公开(公告)号：US20250073710A1

公开(公告)日：2025-03-06

申请号：US18951464

申请日：2024-11-18

Applicant: ILLUMINA, INC.

Inventor： Alex Aravanis ,  Boyan Boyanov ,  M. Shane Bowen ,  Dale Buermann ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Poorya Sabounchi ,  Hai Quang Tran

IPC: B01L3/00 ,  B01L7/00 ,  F16K99/00

Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

公开(公告)号：US20240377380A1

公开(公告)日：2024-11-14

申请号：US18553192

申请日：2022-03-01

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Jeffrey G. Mandell ,  Seth M. McDonald

IPC: G01N33/487

Abstract: An example of a nanopore sensor device includes one or more cis wells; a cis electrode; a plurality of trans wells, each of the plurality of trans wells separated from the one or more cis wells by a lipid/solid-state membrane having a nanopore; a plurality of trans electrodes, each of the plurality of trans electrodes associated with one of the plurality of trans wells; a first concentration of an electrolyte within the one or more cis wells; and a second concentration of the electrolyte within the trans wells, wherein the first concentration is higher than the second concentration.

公开(公告)号：US20240280557A1

公开(公告)日：2024-08-22

申请号：US18552532

申请日：2022-03-10

Applicant: Illumina, Inc.

Inventor： Lisa Savagian ,  Burton Simpson ,  Sang Park ,  Boyan Boyanov ,  Jeffrey G. Mandell ,  Seth M. McDonald

IPC: G01N33/487 ,  B82B1/00 ,  B82B3/00 ,  C12Q1/6869

CPC classification number: G01N33/48721 ,  B82B1/008 ,  B82B3/0038 ,  C12Q1/6869

Abstract: Polypeptide nanopores synthetically functionalized with positively charged species, and methods of making and using the same, are provided herein. In some examples, a polypeptide nanopore includes a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue. The mutated amino acid residue may be synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel.

公开(公告)号：US20240207842A1

公开(公告)日：2024-06-27

申请号：US18542511

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Gianluca Andrea Artioli ,  Johan Sebastian Basuki ,  Boyan Boyanov ,  Gabriele Canzi ,  Sahngki Hong ,  Nam Nguyen ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov ,  Xavier von Hatten

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L2200/025 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/1805

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a gas-dissolvable coating.

公开(公告)号：US11898983B2

公开(公告)日：2024-02-13

申请号：US18003493

申请日：2021-06-18

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Rico Otto ,  Jeffrey G. Mandell

IPC: G01N27/414

CPC classification number: G01N27/4146

Abstract: Devices and methods of using the devices are disclosed which can provide scalability, improved sensitivity and reduced noise for sequencing polynucleotide. Examples of the devices include a biological or solid-state nanopore, a field effect transistor (FET) sensor with improved gate controllability over the channel, and a porous structure.

公开(公告)号：US11774400B2

公开(公告)日：2023-10-03

申请号：US17828991

申请日：2022-05-31

Applicant: Illumina, Inc.

Inventor： Boyan Boyanov ,  Jens Gundlach

IPC: G01N27/414 ,  G01N33/487 ,  H01L21/74 ,  H01L29/66 ,  H01L29/786

CPC classification number: G01N27/4145 ,  G01N27/4146 ,  G01N33/48721 ,  H01L21/743 ,  H01L29/66772 ,  H01L29/78696

Abstract: Example devices include a cis well associated with a cis electrode, a trans well associated with a trans electrode, and a field effect transistor (FET) positioned between the cis well and the trans well. Examples of the field effect transistor (FET) include a fluidic system defined therein. The fluidic system includes a first cavity facing the cis well, a second cavity fluidically connected to the trans well, and a through via extending through the field effect transistor from the first cavity. A first nanoscale opening fluidically connects the cis well and the first cavity, the first nanoscale opening having an inner diameter. A second nanoscale opening fluidically connects the through via and the second cavity, the second nanoscale opening having an inner diameter. The second nanoscale opening inner diameter is larger than the first nanoscale opening inner diameter.

