公开(公告)号：US06399305B1

公开(公告)日：2002-06-04

申请号：US09588950

申请日：2000-06-07

申请人： Yoshihiko Makino ,  Yoshihiko Abe ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita ,  Masashi Ogawa

发明人： Yoshihiko Makino ,  Yoshihiko Abe ,  Makoto Takagi ,  Shigeori Takenaka ,  Kenichi Yamashita ,  Masashi Ogawa

IPC分类号： C12Q168

CPC分类号： C12Q1/6825 ,  Y10T436/143333 ,  C12Q2563/173 ,  C12Q2563/113

摘要： A method of analyzing a nucleic acid fragment sample to judge whether the nucleic acid fragment sample is uncomplementary, partly complementary or complementary to a DNA fragment in its specific base sequence is conducted by the steps of: bringing an aqueous solution of the nucleic acid fragment sample into contact with a DNA chip having an electroconductive substrate and the DNA fragment fixed onto the substrate in the presence of an electrochemical thread intercalator; measuring an electric current flowing from or to the electroconductive substrate along the DNA fragment under application of a potential to the substrate; and comparing the electric current measured above with a referential electric current which is prepared employing a DNA chip equivalent to the above DNA chip, the intercalator, and an aqueous solution of a nucleic acid fragment which is complementary to the DNA fragment of the DNA chip.

摘要翻译： 分析核酸片段样品以判断核酸片段样品是否与其特异性碱基序列中的DNA片段不互补，部分互补或互补的方法通过以下步骤进行：使核酸片段样品的水溶液 与具有导电性基体的DNA芯片接触，并且在电化学线性嵌入剂的存在下固定在基材上的DNA片段;测量在施加电位下沿着DNA片段向基片流向或流到导电基底的电流 ; 并使用与上述DNA芯片相当的DNA芯片，嵌入剂和与DNA芯片的DNA片段互补的核酸片段的水溶液制备的参考电流来比较上述电流。

公开(公告)号：US5518687A

公开(公告)日：1996-05-21

申请号：US419536

申请日：1995-04-10

申请人： Yoshihiro Seto ,  Fumio Sugaya ,  Yoshihiko Abe ,  Yoshiyuki Doi

发明人： Yoshihiro Seto ,  Fumio Sugaya ,  Yoshihiko Abe ,  Yoshiyuki Doi

IPC分类号： G01N35/00 ,  G01N37/00

CPC分类号： G01N35/00029 ,  G01N2035/00059 ,  G01N2035/00089 ,  G01N2035/00108 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/114165

摘要： A chemical analysis film cartridge with a box-like casing body having first and second end walls opposed to each other between which a plurality of dry frameless chemical analysis film chips are stored in a stack. A first opening is formed in a side wall of the casing body near the first end wall so that only the film chip nearest the first end wall can be taken out of the casing body through the first opening by moving the film chip in a direction perpendicular to the stack. A second openings which permits a suction pad to hold the film chip nearest the first end wall and to remove it from the casing body through the first openings is formed in the first end wall. The width of the first opening is larger than the thickness of one film chip and smaller than double the thickness of one film chip over at least a portion of the first opening. The suction pad is retracted with the film chip so that the film chip abuts against the edge of the second opening on opposite sides of the suction pads and then the suction pad is further retracted to pull the central portion of the film chip in this state into the second opening, thereby reshaping the film chip into a predetermined warped-shape conforming to the shape of the first opening.

摘要翻译： 一种具有盒状壳体的化学分析胶片暗盒，其具有彼此相对的第一和第二端壁，多个无框架化学分析膜芯片之间堆叠。 第一开口形成在第一端壁附近的壳体的侧壁中，使得只有最靠近第一端壁的膜片可以通过第一开口从壳体主体中沿垂直方向移动 到堆栈 在第一端壁中形成有允许吸盘将最靠近第一端壁固定在膜芯片上并通过第一开口从壳体移除的第二开口。 第一开口的宽度大于一个薄膜芯片的厚度，并且小于第一开口的至少一部分上的一个薄膜芯片的厚度的两倍。 吸盘与薄膜芯片一起缩回，使得薄膜芯片抵靠在吸盘的相对侧上的第二开口的边缘，然后进一步缩回吸垫，以在该状态下将薄膜芯片的中心部分拉入 第二开口，从而将薄膜芯片重塑成符合第一开口形状的预定扭曲形状。

