公开(公告)号：US20180179556A1

公开(公告)日：2018-06-28

申请号：US15598316

申请日：2017-05-18

申请人： National Tsing Hua University ,  Chang Chun Plastics Co., Ltd. ,  Chang Chun Petrochemical Co., Ltd.

发明人： Roa-Pu Shen ,  Rex C. Wen

IPC分类号： C12P7/16 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12N9/10 ,  C12N15/70 ,  C12N15/52

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1217 ,  C12N15/52 ,  C12N15/70 ,  C12N2800/101 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y101/0103 ,  C12Y101/01322 ,  C12Y102/01004 ,  C12Y103/01086 ,  C12Y103/05004 ,  C12Y203/01009 ,  C12Y207/02001

摘要： A butanol expression cassette includes a butanol production related genes and a fermentation regulatory element. The fermentation regulatory element controls the expression of the butanol production related gene and locates upstream of the butanol production related gene. The fermentation regulatory element includes a promoter, a ribosome binding site and a transcription factor binding site of a fermentation gene. A fermentation in which the fermentation regulatory element involves includes an acetic acid fermentation, an alcohol fermentation, a succinic acid fermentation or a lactic acid fermentation, the butanol production related gene is not the fermentation gene or a gene of an upstream product of the fermentation in which the fermentation gene involves. The present invention provides a recombinant plasmid formed by cloning the butanol expression cassettes in the expression vector. The present invention also provides a butanol production related gene expression method to express butanol production related gene by using recombinant plasmid.

公开(公告)号：US20170101631A1

公开(公告)日：2017-04-13

申请号：US15293191

申请日：2016-10-13

申请人： LanzaTech New Zealand Limited

发明人： Michael Koepke ,  Rasmus Overgaard Jensen ,  James Bruce Yarnton Haycock Behrendorff ,  Ryan Edward Hill

IPC分类号： C12N9/12 ,  C12N9/02 ,  C12P5/00 ,  C12P7/04 ,  C12P7/42 ,  C12N9/10 ,  C12P7/28

CPC分类号： C12P7/42 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/16 ,  C12P5/007 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/28 ,  C12P7/625 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y102/07005 ,  C12Y203/01008 ,  C12Y203/01009 ,  C12Y203/01019 ,  C12Y207/02001 ,  C12Y207/02007 ,  C12Y301/0202

摘要： The invention relates to a genetically engineered bacterium comprising an energy-generating fermentation pathway and methods related thereto. In particular, the invention provides a bacterium comprising a phosphate butyryltransferase (Ptb) and a butyrate kinase (Buk) (Ptb-Buk) that act on non-native substrates to produce a wide variety of products and intermediates. In certain embodiments, the invention relates to the introduction of Ptb-Buk into a C1-fixing microoorgansim capable of producing products from a gaseous substrate.

公开(公告)号：US20160032323A1

公开(公告)日：2016-02-04

申请号：US14806343

申请日：2015-07-22

申请人： Danisco US Inc. ,  The Goodyear Tire & Rubber Company

发明人： Zachary Q. Beck ,  Michael C. Miller ,  Caroline M. Peres ,  Yuliya A. Primak ,  Jeff P. Pucci ,  Derek H. Wells

IPC分类号： C12P5/00 ,  C12P5/02 ,  C12N15/70

CPC分类号： C12P5/007 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/18 ,  C12N9/88 ,  C12N15/70 ,  C12P5/002 ,  C12P5/026 ,  C12P7/02 ,  C12P7/04 ,  C12P7/42 ,  C12Y101/01027 ,  C12Y101/01034 ,  C12Y101/01037 ,  C12Y102/01051 ,  C12Y102/04001 ,  C12Y203/01008 ,  C12Y203/01016 ,  C12Y203/03001 ,  C12Y203/0301 ,  C12Y207/02001 ,  C12Y301/01031 ,  C12Y401/01031 ,  Y02E50/343

摘要： The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).

摘要翻译： 本发明的特征在于通过在以下酶促途径中通过工程化微生物来增加碳通量以产生甲羟戊酸来生产甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯的组合物和方法：（a）柠檬酸合酶 ）磷酸转乙酰酶，（c）乙酸激酶，（d）乳酸脱氢酶，（e）苹果酸酶和（f）丙酮酸脱氢酶，使得其中一种酶活性被调节。 此外，通过mvaE和mvaS基因的异源表达（例如但不限于来自生物体李斯特氏菌DSM的mvaE和mvaS基因）可以进一步增强甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯的生产 20601，屎肠球菌，，肠球菌EG2，和黑麦草肠球菌）。

公开(公告)号：US20150247174A1

公开(公告)日：2015-09-03

申请号：US14395187

申请日：2012-12-28

申请人： ANHUI HUAHENG BIOENGINEERING CO., LTD.

