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    Methods for spectrally resolving fluorophores of a sample and systems for same
    44.
    发明授权
    Methods for spectrally resolving fluorophores of a sample and systems for same 有权

    公开(公告)号：US12038326B2

    公开(公告)日：2024-07-16

    申请号：US17975243

    申请日：2022-10-27

    Applicant: Becton, Dickinson and Company

    Inventor： Peter Mage ,  Keegan Owsley

    IPC: G01J3/44 ,  G01N21/64

    CPC classification number: G01J3/4406 ,  G01N21/6428 ,  G01N21/6486

    Abstract: Aspects of the present disclosure include methods for spectrally resolving light from fluorophores having overlapping fluorescence spectra in a sample. Methods according to certain embodiments include detecting light with a light detection system from a sample having a plurality of fluorophores having overlapping fluorescence spectra and spectrally resolving light from each fluorophore in the sample. In some embodiments, methods include estimating the abundance of one or more of the fluorophores in the sample, such as on a particle. In certain instances, methods include identifying the particle in the sample based on the abundance of each fluorophore and sorting the particle. Methods according to some embodiments includes spectrally resolving the light from each fluorophore by calculating a spectral unmixing matrix for the fluorescence spectra of each fluorophore. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also provided.

    REAL-TIME ADAPTIVE METHODS FOR SPECTRALLY RESOLVING FLUOROPHORES OF A SAMPLE AND SYSTEMS FOR SAME
    45.
    发明公开
    REAL-TIME ADAPTIVE METHODS FOR SPECTRALLY RESOLVING FLUOROPHORES OF A SAMPLE AND SYSTEMS FOR SAME 审中-公开

    公开(公告)号：US20240192122A1

    公开(公告)日：2024-06-13

    申请号：US18537103

    申请日：2023-12-12

    Applicant: Becton, Dickinson and Company

    Inventor： Peter Mage ,  Keegan Owsley ,  Wenyu Bai

    IPC: G01N15/14 ,  G01N15/10 ,  G01N15/1429

    CPC classification number: G01N15/1459 ,  G01N15/1429 ,  G01N2015/1006

    Abstract: Aspects of the present disclosure include methods for spectrally resolving light from fluorophores having overlapping fluorescence spectra in a sample using adaptive measurement variance. Methods according to certain embodiments include detecting light with a light detection system from particles of a sample comprising a plurality of fluorophores having overlapping fluorescence spectra, determining measurement variance in the detected light for each particle and spectrally resolving light from each fluorophore in the sample with a weighted least squares algorithm that uses the measurement variance determined for each particle. In some embodiments, the spectral unmixing matrix is calculated by the weighted least squares algorithm using an adaptive measurement variance model. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also described. Non-transitory computer readable storage medium are also provided.

    Phase-Correction Of Radiofrequency-Multiplexed Signals
    46.
    发明公开
    Phase-Correction Of Radiofrequency-Multiplexed Signals 审中-公开

    公开(公告)号：US20240019354A1

    公开(公告)日：2024-01-18

    申请号：US18373487

    申请日：2023-09-27

    Applicant: Becton, Dickinson and Company

    Inventor： Jonathan Lin ,  Keegan Owsley ,  Matthew Bahr

    IPC: G01N15/14

    CPC classification number: G01N15/1459 ,  G01N15/1434 ,  G01N15/1425 ,  G01N15/1429 ,  G01N2015/1486 ,  G01N2015/1402 ,  G01N2015/142 ,  G01N2015/149

    Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

    Cell Sorting Using A High Throughput Fluorescence Flow Cytometer
    50.
    发明申请
    Cell Sorting Using A High Throughput Fluorescence Flow Cytometer 有权

    公开(公告)号：US20230076378A1

    公开(公告)日：2023-03-09

    申请号：US17953976

    申请日：2022-09-27

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64

    Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

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