公开(公告)号：US10077290B2

公开(公告)日：2018-09-18

申请号：US14367973

申请日：2012-12-31

申请人： Dana-Farber Cancer Institute, Inc.

发明人： Loren D. Walensky ,  Gregory H. Bird

IPC分类号： A61K39/155 ,  C07K14/005 ,  A61K47/48 ,  C12N7/00 ,  G01N33/68 ,  A61K39/12 ,  A61K38/00

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/12 ,  A61K39/155 ,  A61K47/60 ,  C12N7/00 ,  C12N2760/18522 ,  C12N2760/18533 ,  C12N2760/18534 ,  C12N2760/18571 ,  G01N33/68 ,  G01N2333/135 ,  G01N2500/02

摘要： Internally cross-linked peptides useful for interfering with Respiratory Syncytial Virus (RSV) infection is based on RSV-F protein. These peptides are capable of reducing infection in cellular and animal models.

公开(公告)号：US10017562B2

公开(公告)日：2018-07-10

申请号：US15638820

申请日：2017-06-30

申请人： AIMM THERAPEUTICS B.V.

发明人： Tim Beaumont ,  Etsuko Yasuda

IPC分类号： C07K16/10 ,  G01N33/569 ,  A61K39/00

CPC分类号： C07K16/1027 ,  A61K2039/505 ,  A61K2039/507 ,  C07K2317/21 ,  C07K2317/30 ,  C07K2317/34 ,  C07K2317/76 ,  G01N33/56983 ,  G01N2333/135

摘要： The invention relates to human isolated, synthetic or recombinant antibodies or functional parts thereof, specific for the RSV G protein. Antibodies specific for the RSV G protein are particularly suitable for counteracting RSV and symptoms, such as inflammation, resulting from an RSV infection. The invention further relates to the use of such RSV G-specific antibodies for diagnosis of an RSV infection and as a medicament and/or prophylactic agent for at least in part treating or alleviating symptoms of a Respiratory Syncytial Virus infection and/or a Respiratory Syncytial Virus related disorder.

公开(公告)号：US09403900B2

公开(公告)日：2016-08-02

申请号：US14297486

申请日：2014-06-05

申请人： Crucell Holland B.V.

发明人： Robert Anthony Williamson ,  Jehangir Wadia ,  Gabriel Pascual ,  Elissa Keogh

IPC分类号： C07K16/10 ,  G01N33/569 ,  A61K39/42 ,  C07K16/46 ,  C12N7/00 ,  A61K31/7056 ,  A61K39/00

CPC分类号： C07K16/1027 ,  A61K31/7056 ,  A61K39/42 ,  A61K45/06 ,  A61K2039/505 ,  A61K2039/507 ,  A61K2300/00 ,  C07K16/10 ,  C07K16/468 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/35 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  C12N7/00 ,  C12N2760/18534 ,  C12N2760/18571 ,  G01N33/56983 ,  G01N2333/135

摘要： Provided herein are antibodies or antigen-binding fragments thereof that immunospecifically bind to the fusion (F) protein of Respiratory Syncytial Virus (RSV). Also provided are methods for of prevention, treatment and diagnosis of viral infection and/or the treatment of one more symptoms of RSV-mediated disease. Methods of generating antibodies that immunospecifically bind RSV F protein also are provided.

公开(公告)号：US09354230B2

公开(公告)日：2016-05-31

申请号：US12977271

申请日：2010-12-23

申请人： Eli N. Glezer ,  Kent Johnson ,  Michael Tsionsky ,  John H. Kenten ,  Jeff D. Debad ,  Robert M. Umek ,  Paula Denney Eason ,  Hans Biebuyck ,  Jacob N. Wohlstadter ,  James Wilbur ,  George Sigal ,  Niranjan Y. Sardesai

发明人： Eli N. Glezer ,  Kent Johnson ,  Michael Tsionsky ,  John H. Kenten ,  Jeff D. Debad ,  Robert M. Umek ,  Paula Denney Eason ,  Hans Biebuyck ,  Jacob N. Wohlstadter ,  James Wilbur ,  George Sigal ,  Niranjan Y. Sardesai

IPC分类号： G01N33/543 ,  C12Q1/48 ,  G01N33/68

CPC分类号： G01N33/5304 ,  C12Q1/485 ,  G01N33/54366 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/54386 ,  G01N33/56944 ,  G01N33/56983 ,  G01N33/56988 ,  G01N33/573 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6842 ,  G01N33/6845 ,  G01N2333/11 ,  G01N2333/135 ,  G01N2333/16 ,  G01N2333/525 ,  G01N2333/5412 ,  G01N2333/545 ,  G01N2333/57 ,  G01N2333/71 ,  G01N2333/9121 ,  Y10S436/807

摘要： Multiplexed test measurements are conducted using an assay module having a plurality of assay domains. In preferred embodiments, these measurements are conducted in assay modules having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

公开(公告)号：US20140363427A1

公开(公告)日：2014-12-11

申请号：US14297486

申请日：2014-06-05

申请人： Crucell Holland B.V.

