公开(公告)号：US20170267716A1

公开(公告)日：2017-09-21

申请号：US15071210

申请日：2016-03-16

Applicant: General Electric Company

Inventor： Anthony John Murray ,  Andrew David Pris ,  Erik Leeming Kvam ,  Nandini Nagraj ,  Eugene Pauling Boden ,  Ernest William Kovacs ,  Louisa Ruth Carr ,  Rui Chen ,  Tiberiu Mircea Siclovan ,  Kelly Scott Chichak

IPC: C07K1/36 ,  C07K16/00 ,  C12N9/74 ,  C12N15/115 ,  C07K14/705

CPC classification number: C07K1/36 ,  C07K1/32 ,  C07K14/70564 ,  C07K16/4291 ,  C12N9/6429 ,  C12Y304/21005

Abstract: Provided herein are aptamer-polymer conjugates which are responsive to environmental stimuli and are useful in selective purification of untagged target polypeptides.

公开(公告)号：US20170021333A1

公开(公告)日：2017-01-26

申请号：US15285986

申请日：2016-10-05

Applicant: General Electric Company

Inventor： Ernest William Kovacs ,  Frank John Mondello ,  Erik Leeming Kvam ,  Bing LI

IPC: B01J20/28 ,  C12N9/38 ,  C07K14/775 ,  C07K1/16 ,  C07K14/54 ,  C07K14/525 ,  C07K1/14 ,  B01J20/24 ,  C07K14/545

CPC classification number: B01J20/28038 ,  B01J20/22 ,  B01J20/24 ,  B01J20/28033 ,  B01J20/3212 ,  B01J20/3242 ,  B01J20/3246 ,  C07K1/145 ,  C07K1/16 ,  C07K14/525 ,  C07K14/5421 ,  C07K14/545 ,  C07K14/775 ,  C12N9/2471 ,  C12N15/1006 ,  C12N15/1017 ,  C12Y302/01023

Abstract: A solid substrate for the extraction, stabilization, and storage of proteins is provided. The substrate includes: a polysaccharide, such as melezitose under a substantially dry state. The substrate is configured to extract proteins from a sample and stabilize the extracted proteins in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the proteins stored in the dry solid substrate are also described.

Abstract translation: 提供了用于提取，稳定和储存蛋白质的固体底物。 底物包括：多糖，例如基本干燥状态下的松三草。 底物被配置为从样品中提取蛋白质并在环境条件下以干燥形式稳定所提取的蛋白质较长时间。 还描述了收集和回收储存在干固体基质中的蛋白质的方法。

公开(公告)号：US20160002621A1

公开(公告)日：2016-01-07

申请号：US14321160

申请日：2014-07-01

Applicant: General Electric Company

Inventor： John Richard Nelson ,  David Roger Moore ,  Robert Scott Duthie ,  Matthew Jeremiah Misner ,  Gregory Andrew Grossmann ,  Elizabeth Marie Dees ,  Patrick McCoy Spooner ,  Erik Leeming Kvam ,  Andrew Arthur Paul Burns ,  Vicki Herzl Watkins

IPC: C12N15/10

CPC classification number: C12N15/1006 ,  C12Q1/6834 ,  C12Q2565/625

Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.

Abstract translation: 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 并使核酸扩增反应混合物通过样品施用区跨越一定长度的底物扩增形成核酸扩增产物的靶核酸; 其中具有第一分子量的靶核酸基本上固定在样品施用区，并且其中具有第二分子量的扩增产物从样品施用区迁移。 还提供了相关联的设备。

公开(公告)号：US20150337263A1

公开(公告)日：2015-11-26

申请号：US14813406

申请日：2015-07-30

Applicant: General Electric Company

Inventor： Zaeem Ashraf Khan ,  Aaron Joseph Dulgar-Tulloch ,  Stefan Rakuff ,  Philip Alexander Shoemaker ,  Erik Leeming Kvam ,  Xiaohui Chen ,  Jaydeep Roy

IPC: C12N5/077

CPC classification number: C12M45/09 ,  B01F5/0057 ,  B01F5/0206 ,  B01F5/0231 ,  B01F5/0256 ,  B01F5/10 ,  C12M27/00 ,  C12M47/04 ,  C12N5/0653 ,  C12N2501/70 ,  C12N2501/734 ,  C12N2521/00

Abstract: A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer, introducing the adipose tissue into the vessel, introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided.

