公开(公告)号：US20140057210A1

公开(公告)日：2014-02-27

申请号：US14070469

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. MALIK ,  Axel SCHERER ,  Erika F. GARCIA ,  Xiomara L. MADERO

IPC: B01L3/00

CPC classification number: B01L3/52 ,  B01L3/502707 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0689 ,  B01L2300/0654 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2400/0478 ,  B33Y80/00 ,  Y10T156/10

Abstract: Methods to fabricate reaction cartridges for biological sample preparation and analysis are disclosed. A cartridge may have a reaction chamber and openings to allow fluids to enter the chamber. The cartridge may also have handles to facilitate its use. Such cartridges may be used for polymerase chain reaction.

Abstract translation: 公开了制备用于生物样品制备和分析的反应柱的方法。 墨盒可以具有反应室和开口以允许流体进入腔室。 墨盒也可以具有便于使用的手柄。 这种药盒可用于聚合酶链反应。

公开(公告)号：US20180052110A1

公开(公告)日：2018-02-22

申请号：US15701014

申请日：2017-09-11

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. MALIK ,  Xiomara Linnette MADERO ,  Erika F. GARCIA ,  Sheel Mukesh SHAH ,  Axel SCHERER

IPC: G01N21/64 ,  C12Q1/68

CPC classification number: G01N21/6486 ,  C12Q1/6851 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2565/629

Abstract: Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.

公开(公告)号：US20140127790A1

公开(公告)日：2014-05-08

申请号：US14070454

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. MALIK ,  Erika F. GARCIA ,  Xiomara Linnette MADERO ,  Axel SCHERER

IPC: C12Q1/68

CPC classification number: C12M21/18 ,  B01L3/0275 ,  B01L3/502707 ,  B01L3/502715 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1827 ,  B01L2400/0478

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有容器的线性或圆形布置，其具有连接结构，例如杆或盘。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 可以将不同的功能元件，例如研磨机或混合器附接到容器。

公开(公告)号：US20140057279A1

公开(公告)日：2014-02-27

申请号：US14070466

申请日：2013-11-01

Applicant: California Institute Of Technology

Inventor： Imran R. MALIK ,  Erika F. GARCIA ,  Xiomara Linnette MADERO ,  Axel SCHERER

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01L3/502715 ,  B01L3/502723 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2400/0478 ,  F17D3/00 ,  G01N1/286 ,  G01N21/03 ,  G01N21/552 ,  G01N21/648 ,  G01N35/04 ,  G01N2001/2866 ,  G01N2001/4061 ,  G01N2021/0325 ,  G01N2035/00326 ,  G01N2035/00465 ,  G01N2035/0446 ,  G01N2035/1048 ,  G01N2035/1058 ,  G01N2201/0221 ,  Y02A50/58

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers. The device may have a reaction cartridge with a reaction chamber to perform techniques such as polymerase chain reaction.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有容器的线性或圆形布置，其具有连接结构，例如杆或盘。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 可以将不同的功能元件，例如研磨机或混合器附接到容器。 该装置可以具有带有反应室的反应筒，以进行诸如聚合酶链式反应的技术。

公开(公告)号：US20240167105A1

公开(公告)日：2024-05-23

申请号：US18419486

申请日：2024-01-22

Applicant: California Institute of Technology

Inventor： Aditya RAJAGOPAL ,  Mark D. GOLDBERG ,  Erika F. GARCIA ,  Xiomara L. MADERO ,  Thomas A. TOMBRELLO ,  Axel SCHERER

IPC: C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333

Abstract: A non-transitory computer-readable storage medium storing executable instructions to cause a system to detect a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a measured change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US20140127796A1

公开(公告)日：2014-05-08

申请号：US14070440

申请日：2013-11-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. MALIK ,  Axel SCHERER ,  Erika F. GARCIA ,  Xiomara L. MADERO

IPC: C12M1/40

CPC classification number: C12M21/18 ,  B01L3/0275 ,  B01L3/502707 ,  B01L3/502715 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/04 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  B01L2300/1827 ,  B01L2400/0478

Abstract: Methods and devices for biological sample preparation and analysis are disclosed. A device may have a linear or circular arrangement of containers, with a connecting structure such as a bar or disk. Fluidics channels between containers allow the performance of different techniques for sample preparation, such as lysing, washing and elution. Different functional elements, such as grinders or mixers, may be attached to the containers.

Abstract translation: 公开了用于生物样品制备和分析的方法和装置。 装置可以具有容器的线性或圆形布置，其具有连接结构，例如杆或盘。 容器之间的流体通道允许执行不同的样品制备技术，如裂解，洗涤和洗脱。 可以将不同的功能元件，例如研磨机或混合器附接到容器。

公开(公告)号：US20140036267A1

公开(公告)日：2014-02-06

申请号：US13947469

申请日：2013-07-22

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. MALIK ,  Xiomara L. MADERO ,  Erika F. GARCIA ,  Axel SCHERER

IPC: G01N21/64

CPC classification number: G01N21/64 ,  G01N21/0303 ,  G01N21/645

Abstract: Structures and methods are described for optical detection of physical, chemical and/or biological samples. An optical detection structure may include a LED source, multiple filters and single or multiple sample areas. A detector may be used to record a fluorescence signal. The sample area may allow the introduction of removable cartridges.

Abstract translation: 描述了物理，化学和/或生物样品的光学检测的结构和方法。 光学检测结构可以包括LED源，多个滤光器和单个或多个样品区域。 检测器可用于记录荧光信号。 样品区域可能允许引入可移除的墨盒。

公开(公告)号：US20240201090A1

公开(公告)日：2024-06-20

申请号：US18592324

申请日：2024-02-29

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. MALIK ,  Xiomara Linnette MADERO ,  Erika F. GARCIA ,  Sheel Mukesh SHAH ,  Axel SCHERER

IPC: G01N21/64 ,  C12Q1/6851

CPC classification number: G01N21/6486 ,  C12Q1/6851

Abstract: Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.

公开(公告)号：US20140213471A1

公开(公告)日：2014-07-31

申请号：US14162725

申请日：2014-01-23

Applicant: California Institute of Technology

Inventor： Aditya RAJAGOPAL ,  Mark D. GOLDBERG ,  Erika F. GARCIA ,  Xiomara L. MADERO ,  Thomas A. TOMBRELLO ,  Axel SCHERER

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.

Abstract translation: 本公开提供了用于检测样品中多种分析物的方法，组合物和试剂盒。 在一些实例中，本公开提供了用于检测分析物，遗传变异，监测反应过程以及通过测量信号监测分析物 - 分析物相互作用的方法，组合物和试剂盒。 在一些示例中，可以使用信号的存在或信号的改变来构建可用于检测分析物的信号谱。

公开(公告)号：US20140038195A1

公开(公告)日：2014-02-06

申请号：US13958479

申请日：2013-08-02

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Imran R. MALIK ,  Xiomara Linnette MADERO ,  Erika F. GARCIA ,  Sheel Mukesh SHAH ,  Axel SCHERER

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  C12Q1/6851 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2565/629

Abstract: Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.

Abstract translation: 提出了多路复用单检测通道扩增过程的方法和算法以及生成的扩增子的定量。 提出了用于量化和验证反应中扩增子的各种数学方法。 这种方法和方法的使用允许升级现有的单通道和多通道仪器以进一步复用能力。