公开(公告)号：US20240207842A1

公开(公告)日：2024-06-27

申请号：US18542511

申请日：2023-12-15

Applicant: ILLUMINA, INC.

Inventor： Gianluca Andrea Artioli ,  Johan Sebastian Basuki ,  Boyan Boyanov ,  Gabriele Canzi ,  Sahngki Hong ,  Nam Nguyen ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov ,  Xavier von Hatten

IPC: B01L3/00

CPC classification number: B01L3/5027 ,  B01L2200/025 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/1805

Abstract: An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a gas-dissolvable coating.

公开(公告)号：US20250115957A1

公开(公告)日：2025-04-10

申请号：US18897718

申请日：2024-09-26

Applicant: Illumina, Inc.

Inventor： Claudia Battistella ,  Gabriele Canzi ,  Katharina Mueller-Ott ,  Wayne George ,  Jonathan Boutell

IPC: C12Q1/6874

Abstract: Some examples herein provide a sequencing flowcell that includes an imaging sensor; a hydrogel disposed on the imaging sensor and comprising a moiety; and a first complex non-covalently coupled to the moiety, the first complex comprising a first oligonucleotide. A method of using the sequencing flowcell may include decoupling the first complex from the moiety; and coupling a second complex to the moiety, the second complex comprising a second oligonucleotide. Methods of forming the flowcell are also provided.

公开(公告)号：US20250163491A1

公开(公告)日：2025-05-22

申请号：US18568655

申请日：2022-12-15

Applicant: Illumina, Inc.

Inventor： Gabriele Canzi

IPC: C12Q1/6806 ,  B01J23/44 ,  B01J23/75 ,  C12Q1/6869 ,  C12Q1/6876

Abstract: Embodiments of the present disclosure relate to method of chemical linearization of double stranded polynucleotides for sequencing by synthesis. In particular, a heterogenous cobalt catalyst is used to cleave one or more diol moieties at a predetermined cleavage site of one strand of the double stranded polynucleotides.