Abstract translation: 提供了一种处理脂肪组织以收集脂肪来源的再生细胞的方法，其中所述方法包括提供包含流体喷射混合器的容器，将脂肪组织引入容器中，将缓冲溶液引入容器中; 使用流体喷射混合器洗涤脂肪组织; 将酶溶液引入容器中; 使用流体喷射混合器将包含脂肪组织，酶溶液和缓冲溶液的射流混合到消化脂肪组织以形成消化产物; 将消化产物相分离成消化的浮力脂肪层和非浮力水层; 并收集包含脂肪来源的再生细胞的非浮力水层。 还提供了一种处理脂肪组织以收集脂肪来源的再生细胞的系统。

公开(公告)号：US09040679B2

公开(公告)日：2015-05-26

申请号：US13968497

申请日：2013-08-16

Applicant: General Electric Company

Inventor： Erik Leeming Kvam ,  Bing Li ,  Brian Christopher Bales

IPC: C12N15/10 ,  B01J20/24

CPC classification number: B01J20/24 ,  C12N15/1003 ,  C12N15/1006

Abstract: A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

Abstract translation: 提供了用于提取，稳定和储存核酸的固体基质。 至少一种蛋白质变性剂和至少一种酸或酸滴定的缓冲剂在基质中以干燥状态浸渍; 并且所述基质配置成在水合时提供酸性pH。 该基质构造成从样品中提取核酸，并在环境条件下长时间地以干燥形式稳定所提取的核酸，特别是RNA。 还描述了收集和回收存储在干燥固体基质中的核酸的方法。

公开(公告)号：US08981077B2

公开(公告)日：2015-03-17

申请号：US13968497

申请日：2013-08-16

Applicant: General Electric Company

Inventor： Erik Leeming Kvam ,  Bing Li ,  Brian Christopher Bales

IPC: C12N15/10 ,  B01J20/24

Abstract: A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

公开(公告)号：US20150031086A1

公开(公告)日：2015-01-29

申请号：US13952040

申请日：2013-07-26

Applicant: General Electric Company

Inventor： Ryan Charles Heller ,  John Richard Nelson ,  Erik Leeming Kvam

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12Q1/6869 ,  C12Q2521/501 ,  C12Q2525/307 ,  C12Q2527/137

Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of circulating nucleic acids extracted from blood is provided. Kits for performing the disclosed methods are also provided.

Abstract translation: 本文提供了在单个反应容器中从线性DNA产生和扩增单链DNA环的方法，而没有任何介入的纯化步骤。 通过不依赖模板的单链DNA连接产生单链DNA环。 提供了从血液中提取的循环核酸的全基因组扩增。 还提供了用于执行所公开的方法的套件。

公开(公告)号：US20130338351A1

公开(公告)日：2013-12-19

申请号：US13968497

申请日：2013-08-16

Applicant: General Electric Company

Inventor： Erik Leeming Kvam ,  Bing Li ,  Brian Christopher Bales

IPC: B01J20/24 ,  C12N15/10

CPC classification number: B01J20/24 ,  C12N15/1003 ,  C12N15/1006

Abstract: A solid matrix for the extraction, stabilization, and storage of nucleic acids is provided. At least one protein denaturant, and at least one acid or acid-titrated buffer reagent are impregnated in a dry state therein the matrix; and the matrix is configured to provide an acidic pH on hydration. The matrix is configured to extract nucleic acids from a sample and stabilize the extracted nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

Abstract translation: 提供了用于提取，稳定和储存核酸的固体基质。 至少一种蛋白质变性剂和至少一种酸或酸滴定的缓冲剂在基质中以干燥状态浸渍; 并且所述基质配置成在水合时提供酸性pH。 该基质构造成从样品中提取核酸，并在环境条件下长时间地以干燥形式稳定所提取的核酸，特别是RNA。 还描述了收集和回收存储在干燥固体基质中的核酸的方法。