公开(公告)号：US20150064785A1

公开(公告)日：2015-03-05

申请号：US14367413

申请日：2012-12-21

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Richard Fike ,  Shawn Barrett ,  Zhou Jiang ,  David Judd

IPC分类号： C12N5/00 ,  B29C67/24

CPC分类号： C12N5/005 ,  B01J13/06 ,  B01J13/22 ,  B29C67/24 ,  B29L2009/00 ,  C12N5/0018 ,  C12N2500/30 ,  C12N2500/38 ,  C12N2511/00 ,  C12N2533/40 ,  C12N2533/74

摘要： Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

摘要翻译： 描述了制备培养基，饲料和补品的组合物和方法。 这样的方法和介质可以显示不稳定组分的增加的稳定性，并且可以使用例如微悬浮和/或封装技术，螯合，以及任选地，将不稳定化合物与抗氧化剂包衣和/或混合。 组合物可以承受热和/或照射治疗并且具有减少的病毒数量。 这些技术可能导致产品具有延长的保质期，将其内部组分延长释放到培养物中，或可以无菌地添加到生物反应器中使用最小体积的产品中。 组合物和方法可以优化生物生产工作流程并提高效率。

公开(公告)号：US20150056647A1

公开(公告)日：2015-02-26

申请号：US14530251

申请日：2014-10-31

申请人： Vesta Therapeutics Inc.

发明人： Joseph Charles RUIZ ,  Steven Michael HOYNOWSKI

IPC分类号： C12N5/071

CPC分类号： C12N5/0676 ,  C12N5/067 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/90 ,  C12N2501/065 ,  C12N2506/02 ,  C12N2506/14 ,  C12N2509/00 ,  G01N33/5014 ,  G01N33/5038 ,  G01N33/507

摘要： The invention relates to methods for differentiating progenitor cells into insulin producing pancreatic islet cells and compositions and methods for using such cells.

摘要翻译： 本发明涉及将祖细胞分化成胰岛素产生胰岛细胞的方法以及使用这种细胞的组合物和方法。

公开(公告)号：US20150050733A1

公开(公告)日：2015-02-19

申请号：US14380938

申请日：2012-03-13

申请人： Guannan Su ,  Yannan Zhao ,  Jianshu Wei ,  Bing Chen ,  Zhifeng Xiao ,  Jianwu Dai

发明人： Guannan Su ,  Yannan Zhao ,  Jianshu Wei ,  Bing Chen ,  Zhifeng Xiao ,  Jianwu Dai

IPC分类号： C12N5/00

CPC分类号： C12N5/0062 ,  C12N5/0619 ,  C12N2500/30 ,  C12N2506/00 ,  C12N2533/76

摘要： Methods for reprogramming cells by culturing the cells under a condition that allows formation of a three-dimensional cell aggregate are provided. The cells and cell aggregates obtained using the methods are also provided.

摘要翻译： 提供了在允许形成三维细胞聚集体的条件下通过培养细胞来重编程细胞的方法。 还提供了使用这些方法获得的细胞和细胞聚集体。

公开(公告)号：US20140295482A1

公开(公告)日：2014-10-02

申请号：US14355890

申请日：2012-11-02

申请人： Texas Tech University System

发明人： Mark Lyte

IPC分类号： C12N1/20 ,  C12N1/14 ,  C12Q1/02 ,  C12N1/16

CPC分类号： C12N1/20 ,  C12N1/14 ,  C12N1/16 ,  C12N1/38 ,  C12N2500/30 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12Q1/025

摘要： Methods and compositions for enhancing or promoting germination of bacterial spores, and yeasts are disclosed herein. The composition of the present invention comprises an extract obtained from banana or any member belonging to the genus Musa that may be used alone or in a growth medium to promote and enhance germination of bacterial spores, growth of bacterial, yeast, and fungal cell cultures.

