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    LIPOSOME AND METHOD FOR PRODUCING LIPOSOME
    32.
    发明申请
    LIPOSOME AND METHOD FOR PRODUCING LIPOSOME 有权
    用于生产脂质体的脂质体和方法

    公开(公告)号：US20150125393A1

    公开(公告)日：2015-05-07

    申请号：US14598603

    申请日：2015-01-16

    Applicant: Konica Minolta, Inc.

    Inventor： Takeshi ISODA

    IPC: A61K9/127 ,  A61K31/7088 ,  A61K49/00

    CPC classification number: A61K9/1277 ,  A23P10/30 ,  A61K8/14 ,  A61K8/416 ,  A61K8/4986 ,  A61K8/63 ,  A61K8/68 ,  A61K9/1271 ,  A61K9/1272 ,  A61K31/7088 ,  A61K31/7105 ,  A61K31/713 ,  A61K47/62 ,  A61K47/6911 ,  A61K49/0002 ,  A61K49/0466 ,  A61Q11/00 ,  A61Q19/00 ,  B01J13/02 ,  C12N15/88 ,  C12N2810/85

    Abstract: It is intended to provide a liposome preparation which is a liposome, has a lipid bilayer membrane composed of an inner membrane constituted by a lipid including one or more types of functional lipids (a lipid capable of chemically interacting with another compound such as a charged lipid, a polarizable lipid, a lipid-soluble lipid or a water-soluble lipid) and an outer membrane constituted by a lipid with or without including one or more types of functional lipids, and is characterized in that at least a condition that the amount of any one type of functional lipid contained in the inner membrane is larger than in the outer membrane is satisfied. The liposome preparation is suitable as a liposome for encapsulating a contrast agent (a neutral substance having a hydroxy group), siRNA (an anionic substance) having an anticancer activity or the like. Its encapsulation ratio of drug agents, dispersion stability, control release and the like have been improved.

    Abstract translation: 本发明提供一种脂质体制剂，其是具有由包含一种或多种类型的功能性脂质的脂质构成的内膜构成的脂质双层膜（能够与另一种化合物如带电脂质化学相互作用的脂质 ，可极化脂质，脂溶性脂质或水溶性脂质）和由具有或不包含一种或多种类型的功能性脂质的脂质构成的外膜，其特征在于，至少一种条件是， 内膜中含有的任何一种功能性脂质都比外膜要大。 脂质体制剂适合用作包封具有抗癌活性的对比剂（具有羟基的中性物质），siRNA（阴离子物质）的脂质体。 其药剂的包封率，分散稳定性，控制释放等已得到改善。

    Viral Vectors with Improved Properties
    34.
    发明申请
    Viral Vectors with Improved Properties 审中-公开
    具有改进性质的病毒载体

    公开(公告)号：US20120220492A1

    公开(公告)日：2012-08-30

    申请号：US13247860

    申请日：2011-09-28

    Applicant: Thomas Weber ,  Laure Gigout

    Inventor： Thomas Weber ,  Laure Gigout

    IPC: C40B40/08 ,  C40B50/00 ,  C12N15/864 ,  C12Q1/70 ,  C12N5/071 ,  C12N15/86 ,  C40B40/02 ,  C40B50/06

    CPC classification number: C12N15/86 ,  A61K48/0091 ,  C12N7/00 ,  C12N2750/14123 ,  C12N2750/14143 ,  C12N2750/14145 ,  C12N2810/40 ,  C12N2810/405 ,  C12N2810/60 ,  C12N2810/6072 ,  C12N2810/80 ,  C12N2810/85 ,  C12N2810/851 ,  C12N2810/854 ,  C12N2810/859 ,  C12N2830/42 ,  C12N2840/44

    Abstract: Methods to improve the tropism or other features of a virus are disclosed. Such methods can be used to prepare, e.g., DNA or plasmid libraries of variants of a gene encoding a viral capsid or envelope protein having a randomly inserted restriction site, libraries of viral clones with such variant genes with a randomly inserted restriction site or polypeptide sequence targeting a receptor expressed by a specific type of mammalian cells. Described are also methods to prepare mosaic viruses, i.e., viral particles wherein copies of one or more capsid or envelope proteins originate from different sources. These methods can be used to prepare mosaic viruses of a specific mixture of wild-type and mutant proteins, or of different types of mutant proteins.