发明人： Robert Anthony Williamson ,  Jehangir Wadia ,  Gabriel Pascual ,  Elissa Keogh

IPC分类号： C07K16/10 ,  G01N33/569 ,  A61K31/7056 ,  C12N7/00 ,  C07K16/46 ,  A61K39/42

CPC分类号： C07K16/1027 ,  A61K31/7056 ,  A61K39/42 ,  A61K45/06 ,  A61K2039/505 ,  A61K2039/507 ,  A61K2300/00 ,  C07K16/10 ,  C07K16/468 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/33 ,  C07K2317/35 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  C12N7/00 ,  C12N2760/18534 ,  C12N2760/18571 ,  G01N33/56983 ,  G01N2333/135

摘要： Provided herein are antibodies or antigen-binding fragments thereof that immunospecifically bind to the fusion (F) protein of Respiratory Syncytial Virus (RSV). Also provided are methods for of prevention, treatment and diagnosis of viral infection and/or the treatment of one more symptoms of RSV-mediated disease. Methods of generating antibodies that immunospecifically bind RSV F protein also are provided.

摘要翻译： 本文提供了免疫特异性结合呼吸道合胞病毒（RSV）的融合（F）蛋白的抗体或其抗原结合片段。 还提供了用于预防，治疗和诊断病毒感染和/或治疗另一种RSV介导的疾病症状的方法。 还提供产生免疫特异性结合RSV F蛋白的抗体的方法。

公开(公告)号：US20140348858A1

公开(公告)日：2014-11-27

申请号：US14360219

申请日：2012-11-23

申请人： PONTIFICIA UNIVERSIDAD CATOLICA DE CHILE

发明人： Alexis Mikes Kalergis Parra ,  Susan Marcela Bueno Ramirez ,  Jorge Eugenio Mora Alarcon ,  Roberto Sebastian Gomez Johnson

IPC分类号： C07K16/10 ,  G01N33/569

CPC分类号： C07K16/1027 ,  C07K2317/20 ,  C07K2317/56 ,  G01N33/56983 ,  G01N2333/135

摘要： The use of monoclonal antibodies specific for respiratory syncytial virus (RSV). Specifically, to a monoclonal antibody IgG2A secreted by the cell line of 8A4/G9 hybridoma specifically directed to the M2-1 viral antigen, which is associated with the nucleocapside of the virus. The antibodies can be used for assays for the detection and/or determination of RSV infection. The antibodies are in the pure state and do not contain any other contaminating biological material. A method for preventing and treating the infection caused by respiratory syncytial virus (RSV) in a given host is provided, including the administration of a composition containing the monoclonal antibodies secreted by the 8A4/G9 hybridoma in sufficient doses to prevent the disease. The antibody can be humanized in order to minimize the possibility of an immune response against the same in the patient. In addition, it can be used to obtain any pharmaceutical form of the formulation of the monoclonal antibodies secreted by the 8A4/G9 hybridoma, which are suitable for the treatment or prevention of the disease caused by RSV. It also provides methods for detection and diagnosis of RSV viral antigens in biological samples using the monoclonal antibodies produced and secreted by cells of the 8A4/G9 hybridoma.

摘要翻译： 使用特异于呼吸道合胞病毒（RSV）的单克隆抗体。 具体地说，涉及由特异性针对M2-1病毒抗原的与病毒核衣壳相关的8A4 / G9杂交瘤细胞系分泌的单克隆抗体IgG2A。 抗体可用于检测和/或测定RSV感染。 抗体处于纯状态，不含任何其他污染的生物材料。 提供了用于预防和治疗给定宿主中由呼吸道合胞病毒（RSV）引起的感染的方法，包括以足够的剂量施用含有由8A4 / G9杂交瘤分泌的单克隆抗体的组合物以预防疾病。 抗体可以被人源化以便最小化对患者的免疫应答的可能性。 另外，可以用于获得由8A4 / G9杂交瘤分泌的单克隆抗体制剂的任何药物形式，其适用于治疗或预防由RSV引起的疾病。 它还提供使用由8A4 / G9杂交瘤细胞产生和分泌的单克隆抗体来检测和诊断生物样品中RSV病毒抗原的方法。

公开(公告)号：US20130244223A1

公开(公告)日：2013-09-19

申请号：US13755599

申请日：2013-01-31

申请人： Janssen Infectious Diseases-Diagnostics

发明人： David Nauwelaers ,  Lieven Jozef Stuyver

IPC分类号： C12Q1/70

CPC分类号： C12Q1/70 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q1/701 ,  G01N2333/135 ,  C12Q2545/114 ,  C12Q2531/113

摘要： The invention concerns a method for the extraction of nucleic acids from biological samples e.g. tissue material or sputum derived from human or animal species and the quantitative detection thereafter of said nucleic acids e.g. in terms of viral load, more specifically RSV viral load detection.