摘要翻译： 本文公开了用于增强或促进细菌孢子和酵母的发芽的方法和组合物。 本发明的组合物包括从香蕉或属于Musa属的任何成员获得的提取物，其可以单独使用或在生长培养基中使用以促进和增强细菌孢子的发芽，细菌，酵母和真菌细胞培养物的生长。

公开(公告)号：US20140234966A1

公开(公告)日：2014-08-21

申请号：US14130639

申请日：2012-07-05

申请人： Sandra Marie Merkel ,  Luke Dimasi ,  Collette Ann Sheahan ,  Philip Harvey Morton

发明人： Sandra Marie Merkel ,  Luke Dimasi ,  Collette Ann Sheahan ,  Philip Harvey Morton

IPC分类号： C12N5/0735 ,  C12N5/00

CPC分类号： C12N5/0018 ,  C07K14/435 ,  C07K14/76 ,  C12N5/0606 ,  C12N2500/30 ,  C12N2501/998

摘要： The invention relates to a new formulation of albumin, a method for producing the albumin formulation and to uses of the albumin formulation, for example in cell culture such as mammalian cell culture and particularly in stem cell culture.

摘要翻译： 本发明涉及白蛋白的新制剂，白蛋白制剂的制备方法和白蛋白制剂的用途，例如在细胞培养物如哺乳动物细胞培养物中，特别是在干细胞培养物中。

公开(公告)号：US08790662B2

公开(公告)日：2014-07-29

申请号：US13418263

申请日：2012-03-12

申请人： Brad J. Spellberg ,  Ashraf S. Ibrahim ,  John E Edwards, Jr.

发明人： Brad J. Spellberg ,  Ashraf S. Ibrahim ,  John E Edwards, Jr.

IPC分类号： A61K39/00 ,  A61K39/38 ,  A01N63/00 ,  A01N65/00 ,  A01N63/04

CPC分类号： C12N5/0694 ,  A61K2035/124 ,  C12N5/0642 ,  C12N2500/30 ,  C12N2500/44 ,  C12N2500/62 ,  C12N2501/385

摘要： The invention is based, in part, on the discovery of a novel cell-based immunotherapy that can recapitulate neutrophil functions in neutropenic individuals afflicted with a microbial infection. The therapeutic methods of the invention are broadly applicable to treat any infection in a neutropenic individual, including infections caused by bacteria, fungi, protozoa, and viruses. The methods of the invention represent a practical, rapid cell-based immunotherapy for refractory infections comprising compositions of activated, irradiated HL-60 cells.

摘要翻译： 本发明部分地基于发现一种新的基于细胞的免疫疗法，其可以概括地患有微生物感染的中性粒细胞减少的个体中的嗜中性粒细胞功能。 本发明的治疗方法广泛适用于治疗嗜中性粒细胞减少个体的任何感染，包括由细菌，真菌，原生动物和病毒引起的感染。 本发明的方法代表了用于难治性感染的实用的基于细胞的快速免疫治疗，其包含活化的照射的HL-60细胞的组合物。

公开(公告)号：US08747294B2

公开(公告)日：2014-06-10

申请号：US12740800

申请日：2008-10-31

申请人： Christopher William Lipari ,  Annabelle Rodriguez

发明人： Christopher William Lipari ,  Annabelle Rodriguez

IPC分类号： A61B17/43 ,  A61D7/00 ,  A61B17/435

CPC分类号： A61B17/435 ,  C12N5/0604 ,  C12N2500/30 ,  G01N33/84 ,  G01N2800/367

摘要： The present invention relates, e.g., to a method for predicting the embryonic progression of a preimplantation embryo, comprising measuring in the insemination medium (IM) in which an oocyte was fertilized and subsequently incubated in vitro, thereby generating a pre-implantation embryo, at 18-24 hrs post fertilization, the levels of nitric oxide metabolites (NOx) in the insemination medium (IM), wherein a level of NOx of at least about 1.3 μM indicates that the preimplantation embryo is likely to progress to the blastocyst stage by culture day (CD) 5.

摘要翻译： 本发明涉及例如用于预测植入前胚胎的胚胎进展的方法，包括在受精培养基（IM）中测量卵母细胞受精并随后在体外温育，从而产生植入前胚胎， 受精后18-24小时，授精培养基（IM）中一氧化氮代谢物（NOx）的水平，其中至少约1.3μM的NOx水平表明植入前胚胎可能通过培养进入囊胚阶段 天（CD）5。

公开(公告)号：US20140106343A1

公开(公告)日：2014-04-17

申请号：US14010578

申请日：2013-08-27

申请人： Asterias Biotherapeutics, Inc.

