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    Sequencing device
    2.
    发明授权
    Sequencing device 有权

    公开(公告)号：US10894982B2

    公开(公告)日：2021-01-19

    申请号：US16289508

    申请日：2019-02-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan Schultz ,  Todd Roswech ,  Jon A. Hoshizaki ,  Albert L. Carrillo ,  James A. Ball

    IPC: C12Q1/6869 ,  C12Q1/6806 ,  B01L3/00 ,  G01N27/414 ,  G01N35/10 ,  B01F5/06 ,  B01F11/00 ,  B01F1/00

    Abstract: A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. Each reagent enclosure includes a reagent container including a reagent and an internal cavity defining a compressible volume, an opening defined through the reagent container to the internal cavity. The method further includes connecting a plurality of fluid ports to the openings of the plurality of reagent enclosures; applying a solution through the fluid ports to at least partially fill the plurality of reagent enclosures; and cycling a pressure of the cavity, whereby for each of the reagent enclosures, during increasing pressure, the solution enters the internal cavity of the reagent container, combines with the reagent, and compresses the compressible volume, and during decreasing pressure, the compressible volume decreases and the reagent is ejected through the opening.

    System and method for emulsion breaking
    4.
    发明授权
    System and method for emulsion breaking 有权
    破乳系统和方法

    公开(公告)号：US09533240B2

    公开(公告)日：2017-01-03

    申请号：US14536429

    申请日：2014-11-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brian Reed ,  James A. Ball

    IPC: C07H21/00 ,  C07H1/06 ,  C12Q1/68 ,  B01D17/04

    CPC classification number: B01D17/047 ,  B01D17/04 ,  B01D17/041 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2563/159

    Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

    Abstract translation: 从乳液中回收珠粒载体的方法包括将表面活性剂水溶液供给到离心管中; 在离心管中的表面活性剂溶液上提供疏水性液体，其中表面活性剂水溶液的体积与疏水性液体的体积之比不大于0.5; 并且在离心时将乳液施加在疏水性液体上，所述乳液包含分散的水相，包括珠粒支持体，乳液破碎和分散相的材料优先分配至表面活性剂溶液。

    SYSTEM AND METHOD FOR EMULSION BREAKING
    9.
    发明申请
    SYSTEM AND METHOD FOR EMULSION BREAKING 有权

    公开(公告)号：US20220193576A1

    公开(公告)日：2022-06-23

    申请号：US17566511

    申请日：2021-12-30

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Brian Reed ,  James A. Ball

    IPC: B01D17/04 ,  C07H1/06 ,  C07H21/00 ,  C12Q1/6806

    Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.

    System and method incorporating solid buffer
    10.
    发明授权
    System and method incorporating solid buffer 有权

    公开(公告)号：US11131679B2

    公开(公告)日：2021-09-28

    申请号：US16149799

    申请日：2018-10-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： James A. Ball ,  Jonathan Schultz

    IPC: G01N33/84 ,  B01J19/00 ,  C12Q1/68 ,  C12Q1/6869

    Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

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