发明人： Xueli Zhang ,  Dongzhu Zhang

IPC分类号： C12P13/06 ,  C12N15/70 ,  C12N9/90 ,  C12R1/19 ,  C12N9/06

CPC分类号： C12P13/06 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12R1/19 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y103/05001 ,  C12Y104/01001 ,  C12Y203/01054 ,  C12Y207/02001 ,  C12Y402/03003 ,  C12Y501/01001

摘要： The present invention discloses a DL-alanine-producing engineering bacterium. This DL-alanine-producing engineering bacterium itself was inactivated in lactate dehydrogenase, pyruvate formate lyase, alcohol dehydrogenase, acetate kinase, fumarate reductase, alanine racemase, and methylglyoxal synthase; moreover, onto the chromosome thereof an exogenous L-alanine dehydrogenase gene and alanine racemase gene were integrated. In the present application, by integrating the exogenous L-alanine dehydrogenase gene into the chromosome of the engineering bacterium, the intermediate of glycolysis, pyruvic acid, was converted into L-alanine; by further integrating the exogenous alanine racemase gene, partial L-alanine was converted into D-alanine, achieving the direct production of DL-alanine from raw material saccharides, thereby decreasing the production cycle of DL-alanine, and increasing the yield of DL-alanine.

摘要翻译： 本发明公开了一种DL-丙氨酸生产工程菌。 这种DL-丙氨酸生产工程细菌本身在乳酸脱氢酶，丙酮酸甲酯裂解酶，醇脱氢酶，乙酸激酶，富马酸还原酶，丙氨酸消旋酶和甲基乙二醛合酶中失活; 此外，在其染色体上整合外源L-丙氨酸脱氢酶基因和丙氨酸消旋酶基因。 在本申请中，通过将外源L-丙氨酸脱氢酶基因整合到工程细菌的染色体中，将糖酵解中间体丙酮酸转化为L-丙氨酸; 通过进一步整合外源性丙氨酸消旋酶基因，将部分L-丙氨酸转化为D-丙氨酸，实现从原料糖直接生产DL-丙氨酸，从而降低DL-丙氨酸的生产周期，提高DL-丙氨酸的产量， 丙氨酸。

公开(公告)号：US20120309065A1

公开(公告)日：2012-12-06

申请号：US13518379

申请日：2010-12-21

申请人： Thomas Kvist ,  Marie Just Mikkelsen ,  Rasmus Lund Andersen

发明人： Thomas Kvist ,  Marie Just Mikkelsen ,  Rasmus Lund Andersen

IPC分类号： C12N1/20 ,  C12P7/40 ,  C12P7/02 ,  C12P7/26 ,  C12P3/00 ,  C12N15/74 ,  C12P7/06 ,  C12P7/56 ,  C12P7/16 ,  C12P7/18 ,  C12P7/04 ,  C12P7/28 ,  C12P7/52 ,  C12P7/54 ,  C12P7/46 ,  C12N1/21

CPC分类号： C12R1/01 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1217 ,  C12P7/065 ,  C12P7/10 ,  C12Y101/01027 ,  C12Y203/01008 ,  C12Y207/02001 ,  Y02E50/16 ,  Y02E50/17

摘要： Strict anaerobic thermophilic bacterium belonging to the group of Thermoanaerobacter italicus subsp. marato subsp. nov. and mutants and derivatives thereof. The bacterium is particularly suitable for the production of fermentation products such as ethanol, lactic acid, acetic acid and hydrogen from lignocellulosic biomass.

摘要翻译： 属于热厌氧嗜热细菌亚种的组合的严格的厌氧嗜热细菌 马拉托亚 nov。 及其突变体及衍生物。 该细菌特别适用于从木质纤维素生物质生产发酵产物如乙醇，乳酸，乙酸和氢。

公开(公告)号：US20120276587A1

公开(公告)日：2012-11-01

申请号：US13459067

申请日：2012-04-27

申请人： Zachary Q. Beck ,  Michael C. Miller ,  Caroline M. Peres ,  Yuliya A. Primak ,  Jeff P. Pucci ,  Derek H. Wells

发明人： Zachary Q. Beck ,  Michael C. Miller ,  Caroline M. Peres ,  Yuliya A. Primak ,  Jeff P. Pucci ,  Derek H. Wells

IPC分类号： C12P5/02 ,  C12N1/15 ,  C12N1/19 ,  C12P7/42 ,  C12P23/00 ,  C12P7/00 ,  C12P17/18 ,  C12P7/02 ,  C12P5/00 ,  C12N1/21 ,  C12P7/04

CPC分类号： C12P5/007 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/18 ,  C12N9/88 ,  C12N15/70 ,  C12P5/002 ,  C12P5/026 ,  C12P7/02 ,  C12P7/04 ,  C12P7/42 ,  C12Y101/01027 ,  C12Y101/01034 ,  C12Y101/01037 ,  C12Y102/01051 ,  C12Y102/04001 ,  C12Y203/01008 ,  C12Y203/01016 ,  C12Y203/03001 ,  C12Y203/0301 ,  C12Y207/02001 ,  C12Y301/01031 ,  C12Y401/01031 ,  Y02E50/343

摘要： The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).

摘要翻译： 本发明的特征在于通过在以下酶促途径中通过工程化微生物来增加碳通量以产生甲羟戊酸来生产甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯的组合物和方法：（a）柠檬酸合酶 ）磷酸转乙酰酶，（c）乙酸激酶，（d）乳酸脱氢酶，（e）苹果酸酶和（f）丙酮酸脱氢酶，使得其中一种酶活性被调节。 此外，通过mvaE和mvaS基因的异源表达（例如但不限于来自生物体李斯特氏菌DSM的mvaE和mvaS基因）可以进一步增强甲羟戊酸，异戊二烯，类异戊二烯前体分子和/或类异戊二烯的生产 20601，屎肠球菌，，肠球菌EG2，和黑麦草肠球菌）。