公开(公告)号：US20080058469A1

公开(公告)日：2008-03-06

申请号：US10592956

申请日：2005-03-11

申请人： Yoshihiko Abe ,  Yutaro Sonoda ,  Jun Konishi ,  Takao Anzai ,  Miyuki Shimizu

发明人： Yoshihiko Abe ,  Yutaro Sonoda ,  Jun Konishi ,  Takao Anzai ,  Miyuki Shimizu

IPC分类号： A61L31/00 ,  A61K31/715 ,  A61P41/00 ,  C08B31/02

CPC分类号： A61L31/042 ,  A61K31/715 ,  C08B31/02 ,  C08B37/0012 ,  C08B37/0018 ,  C08B37/0021

摘要： An adhesion preventive material including a cross-linking polysaccharide derivative containing at least one active ester group introduced in a polysaccharide side chain, which is capable of reacting with an active hydrogen-containing group, and being capable of forming a crosslinked material due to covalent binding among the active ester group and an active hydrogen-containing group upon contact with water under an alkaline condition, is disclosed. The adhesion preventive material is able to reduce preparation works to be carried out while previously estimating the time of application and dose not require a special device.

摘要翻译： 一种防粘连材料，包括含有至少一种引入多糖侧链的活性酯基的交联多糖衍生物，其能够与含活性氢基团反应，并且能够由于共价结合而形成交联材料 在碱性条件下与水接触时，活性酯基和活性含氢基团中。 防粘连材料能够减少准备工作，同时预先估计施药时间，不需要特殊装置。

公开(公告)号：US07321831B2

公开(公告)日：2008-01-22

申请号：US10231242

申请日：2002-08-30

申请人： Yoshihiko Makino ,  Yoshihiko Abe

发明人： Yoshihiko Makino ,  Yoshihiko Abe

IPC分类号： G01N33/48 ,  C12Q1/00 ,  C12Q1/68 ,  G01N33/00 ,  G06F19/00

CPC分类号： C07H21/00 ,  B82Y15/00 ,  B82Y30/00

摘要： It is an object of the present invention to provide a nucleic acid fragment-fixed electrode wherein a probe nucleic acid fragment is fixed on the electrode stably and in an amount-controlled manner. The present invention provides a nucleic acid fragment-fixed electrode wherein a nucleic acid fragment is fixed on the surface of a multi-component self-assembled monolayer of two or more different components which is formed on the electrode, by a covalent bond via a bifunctional linking molecule.

摘要翻译： 本发明的目的是提供一种核酸片段固定电极，其中探针核酸片段稳定地并以量控制的方式固定在电极上。 本发明提供了一种核酸片段固定电极，其中核酸片段固定在多组分自组装单层的两个或多个不同组分的表面上，该组分形成在电极上，通过共价键通过双功能 连接分子。

公开(公告)号：US20070249040A1

公开(公告)日：2007-10-25

申请号：US10587934

申请日：2005-02-02

申请人： Toshihiko Miyamoto ,  Yoshihiko Abe ,  Tomoyuki Iwahori ,  Ayako Nakajima ,  Tetsuo Ayako

发明人： Toshihiko Miyamoto ,  Yoshihiko Abe ,  Tomoyuki Iwahori ,  Ayako Nakajima ,  Tetsuo Ayako

IPC分类号： C12M1/34

CPC分类号： C12M23/08 ,  C12M23/34 ,  C12M23/38 ,  C12M33/02 ,  C12M37/00

摘要： A portable bacteria detector not only simply capable of inspecting the presence of food-poisoning bacteria and the like but also provided with a disinfecting or sterilizing means itself has been widely used. As its application fields spread, the detector has been required to be more inexpensive and be constituted in a form allowing easier waste disposal. In a portable bacteria detector provided with a culture medium and a disinfectant or germicide, constituent members for the detector are formed of plastic materials which are easy to incinerate. Furthermore, once a sample has been collected and a process for detecting the presence of bacteria has been started, the detector can be operated in a completely closed system. In this bacteria detector, an external force is applied from a direction perpendicular to the axis of the detector to break off a fragile part inside the detector, thereby leading to the performance of a liquid chamber opening operation.