发明人： Ramkumar Mandalam ,  Saadia Faouzi ,  Isabelle Nadeau ,  Kristina Pfendler-Bonham ,  Namitha Rao ,  Melissa K. Carpenter ,  Lakshmi Rambhatla ,  Choy-Pik Chiu

IPC分类号： C12N5/071

CPC分类号： C12N5/067 ,  C12N2500/30 ,  C12N2500/36 ,  C12N2500/62 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/13 ,  C12N2501/148 ,  C12N2501/155 ,  C12N2501/23 ,  C12N2501/33 ,  C12N2501/335 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2502/13 ,  C12N2503/02 ,  C12N2506/02 ,  C12N2533/90 ,  C12N2533/92

摘要： This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy.

摘要翻译： 本公开提供了一种新开发的策略和将多能干细胞分化成肝细胞谱系细胞的特定选择。 许多方案都是基于这样的策略，其中细胞首先分化成早期胚层细胞，然后分化成肝细胞前体，然后进入成熟细胞。 获得的细胞具有形态学特征和人成人肝细胞特征的表型标志物。 它们还显示细胞色素p450酶活性的证据，验证其在商业应用中的用途，例如药物筛选，或用于制备用于临床治疗的药物和医疗装置。

公开(公告)号：US20130323837A1

公开(公告)日：2013-12-05

申请号：US13848587

申请日：2013-03-21

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： David JUDD ,  Paul Battista

IPC分类号： C12N5/071

CPC分类号： C12N5/0629 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/113

摘要： The present invention provides cell culture media formulations which support the in vitro cultivation of animal epithelial cells. The media comprise at least one fibroblast growth factor (FGF) and at least one agent that induces increased intracellular cAMP levels, and optionally comprise ascorbic acid. The present invention also provides methods of cultivating animal epithelial cells in vitro using these cell culture media formulations, kits comprising the media, cell culture compositions comprising the culture media and an animal epithelial cell, and compositions that may be used as replacements for organ or gland extracts in animal cell culture media.

摘要翻译： 本发明提供支持体外培养动物上皮细胞的细胞培养基制剂。 培养基包含至少一种成纤维细胞生长因子（FGF）和至少一种诱导增加的细胞内cAMP水平并且任选地包含抗坏血酸的试剂。 本发明还提供了使用这些细胞培养基制剂在体外培养动物上皮细胞的方法，包含培养基的试剂盒，包含培养基和动物上皮细胞的细胞培养组合物，以及可用作器官或腺体的替代物的组合物 提取物在动物细胞培养基中。

公开(公告)号：US20130316400A1

公开(公告)日：2013-11-28

申请号：US13886226

申请日：2013-05-02

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Sanjay VASU ,  Henry CHIOU ,  Jeffrey ROGERS ,  Maria Isabel CISNEROS ,  Jingqiu LI ,  Chao Yan LIU ,  Meredith JONES

IPC分类号： C07K14/505

CPC分类号： C07K14/505 ,  C12N5/005 ,  C12N5/0619 ,  C12N2500/30 ,  C12N2501/065 ,  C12N2501/999 ,  C12N2510/02 ,  C12N2511/00

摘要： The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

摘要翻译： 本发明一般涉及可用于将大分子和化合物（例如核酸分子）引入细胞（例如真核细胞）中的细胞培养基（特别是无血清，非动物衍生的和/或化学上限定的培养基）。 根据本发明，这种引入可以在所述介质的存在下进行。 然后可以在培养基中培养含有这种引入的物质的细胞，并且可以测量或测定引入的细胞对细胞的影响。 特别地，本发明允许将核酸分子（例如，载体）引入细胞（特别是真核细胞）中，以及由细胞中核酸分子编码的蛋白质的表达。 本发明消除了在每次用细胞（例如，培养细胞与转染细胞）进行不同程序时改变细胞培养基的需要。 本发明还涉及可用于培养和转化/转染细胞的组合物和试剂盒。