摘要翻译： 本发明涉及从生物样品中提取核酸的方法，例如 来自人或动物物种的组织材料或痰，以及其后所述核酸的定量检测。 在病毒载量方面，更具体地说是RSV病毒载量检测。

公开(公告)号：US08137921B2

公开(公告)日：2012-03-20

申请号：US12513594

申请日：2007-12-12

申请人： Dirk André E Roymans ,  Koenraad Lodewijk August van Acker ,  Inge Vereycken ,  Géry Karel Julia Dams

发明人： Dirk André E Roymans ,  Koenraad Lodewijk August van Acker ,  Inge Vereycken ,  Géry Karel Julia Dams

IPC分类号： G01N33/53 ,  A61K39/155

CPC分类号： G01N21/6428 ,  C07K14/005 ,  C12N2760/18522 ,  G01N21/6408 ,  G01N33/542 ,  G01N2021/6441 ,  G01N2333/135

摘要： The present invention concerns a fluorescence resonance energy transfer based high throughput test system to measure the formation of the RSV F1 six-helix bundle. In a first embodiment the current invention relates to a homogeneous time resolved fluorescence-based test system comprising a first helical polypeptide consisting essentially of the sequence of IQN57 (SEQ ID NO: 1); a second helical polypeptide consisting essentially of the sequence of C45 (SEQ ID NO: 2) wherein said IQN57 is labeled with a light emitting fluorophore and said C45 is labeled with an ultra-violet excitable fluorophore.

摘要翻译： 本发明涉及一种基于荧光共振能量转移的高通量测试系统来测量RSV F1六螺旋束的形成。 在第一实施方案中，本发明涉及基于均匀时间分辨荧光的测试系统，其包含基本上由IQN57（SEQ ID NO：1）序列组成的第一螺旋多肽; 基本上由C45（SEQ ID NO：2）的序列组成的第二螺旋多肽，其中所述IQN57用发光荧光团标记，并且所述C45用紫外线可激发的荧光团标记。

公开(公告)号：US07815914B2

公开(公告)日：2010-10-19

申请号：US11394609

申请日：2006-03-31

申请人： Matthew C. Coffey

发明人： Matthew C. Coffey

IPC分类号： A61K39/38 ,  A61K39/12 ,  C12Q1/24

CPC分类号： G01N33/6872 ,  A61K35/765 ,  A61K45/06 ,  C12N2720/12232 ,  C12Q1/485 ,  G01N33/57407 ,  G01N33/57415 ,  G01N33/57496 ,  G01N2333/135 ,  G01N2333/14 ,  A61K2300/00

摘要： The present invention relates to methods of identifying the susceptibility of cells to reovirus infection by measuring constitutive ras-MAP signaling. The invention also pertains to methods using reovirus for the treatment of cellular proliferative disorders, and particularly cellular proliferative disorders wherein the proliferating cells exhibit constitutive MAPK phosphorylation, in mammals. In particular, the methods provide for reovirus treatment of mammals to treat proliferative disorders which include breast tumors, a subset of tumors in which mutation of the ras gene is not believed to play a significant role.

公开(公告)号：US20100261155A1

公开(公告)日：2010-10-14

申请号：US12663418

申请日：2008-06-06

申请人： Mark E. Peeples ,  Matthew Kennedy ,  William Ray

发明人： Mark E. Peeples ,  Matthew Kennedy ,  William Ray

IPC分类号： C12Q1/70 ,  C07K14/005

CPC分类号： C07K14/005 ,  C12N2760/18522 ,  C12Q1/18 ,  G01N2333/135

摘要： Provided herein are isolated paramyxovirus pre-triggered fusion proteins, or functional fragments thereof, which contain one or more CRAC domains in a location that is away from the transmembrane domain. Also provided herein is a computer model of the structure of the pre-triggered F protein. Compositions that directly or indirectly bind and interfere with the normal activity or binding of the pre-triggered F proteins, or the CRAC domains, are useful as antiviral agents in the treatment of paramyxovirus infections. Thus, disclosed herein are methods of screening for antiviral agents, using the isolated pre-triggered F protein, or functional fragments thereof.

摘要翻译： 本文提供了分离的副粘病毒预触发融合蛋白或其功能片段，其在远离跨膜结构域的位置含有一个或多个CRAC结构域。 本文还提供了预触发F蛋白的结构的计算机模型。 直接或间接结合并干扰预触发的F蛋白或CRAC结构域的正常活性或结合的组合物可用作治疗副粘病毒感染的抗病毒剂。 因此，本文公开了使用分离的预触发F蛋白或其功能片段筛选抗病毒剂的方法。