摘要翻译： 便携式细菌检测器不仅简单地能够检查食物中毒细菌等的存在，而且还具有消毒或杀菌手段本身已被广泛使用。 随着其应用领域的扩展，检测器被要求更便宜，并且以允许更容易的废物处理的形式构成。 在具有培养基和消毒剂或杀菌剂的便携式细菌检测器中，用于检测器的构成部件由易于焚烧的塑料材料形成。 此外，一旦收集样品并且已经开始检测细菌存在的过程，则检测器可以在完全封闭的系统中操作。 在这种细菌检测器中，从垂直于检测器的轴线的方向施加外力，以断开检测器内的脆弱部分，从而导致液体室打开操作的执行。

公开(公告)号：US5534224A

公开(公告)日：1996-07-09

申请号：US273131

申请日：1994-07-14

申请人： Yoshihiko Abe

发明人： Yoshihiko Abe

IPC分类号： G01N35/00

CPC分类号： G01N35/00029 ,  G01N2035/00059 ,  G01N2035/00089 ,  G01N2035/00108 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/114165

摘要： A chemical analysis film cartridge having a box-like casing body with opposing first and second end walls and in which dry frameless chemical analysis film chips are stored in a stack between the end walls. A first opening is formed in a side wall of the casing body near the first end wall so that only the film chip nearest the first end wall can be removed from the casing body through the first opening. A second opening in the first end wall permits a suction pad to hold the film chip nearest to the first end wall and to take the film chip out of the casing body through the first opening. The width of the first opening is larger than the thickness of one film chip and smaller than double the thickness of one film chip. The suction pad is retracted so that the film chip abuts against the edge of the second opening on opposite sides of the suction pad, and then the suction pad is further retracted to pull the central portion of the film chip into the second opening, thereby reshaping the film chip into a predetermined warped-shape conforming to the shape of the first opening.

摘要翻译： 一种化学分析胶片暗盒，具有具有相对的第一和第二端壁的盒状壳体，并且其中无框架化学分析膜芯片堆叠在端壁之间。 第一开口形成在壳体主体的靠近第一端壁的侧壁中，使得最靠近第一端壁的膜片可以通过第一开口从壳体移除。 在第一端壁中的第二开口允许吸盘保持最靠近第一端壁的薄膜芯片，并通过第一开口将薄膜芯片从壳体中取出。 第一开口的宽度大于一个薄膜芯片的厚度，并且小于一个薄膜芯片的厚度的两倍。 抽吸垫缩回，使得薄膜芯片抵靠在吸盘的相对侧上的第二开口的边缘，然后进一步缩回吸垫以将薄膜芯片的中心部分拉入第二开口，从而重塑 该胶片芯片成为符合第一开口形状的预定的弯曲形状。

公开(公告)号：US4119443A

公开(公告)日：1978-10-10

申请号：US781469

申请日：1977-03-25

申请人： Yoshihiko Abe ,  Isao Okabe ,  Atsushi Shirota

发明人： Yoshihiko Abe ,  Isao Okabe ,  Atsushi Shirota

IPC分类号： C22C38/00 ,  C21D6/00 ,  C23C8/00 ,  C23C8/32 ,  C23C8/38 ,  C23F7/00

CPC分类号： C23C8/38 ,  C23C8/00

摘要： Hardened machined parts are manufactured from rolled or forged steel which consists essentially of 0.05 to 0.15 wt % of C, up to 0.50 wt % of Si, up to 1.5 wt % of Mn, 0.30 to 1.0 wt % of Cr, 0.50 to 1.0 wt % of Mo, one or more of elements selected from the group consisting of 0.10 to 0.40 wt % of V, 0.03 to 0.20 wt % of Ti, 0.03 to 0.20 wt % of Nb and 0.0005 to 0.0050 wt % of B, the balance being Fe, in the following steps: cooling the steel in air from 900.degree. C to 1,000.degree. C; machining the cooled steel; and subjecting the machined steel to a nitriding treatment at 500.degree. to 650.degree. C for 2 to 8 hours.

