公开(公告)号：US20180179556A1

公开(公告)日：2018-06-28

申请号：US15598316

申请日：2017-05-18

申请人： National Tsing Hua University ,  Chang Chun Plastics Co., Ltd. ,  Chang Chun Petrochemical Co., Ltd.

发明人： Roa-Pu Shen ,  Rex C. Wen

IPC分类号： C12P7/16 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12N9/10 ,  C12N15/70 ,  C12N15/52

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1217 ,  C12N15/52 ,  C12N15/70 ,  C12N2800/101 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y101/0103 ,  C12Y101/01322 ,  C12Y102/01004 ,  C12Y103/01086 ,  C12Y103/05004 ,  C12Y203/01009 ,  C12Y207/02001

摘要： A butanol expression cassette includes a butanol production related genes and a fermentation regulatory element. The fermentation regulatory element controls the expression of the butanol production related gene and locates upstream of the butanol production related gene. The fermentation regulatory element includes a promoter, a ribosome binding site and a transcription factor binding site of a fermentation gene. A fermentation in which the fermentation regulatory element involves includes an acetic acid fermentation, an alcohol fermentation, a succinic acid fermentation or a lactic acid fermentation, the butanol production related gene is not the fermentation gene or a gene of an upstream product of the fermentation in which the fermentation gene involves. The present invention provides a recombinant plasmid formed by cloning the butanol expression cassettes in the expression vector. The present invention also provides a butanol production related gene expression method to express butanol production related gene by using recombinant plasmid.