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    MUTANT YQHD ENZYME FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION
    2.
    发明申请
    MUTANT YQHD ENZYME FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION 有权
    通过发酵生产生物化学品的突变YQHD酶

    公开(公告)号：US20120122169A1

    公开(公告)日：2012-05-17

    申请号：US13387347

    申请日：2010-07-30

    申请人： Francois Voelker ,  Laurence Dumon-Seignovert ,  Philippe Soucaille

    发明人： Francois Voelker ,  Laurence Dumon-Seignovert ,  Philippe Soucaille

    IPC分类号： C12P7/18 ,  C12N1/19 ,  C12N15/53 ,  C12N1/21 ,  C12P7/26 ,  C12N9/02

    CPC分类号： C12P7/26 ,  C12N9/0006 ,  C12N9/88 ,  C12P7/18 ,  C12Y101/01002 ,  C12Y101/01006 ,  C12Y402/03003

    摘要： The present invention concerns a method for the production of a biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol comprising culturing a microorganism modified for an improved production of the biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a YqhD enzyme which catalytic efficiency toward NADPH is increased.The present invention also relates to a mutant YqhD enzyme comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the YqhD activity of the parent enzyme and the catalytic efficiency toward NADPH of the mutant YqhD is increased as compared with the catalytic efficiency toward NADPH of the parent enzyme.

    摘要翻译： 本发明涉及一种选自丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇的生物化学生产方法，该方法包括培养一种改良生物化学生物的微生物， 丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇，并回收可以进一步纯化的所需生化物，其中微生物表达YqhD酶，其对NADPH的催化效率 增加了。 本发明还涉及包含在相同位置的不同氨基酸残基替代的母体酶的蛋白质序列中的至少一个氨基酸残基的突变型YqhD酶，其中突变酶保留了超过50％的YqhD活性 的亲代酶的催化效率和与NADPH的亲和酶的催化效率相比，突变型YqhD的NADPH的催化效率增加。

    ENGINEERING BACTERIA FOR PRODUCING DL-ALANINE AND METHOD FOR PRODUCING DL-ALANINE BY USING ENGINEERING BACTERIA
    4.
    发明申请
    ENGINEERING BACTERIA FOR PRODUCING DL-ALANINE AND METHOD FOR PRODUCING DL-ALANINE BY USING ENGINEERING BACTERIA 审中-公开
    用于生产DL-ALANINE的工程细菌和使用工程细菌生产DL-ALANINE的方法

    公开(公告)号：US20150247174A1

    公开(公告)日：2015-09-03

    申请号：US14395187

    申请日：2012-12-28

    申请人： ANHUI HUAHENG BIOENGINEERING CO., LTD.

    发明人： Xueli Zhang ,  Dongzhu Zhang

    IPC分类号： C12P13/06 ,  C12N15/70 ,  C12N9/90 ,  C12R1/19 ,  C12N9/06

    CPC分类号： C12P13/06 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/90 ,  C12N15/70 ,  C12R1/19 ,  C12Y101/01001 ,  C12Y101/01027 ,  C12Y103/05001 ,  C12Y104/01001 ,  C12Y203/01054 ,  C12Y207/02001 ,  C12Y402/03003 ,  C12Y501/01001

    摘要： The present invention discloses a DL-alanine-producing engineering bacterium. This DL-alanine-producing engineering bacterium itself was inactivated in lactate dehydrogenase, pyruvate formate lyase, alcohol dehydrogenase, acetate kinase, fumarate reductase, alanine racemase, and methylglyoxal synthase; moreover, onto the chromosome thereof an exogenous L-alanine dehydrogenase gene and alanine racemase gene were integrated. In the present application, by integrating the exogenous L-alanine dehydrogenase gene into the chromosome of the engineering bacterium, the intermediate of glycolysis, pyruvic acid, was converted into L-alanine; by further integrating the exogenous alanine racemase gene, partial L-alanine was converted into D-alanine, achieving the direct production of DL-alanine from raw material saccharides, thereby decreasing the production cycle of DL-alanine, and increasing the yield of DL-alanine.

    摘要翻译： 本发明公开了一种DL-丙氨酸生产工程菌。 这种DL-丙氨酸生产工程细菌本身在乳酸脱氢酶，丙酮酸甲酯裂解酶，醇脱氢酶，乙酸激酶，富马酸还原酶，丙氨酸消旋酶和甲基乙二醛合酶中失活; 此外，在其染色体上整合外源L-丙氨酸脱氢酶基因和丙氨酸消旋酶基因。 在本申请中，通过将外源L-丙氨酸脱氢酶基因整合到工程细菌的染色体中，将糖酵解中间体丙酮酸转化为L-丙氨酸; 通过进一步整合外源性丙氨酸消旋酶基因，将部分L-丙氨酸转化为D-丙氨酸，实现从原料糖直接生产DL-丙氨酸，从而降低DL-丙氨酸的生产周期，提高DL-丙氨酸的产量， 丙氨酸。

    Mutant YqhD enzyme for the production of a biochemical by fermentation
    5.
    发明授权
    Mutant YqhD enzyme for the production of a biochemical by fermentation 有权
    用于通过发酵生产生化的突变型YqhD酶

    公开(公告)号：US08969053B2

    公开(公告)日：2015-03-03

    申请号：US13387347

    申请日：2010-07-30

    申请人： François Voelker ,  Laurence Dumon-Seignovert ,  Philippe Soucaille

    发明人： François Voelker ,  Laurence Dumon-Seignovert ,  Philippe Soucaille

    IPC分类号： C12P7/26 ,  C12P1/00 ,  C12N9/04 ,  C12N9/88 ,  C12P7/18

    CPC分类号： C12P7/26 ,  C12N9/0006 ,  C12N9/88 ,  C12P7/18 ,  C12Y101/01002 ,  C12Y101/01006 ,  C12Y402/03003

    摘要： The present invention concerns a method for the production of a biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol comprising culturing a microorganism modified for an improved production of the biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a YqhD enzyme which catalytic efficiency toward NADPH is increased.The present invention also relates to a mutant YqhD enzyme comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the YqhD activity of the parent enzyme and the catalytic efficiency toward NADPH of the mutant YqhD is increased as compared with the catalytic efficiency toward NADPH of the parent enzyme.

    摘要翻译： 本发明涉及一种选自丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇的生物化学生产方法，该方法包括培养一种改良生物化学生物的微生物， 丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇，并回收可以进一步纯化的所需生化物，其中微生物表达YqhD酶，其对NADPH的催化效率 增加了。 本发明还涉及包含在相同位置的不同氨基酸残基替代的母体酶的蛋白质序列中的至少一个氨基酸残基的突变型YqhD酶，其中突变酶保留了超过50％的YqhD活性 的亲代酶的催化效率和与NADPH的亲和酶的催化效率相比，突变型YqhD的NADPH的催化效率增加。