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    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS
    31.
    发明申请
    INTEGRATED SENSOR ARRAYS FOR BIOLOGICAL AND CHEMICAL ANALYSIS 审中-公开
    用于生物和化学分析的集成传感器阵列

    公开(公告)号：US20150160154A1

    公开(公告)日：2015-06-11

    申请号：US14569372

    申请日：2015-01-21

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. ROTHBERG ,  James Bustillo ,  Mark James Milgrew ,  Jonathan Schultz ,  David Marran ,  Todd Rearick ,  Kim L. Johnson

    IPC: G01N27/414 ,  C12Q1/68

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

    Abstract: The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.

    Abstract translation: 本发明涉及包括大规模化学场效应晶体管阵列的装置和芯片，其包括能够保留来自样品流体的化学或生物样品用于分析的样品保持区阵列。 在一个方面，这种晶体管阵列具有10μm或更小的间距，并且每个采样保持区域位于至少一个化学场效应晶体管上，该晶体管被配置为产生与化学或生物样品的特性相关的至少一个输出信号 在这样的样品保留区域中。 在一个实施方案中，所述化学或生物样品的特征是带电物质的浓度，并且其中每个所述化学场效应晶体管是离子敏感场效应晶体管，其具有在其表面上具有介电层的浮置栅极， 电介质层与所述样品流体接触并且能够与所述样品流体中的带电物质的浓度成比例地积累电荷。 在一个实施方案中，这种带电物质是氢离子，使得传感器测量样品流体在其样品保持区域中或其附近的pH值的变化。 本发明的装置和芯片可适用于大规模的基于pH的DNA测序和其他生物科学和生物医学应用。

    AMPLIFICATION AND ARRAY LOADING APPARATUS AND METHODS
    32.
    发明申请
    AMPLIFICATION AND ARRAY LOADING APPARATUS AND METHODS 有权
    放大和阵列装载装置和方法

    公开(公告)号：US20130288873A1

    公开(公告)日：2013-10-31

    申请号：US13828633

    申请日：2013-03-14

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kristopher BARBEE ,  Ryan Jones ,  Sean McCusker ,  Maximilan Carpino ,  John Leamon ,  Jonathan Schultz ,  Jon A. Hoshizaki

    IPC: B04B13/00

    CPC classification number: C12Q1/6806 ,  B04B5/0421 ,  B04B13/00 ,  C12Q1/6869 ,  G01N35/00693 ,  G01N35/1011 ,  G01N2035/00495 ,  G01N2035/1013 ,  G01N2035/103

    Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm.

    Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集颗粒的装置和用于加载颗粒的装置 颗粒到传感器阵列上。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。

    Windowed sequencing
    34.
    发明授权
    Windowed sequencing 有权

    公开(公告)号：US11028438B2

    公开(公告)日：2021-06-08

    申请号：US16866653

    申请日：2020-05-05

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd Rearick ,  Mark Milgrew ,  Jonathan Schultz ,  Chris Papalias ,  Kim Johnson

    IPC: C12Q1/6874 ,  G16B30/00

    Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors.

    Windowed sequencing
    36.
    发明授权
    Windowed sequencing 有权

    公开(公告)号：US10655175B2

    公开(公告)日：2020-05-19

    申请号：US16153898

    申请日：2018-10-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Todd Rearick ,  Mark Milgrew ,  Jonathan Schultz ,  Chris Papalias ,  Kim Johnson

    IPC: C12Q1/6874 ,  G16B30/00

    Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.

    System and method incorporating solid buffer
    38.
    发明授权
    System and method incorporating solid buffer 有权

    公开(公告)号：US10107825B2

    公开(公告)日：2018-10-23

    申请号：US14741084

    申请日：2015-06-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： James A. Ball ,  Jonathan Schultz

    IPC: C12Q1/68 ,  G01N33/84 ,  C12Q1/6869 ,  B01J19/00

    Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

    Pinch flow regulator
    39.
    发明授权
    Pinch flow regulator 有权

    公开(公告)号：US10107403B2

    公开(公告)日：2018-10-23

    申请号：US15407333

    申请日：2017-01-17

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz

    IPC: B01L3/00 ,  F16K7/07 ,  G05D16/06

    Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.

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