物感测和化学感测应用。 对于生物感测应用，制造在硅衬底上的探针包括诸如纳米柱晶体管的生物感测元件，并且对于化学感测应用，探针包括具有功能化接触区域的感测元件， 当暴露于感兴趣的一种或多种化学品时，感测元件产生电压。

公开(公告)号：US20130267005A1

公开(公告)日：2013-10-10

申请号：US13913321

申请日：2013-06-07

Applicant: UNIVERSITY OF SOUTHERN CALIFORNIA ,  CALIFORNIA INSTITUTE OF TECHNOLOGY ,  APIC CORPORATION

Inventor： Emil P. KARTALOV ,  Axel SCHERER ,  Koichi SAYANO

IPC: B01L3/00

CPC classification number: B01L3/502753 ,  A61M1/3472 ,  A61M1/3496 ,  A61M1/3633 ,  A61M2205/0244 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2400/0424 ,  Y10T436/25125 ,  Y10T436/25375

Abstract: A microfluidic fluid separator for separating target components of a fluid by filtration is described. Methods for separating target components of a fluid by filtration and methods for processing blood on a large scale with the microfluidic fluid separator are provided.

Abstract translation: 描述了用于通过过滤分离流体的目标成分的微流体流体分离器。 提供了通过过滤分离流体的目标组分的方法和用微流体分离器大规模处理血液的方法。

公开(公告)号：US20130183676A1

公开(公告)日：2013-07-18

申请号：US13740112

申请日：2013-01-11

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Samson CHEN ,  Aditya RAJAGOPAL ,  Axel SCHERER

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/6408

Abstract: A device and method are described in which the lifetime of a fluorescent species or fluorophores is detected in the absence of any optical filter. Based on the measured fluorescent lifetimes, molecules or compounds attached to a fluorophores such as small organic molecules, polymers, peptides, saccharides and nucleic acids can be identified or assayed.

Abstract translation: 描述了在没有任何滤光器的情况下检测荧光物质或荧光团的寿命的装置和方法。 基于测量的荧光寿命，可以鉴定或测定与荧光团如小有机分子，聚合物，肽，糖和核酸连接的分子或化合物。

公开(公告)号：US20240260865A1

公开(公告)日：2024-08-08

申请号：US18623971

申请日：2024-04-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Samson CHEN ,  Axel SCHERER ,  Dvin I. ADALIAN ,  Peter PETILLO ,  Muhammad Musab JILANI ,  Xiomara L. MADERO ,  Deepan KISHORE KUMAR

IPC: A61B5/1486 ,  A61B5/145 ,  A61B5/1459 ,  C12N9/02 ,  C12N11/02 ,  C12N11/14 ,  G01N27/327

CPC classification number: A61B5/14865 ,  A61B5/14532 ,  A61B5/1459 ,  C12N9/0004 ,  C12N11/02 ,  C12N11/14 ,  G01N27/3272 ,  C12Y101/01001 ,  C12Y101/03003 ,  C12Y101/03005 ,  C12Y101/03007 ,  C12Y101/03008 ,  C12Y101/0301 ,  C12Y101/03011 ,  C12Y101/03012 ,  C12Y101/03015 ,  C12Y101/03016 ,  C12Y101/03018 ,  C12Y101/03019 ,  C12Y101/0302 ,  C12Y101/03023 ,  C12Y101/03027 ,  C12Y101/03029 ,  C12Y101/0303 ,  C12Y101/03037 ,  C12Y101/03038 ,  C12Y101/0304 ,  C12Y101/03041

Abstract: A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

公开(公告)号：US20240201090A1

公开(公告)日：2024-06-20

申请号：US18592324

申请日：2024-02-29

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. MALIK ,  Xiomara Linnette MADERO ,  Erika F. GARCIA ,  Sheel Mukesh SHAH ,  Axel SCHERER

IPC: G01N21/64 ,  C12Q1/6851

CPC classification number: G01N21/6486 ,  C12Q1/6851

Abstract: Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.

公开(公告)号：US20240018573A1

公开(公告)日：2024-01-18

申请号：US18352112

申请日：2023-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER

IPC: C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/6486 ,  G06F17/10

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US20230121718A1

公开(公告)日：2023-04-20

申请号：US18046737

申请日：2022-10-14

Applicant: California Institute of Technology ,  Gottfried Wilhelm Leibniz Universität Hannover ,  Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)

Inventor： Oliver PLETTENBURG ,  Christin AHLBRECHT ,  Dvin ADALIAN ,  Axel SCHERER ,  Xiomara Linnette MADERO ,  Samson CHEN ,  Muhammad Musab JILANI

IPC: G01N27/327

Abstract: Methods and systems are described for fabricating thin hydrogel layers on biosensors by a drop-spin method, which includes placing a drop of the hydrogel on the electrode, spinning the wafer at high speed in a vacuum, and heating the wafer to cure. One and multilayer sensors can be fabricated in this way, by adding layers of hydrogel or metal.

公开(公告)号：US20230097858A1

公开(公告)日：2023-03-30

申请号：US17937178

申请日：2022-09-30

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Axel SCHERER

IPC: G01N27/04 ,  G01N27/22 ,  C12Q1/6869

Abstract: Systems and methods for identifying the components of a long-chain molecule by making electrical measurements from fabricated nanoscale electrodes as the molecule moves down a narrow microfluidic channel. The channel can be along the surface of a chip, through a chip, or both.

公开(公告)号：US20220289348A1

公开(公告)日：2022-09-15

申请号：US17582991

申请日：2022-01-24

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Axel SCHERER

IPC: B63C11/02 ,  G01N27/22 ,  G01N21/41

Abstract: Methods and devices including implantable micro-sensors used to detect tissue-dissolved inert gas and to detect microbubble formation to avoid Caisson disease are described. The disclosed methods and devices are based on measuring the refractive index changes in hydrophobic liquids after absorbing an inert gas such as nitrogen. The changes in the refractive index are based on implementing one of an interferometry, optical microcavity resonance shift, a photonic crystal resonance, a beam deflection, a resonance tuning or detuning, an amplitude change, or an intensity change method.