公开(公告)号：US20230272457A1

公开(公告)日：2023-08-31

申请号：US18179026

申请日：2023-03-06

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Liangliang Qiang ,  Kevin L. Gunderson ,  Kay Klausing ,  Lea Pickering ,  Cyril Delattre ,  Tarun Khurana

IPC: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816

CPC classification number: C12Q1/6818 ,  C12Q1/6874 ,  C12Q1/6816 ,  C12Q2533/101 ,  C12Q2535/113 ,  C12Q2535/122 ,  C12Q2563/103 ,  C12Q2563/125

Abstract: Under one aspect, a composition includes a substrate; a first polynucleotide coupled to the substrate; a second polynucleotide hybridized to the first polynucleotide; and a catalyst coupled to a first nucleotide of the second polynucleotide, the catalyst being operable to cause a chemiluminogenic molecule to emit a photon. Under another aspect, a method includes providing a catalyst operable to cause a first chemiluminogenic molecule to emit a photon; providing a substrate; providing a first polynucleotide coupled to the substrate; hybridizing a second polynucleotide to the first polynucleotide; coupling a first quencher to a first nucleotide of the second polynucleotide; and inhibiting, by the first quencher, photon emission by the first chemiluminogenic molecule.

公开(公告)号：US11590494B2

公开(公告)日：2023-02-28

申请号：US16433969

申请日：2019-06-06

Applicant: Illumina, inc.

Inventor： Alex Aravanis ,  Boyan Boyanov ,  M. Shane Bowen ,  Dale Buermann ,  Alexander Hsiao ,  Behnam Javanmardi ,  Tarun Khurana ,  Poorya Sabounchi ,  Hai Quang Tran

IPC: B01L3/00 ,  F16K99/00 ,  B01L7/00

Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

公开(公告)号：US11567060B2

公开(公告)日：2023-01-31

申请号：US16624567

申请日：2018-06-19

Applicant: ILLUMINA, INC.

Inventor： Boyan Boyanov ,  Rohan N. Akolkar ,  Jeffrey S. Fisher ,  Jeffrey G. Mandell ,  Liangliang Qiang ,  Steven M. Barnard

IPC: G01N33/487 ,  C12Q1/6869 ,  G01N27/447

Abstract: Example nanopore sequencers include a cis well, a trans well, and a nanopore fluidically connecting the cis and trans wells. In one example sequencer, a modified electrolyte (including an electrolyte and a cation complexing agent) is present in the cis well, or the trans well, or in the cis and the trans wells. In another example sequencer, a gel state polyelectrolyte is present in the cis well, or the trans well, or in the cis and the trans wells.

公开(公告)号：US11181478B2

公开(公告)日：2021-11-23

申请号：US16295973

申请日：2019-03-07

Applicant: Illumina, Inc.

Inventor： Cheng Frank Zhong ,  Hod Finkelstein ,  Boyan Boyanov ,  Dietrich Dehlinger ,  Darren Segale

IPC: H01L27/146 ,  H01L27/00 ,  G01N27/414 ,  G01N33/543 ,  G01N21/64 ,  B01L3/00 ,  H01L27/088 ,  C12Q1/6874 ,  H01L29/78 ,  G01N21/05

Abstract: Biosensor including a device base having a sensor array of light sensors and a guide array of light guides. The light guides have input regions that are configured to receive excitation light and light emissions generated by biological or chemical substances. The light guides extend into the device base toward corresponding light sensors and have a filter material. The device base includes device circuitry electrically coupled to the light sensors and configured to transmit data signals. A passivation layer extends over the device base and forms an array of reaction recesses above the light guides. The biosensor also includes peripheral crosstalk shields that at least partially surround corresponding light guides of the guide array to reduce optical crosstalk between adjacent light sensors.