    Abstract translation: 公开了改善病毒向性或其他特征的方法。 这样的方法可用于制备例如编码具有随机插入的限制性位点的病毒衣壳或包膜蛋白的基因的变体的DNA或质粒文库，具有随机插入的限制性位点或多肽序列的具有这种变体基因的病毒克隆的文库 靶向由特定类型的哺乳动物细胞表达的受体。 还描述了制备花叶病毒的方法，即病毒颗粒，其中一种或多种衣壳或包膜蛋白的拷贝来源于不同的来源。 这些方法可用于制备野生型和突变蛋白或不同类型的突变蛋白的特异性混合物的花叶病毒。

    Nuclear transport nucleic acid delivery vector
    36.
    发明授权
    Nuclear transport nucleic acid delivery vector 失效
    核转运核酸递送载体

    公开(公告)号：US07745596B2

    公开(公告)日：2010-06-29

    申请号：US11632138

    申请日：2005-07-11

    Applicant: Seiji Shinkai ,  Takeshi Nagasaki ,  Takeshi Kawazu ,  Shinji Kakimoto

    Inventor： Seiji Shinkai ,  Takeshi Nagasaki ,  Takeshi Kawazu ,  Shinji Kakimoto

    IPC: C07H21/02 ,  C12N15/00

    CPC classification number: C12N15/88 ,  C07K2319/09 ,  C07K2319/23 ,  C12N15/87 ,  C12N2810/6072 ,  C12N2810/85

    Abstract: To provide a new technique by which efficient transfection is ensured in delivering a target gene into a cell, disclosed is a nucleic acid construct for nuclear import, which comprises a ternary complex consisting of a nucleic acid substance containing a gene to be delivered into the nucleus of a cell, an importin protein (for example, importin-β) capable of passing through the nuclear pore and involved in the nuclear transport, and a binding substance (for example, polyethyleneimine) bound to both of the nucleic acid substance and the importin protein. Nucleic acid transport from outside of a cell into the cell nucleus can be particularly promoted by administering the nucleic acid construct bound to a cell membrane receptor binding factor and/or a membrane fusing substance, or administering the nucleic acid construct encapsulated in a non-viral vector (for example, Sendai virus envelope) having cell membrane permeability and membrane fusing properties.

    Abstract translation: 为了提供一种新的技术，通过该技术确保将靶基因递送到细胞中的有效转染，公开了一种用于核进入的核酸构建体，其包含由含有待递送至细胞核的基因的核酸物质组成的三元复合物 的细胞，能够通过核孔并参与核转运的importin蛋白（例如，进口蛋白）和结合物质（例如聚乙烯亚胺），其结合核酸物质和 importin蛋白。 通过施用与细胞膜受体结合因子和/或膜融合物质结合的核酸构建体，或者将包封在非病毒体内的核酸构建体施用，可以特别地促进从细胞外进入细胞核的核酸 具有细胞膜通透性和膜融合特性的载体（例如仙台病毒包膜）。

    Regulation of angiogenesis with zinc finger proteins
    38.
    发明授权
    Regulation of angiogenesis with zinc finger proteins 失效
    用锌指蛋白调节血管生成

    公开(公告)号：US07560440B2

    公开(公告)日：2009-07-14

    申请号：US11486994

    申请日：2006-07-14

    Applicant: Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    Inventor： Edward Rebar ,  Andrew Jamieson ,  Qiang Liu ,  Pei-Qi Liu ,  Alan Wolffe ,  Stephen P. Eisenberg ,  Eric Jarvis

    IPC: A61K48/00

    CPC classification number: A61K48/005 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/52 ,  C07K2319/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2799/021 ,  C12N2810/85 ,  C12N2830/008

