公开(公告)号：US10961576B2

公开(公告)日：2021-03-30

申请号：US16707554

申请日：2019-12-09

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jeffrey G Mandell ,  Kevin L Gunderson ,  Jingwei Bai ,  Liangliang Qiang ,  Bradley Baas

IPC: C12Q1/6874 ,  G01N33/487 ,  G01N27/447

Abstract: The disclosure provides detection apparatus having one or more nanopores, methods for making apparatus having one or more nanopore and methods for using apparatus having one or more nanopores. Uses include, but are not limited to detection and sequencing of nucleic acids.

公开(公告)号：US20190283024A1

公开(公告)日：2019-09-19

申请号：US16433969

申请日：2019-06-06

Applicant: Illumina, Inc.

Inventor： Alex Aravanis ,  Boyan Boyanov ,  M. Shane Bowen ,  Dale Buermann ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Poorya Sabounchi ,  Hai Quang Tran

IPC: B01L3/00 ,  F16K99/00 ,  B01L7/00

Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

公开(公告)号：US10350570B2

公开(公告)日：2019-07-16

申请号：US15535695

申请日：2015-12-14

Applicant: Illumina, Inc.

Inventor： Kevin L. Gunderson ,  Jingwei Bai ,  Boyan Boyanov

IPC: C12Q1/68 ,  B01J19/00 ,  C12Q1/6837

Abstract: Provided herein are methods and compositions for placing single target molecules on a patterned substrate.

公开(公告)号：US20160356715A1

公开(公告)日：2016-12-08

申请号：US15175489

申请日：2014-12-09

Applicant: Illumina, Inc.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: G01N21/64 ,  G01N21/05

CPC classification number: G01N21/645 ,  B01L3/502707 ,  B01L3/502715 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/6454 ,  G01N33/54373 ,  G01N2021/6441 ,  G01N2021/6482 ,  G01N2201/061 ,  G01N2201/0642

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. The biosensor also includes a shield layer having apertures that are positioned relative to the input regions of corresponding light guides such that the light emissions propagate through the apertures into the corresponding input regions. The shield layer extends between adjacent apertures and is configured to block the excitation light and the light emissions incident on the shield layer between the adjacent apertures.

Abstract translation: 生物传感器包括具有光传感器的传感器阵列的装置基座和光导的引导阵列。 光导具有被配置为接收由生物或化学物质产生的激发光和发光的输入区域。 光导件延伸到装置底座中朝向相应的光传感器并具有过滤材料。 器件基底包括电耦合到光传感器并被配置为传输数据信号的器件电路。 生物传感器还包括具有相对于相应光导的输入区域定位的孔的屏蔽层，使得光发射通过孔径传播到相应的输入区域。 屏蔽层在相邻的孔之间延伸并且被配置为阻挡入射在相邻孔之间的屏蔽层上的激发光和发光。

公开(公告)号：US20250164399A1

公开(公告)日：2025-05-22

申请号：US19027132

申请日：2025-01-17

Applicant: Illumina, Inc.

Inventor： Tracy Helen Fung ,  Xiuyu Cai ,  Lisa Kwok ,  Hai Tran ,  Kevan Samiee ,  Liangliang Qiang ,  Boyan Boyanov

IPC: G01N21/64 ,  B01L3/00 ,  G01N15/1434 ,  G01N21/77

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US12179190B2

公开(公告)日：2024-12-31

申请号：US18087499

申请日：2022-12-22

Applicant: ILLUMINA, INC.

Inventor： Alex Aravanis ,  Boyan Boyanov ,  M. Shane Bowen ,  Dale Buermann ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Poorya Sabounchi ,  Hai Quang Tran

IPC: B01L3/00 ,  B01L7/00 ,  F16K99/00

Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

公开(公告)号：US20240293816A1

公开(公告)日：2024-09-05

申请号：US18574214

申请日：2022-06-30

Applicant: ILLUMINA, INC.

Inventor： Rean Silke Musa ,  Anthony Flannery ,  Boyan Boyanov ,  Nigel COBURN ,  Sharis MINASSIAN

IPC: B01L3/00 ,  C12Q1/6869

CPC classification number: B01L3/502761 ,  C12Q1/6869 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0893 ,  B01L2400/0427 ,  B01L2400/046

Abstract: Devices for sequencing biopolymers, methods of manufacturing the devices, and methods of using the devices are disclosed. In one example, such a device has a nanopore and a horizontal nanochannel. In some embodiments, the horizontal nanochannel may take a tortuous path. In some embodiments, such a device includes gas or air bubble generators or pressure pulse generators to block or unblock the horizontal nanochannel.

公开(公告)号：US20230332224A1

公开(公告)日：2023-10-19

申请号：US18317865

申请日：2023-05-15

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

CPC classification number: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0002

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US11680906B2

公开(公告)日：2023-06-20

申请号：US16607354

申请日：2018-04-19

Applicant: ILLUMINA, INC.

Inventor： Tracy Helen Fung ,  Xiuyu Cai ,  Lisa Kwok ,  Hai Tran ,  Kevan Samiee ,  Liangliang Qiang ,  Boyan Boyanov

IPC: G01N21/64 ,  G01N21/77 ,  B01L3/00 ,  G01N15/14

CPC classification number: G01N21/6454 ,  B01L3/502715 ,  G01N15/1436 ,  G01N21/6486 ,  G01N21/7703 ,  G01N2021/7763

Abstract: An example sensor includes a flow cell, a detection device, and a controller. The flow cell includes a passivation layer having opposed surfaces and a reaction site at a first of the opposed surfaces. The flow cell also includes a lid operatively connected to the passivation layer to partially define a flow channel between the lid and the reaction site. The detection device is in contact with a second of the opposed surfaces of the passivation layer, and includes an embedded metal layer that is electrically isolated from other detection circuitry of the detection device. The controller is to ground the embedded metal layer.

公开(公告)号：US20220299469A1

公开(公告)日：2022-09-22

申请号：US17828991

申请日：2022-05-31

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jens Gundlach

IPC: G01N27/414 ,  G01N33/487 ,  H01L21/74 ,  H01L29/66 ,  H01L29/786

Abstract: Example devices include a cis well associated with a cis electrode, a trans well associated with a trans electrode, and a field effect transistor (FET) positioned between the cis well and the trans well. Examples of the field effect transistor (FET) include a fluidic system defined therein. The fluidic system includes a first cavity facing the cis well, a second cavity fluidically connected to the trans well, and a through via extending through the field effect transistor from the first cavity. A first nanoscale opening fluidically connects the cis well and the first cavity, the first nanoscale opening having an inner diameter. A second nanoscale opening fluidically connects the through via and the second cavity, the second nanoscale opening having an inner diameter. The second nanoscale opening inner diameter is larger than the first nanoscale opening inner diameter.