    Abstract: Provided herein are a variety of methods and compositions for regulating angiogenesis, such methods and compositions being useful in a variety of applications where modulation of vascular formation is useful, including, but not limited to, treatments for ischemia and wound healing. Certain of the methods and compositions accomplish this by using various zinc finger proteins that bind to particular target sites in one or more VEGF genes. Nucleic acids encoding the zinc finger proteins are also disclosed. Methods for modulating the expression of one or more VEGF genes with the zinc finger proteins and nucleic acids are also disclosed. Such methods can also be utilized in a variety of therapeutic applications that involve the regulation of endothelial cell growth. Pharmaceutical compositions including the zinc finger proteins or nucleic acids encoding them are also provided.

    Abstract translation: 本文提供了用于调节血管生成的各种方法和组合物，所述方法和组合物可用于血管形成调节是有用的多种应用，包括但不限于缺血和伤口愈合的治疗。 某些方法和组合物通过使用结合一个或多个VEGF基因中的特定靶位点的各种锌指蛋白来实现。 还公开了编码锌指蛋白的核酸。 还公开了用锌指蛋白和核酸调节一种或多种VEGF基因的表达的方法。 这些方法也可用于涉及内皮细胞生长调节的各种治疗应用中。 还提供了包括锌指蛋白或编码它们的核酸的药物组合物。

    Method And Kit For Expressing Protein Under Regulation Of The Expression From Repeated Sequence Formed By Gene Amplification, And Transformant
    39.
    发明申请
    Method And Kit For Expressing Protein Under Regulation Of The Expression From Repeated Sequence Formed By Gene Amplification, And Transformant 审中-公开
    用于表达蛋白质的方法和试剂盒用于通过基因扩增和转化体形成的重复序列表达调控

    公开(公告)号：US20070298458A1

    公开(公告)日：2007-12-27

    申请号：US11667963

    申请日：2005-11-15

    Applicant: Noriaki Shimizu

    Inventor： Noriaki Shimizu

    IPC: C12P21/00 ,  C12N5/10

    CPC classification number: C12N15/67 ,  C12N15/85 ,  C12N2810/85 ,  C12N2820/85 ,  C12P21/02

    Abstract: A method is disclosed for releasing the transcriptional regulation caused by a repeated sequence in a gene, a kit therefor and so on to thereby establish a system capable of producing a protein in a large amount. At least one embodiment of the method can be achieved by any one or more of the following methods: (a) in the amplification of a gene encoding a target protein, co-amplifying a polynucleotide of 10 kbp or more such as a λ-phage DNA or an insulator sequence; (b) selecting by culturing cells having undergone gene amplification in media containing a drug with a gradual increase in concentration; (c) elevating the promoter activity of inducing the expression of a gene encoding a target protein; (d) excising an amplified gene region from a chromosome with the use of Cre-LoxP System; (e) treating cells having undergone gene amplification with 5-aza-2′-deoxycytidine to thereby lower the methylation degree of DNA; and (f) selecting the mammalian cells having undergone gene amplification on double minute chromosomes.

    Abstract translation: 公开了一种用于释放由基因中的重复序列引起的转录调控的方法，其用于其的试剂盒等，从而建立能够大量产生蛋白质的系统。 该方法的至少一个实施方案可以通过以下任何一种或多种方法实现：（a）扩增编码靶蛋白的基因，共扩增10kbp或更多的多核苷酸，例如λ噬菌体 DNA或绝缘子序列; （b）通过培养在具有逐渐增加浓度的药物的培养基中培养经历基因扩增的细胞进行选择; （c）提高诱导编码靶蛋白的基因表达的启动子活性; （d）使用Cre-LoxP系统从染色体切除扩增的基因区域; （e）处理已经用5-氮杂-2'-脱氧胞苷进行基因扩增的细胞，从而降低DNA的甲基化程度; 和（f）选择在双分子染色体上经历基因扩增的哺乳动物细胞。

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