摘要翻译： 硬化加工部件由轧制或锻造钢制成，其基本上由0.05至0.15重量％的C，至多0.50重量％的Si，至多1.5重量％的Mn，0.30至1.0重量％的Cr，0.50至1.0重量％ ％的Mo，选自0.10〜0.40重量％V，0.03〜0.20重量％Ti，0.03〜0.20重量％Nb和0.0005〜0.0050重​​量％B的元素中的一种或多种，​​余量为 Fe，按照以下步骤：将空气中的钢从900℃冷却至1000℃; 加工冷却钢; 对加工后的钢进行500〜650℃的氮化处理2〜8小时。

公开(公告)号：US07612920B2

公开(公告)日：2009-11-03

申请号：US10901164

申请日：2004-07-29

申请人： Takezo Fujishige ,  Kiyoshi Kasatani ,  Yoshihiko Abe

发明人： Takezo Fujishige ,  Kiyoshi Kasatani ,  Yoshihiko Abe

IPC分类号： G06F15/00

CPC分类号： G06F21/32 ,  Y10S707/99931

摘要： A control unit of a multifunction image forming apparatus obtains document information concerning copied documents, scanner read documents, and e-mail documents, and stores the obtained document information to a storing unit of a document storing unit. The control unit counts usage frequency concerning each document stored in the document storing unit, and stores the information as usage frequency information. The control unit has the operator, who carries out document searching, input predetermined information. The control unit matches the input information, and determines whether the operator is an authorized person concerning the searched document. In a case where it is determined that the operator is a legitimate authorized person, the control unit displays the information of the documents stored in the document storing unit, in an order of high usage frequency, to a display unit of an input operation unit, based on the usage frequency information.

摘要翻译： 多功能图像形成装置的控制单元获得关于复制文档，扫描仪读取文档和电子邮件文档的文档信息，并将获得的文档信息存储到文档存储单元的存储单元。 控制单元对存储在文档存储单元中的每个文档的使用频率进行计数，并将该信息存储为使用频度信息。 控制单元具有执行文档搜索的操作者，输入预定信息。 控制单元匹配输入信息，并且确定操作者是否是关于所搜索文档的授权人员。 在确定操作者是合法授权人的情况下，控制单元以高使用频率的顺序将存储在文档存储单元中的文档的信息显示给输入操作单元的显示单元， 基于使用频率信息。

公开(公告)号：US20090029481A1

公开(公告)日：2009-01-29

申请号：US11919869

申请日：2006-06-29

申请人： Masayoshi Kojima ,  Hiroyuki Hirai ,  Shigeki Kageyama ,  Yoshihiko Abe

发明人： Masayoshi Kojima ,  Hiroyuki Hirai ,  Shigeki Kageyama ,  Yoshihiko Abe

IPC分类号： G01N33/48 ,  B01J19/08

CPC分类号： G01N33/5434 ,  G01N33/92 ,  G01N2446/80

摘要： An object to be achieved by the present invention is to provide a method for determining the amount of a component contained in a specific lipoprotein fraction in a biological sample using an automatic analyzer, without the requirement of a step of fractionating a sample by centrifugation. The present invention provides a method for separating a target component in a biological sample, which comprises the steps of: (1) causing a biological sample to come into contact with independently dispersed magnetic nanoparticles having a particle size of 50 nm or less, which have anionic functional groups on their surfaces, so as to form an agglutinate of the magnetic nanoparticles and biomolecules capable of interacting with the magnetic nanoparticles; and (2) collecting the agglutinate by an external magnetic field.

摘要翻译： 本发明要实现的目的是提供一种使用自动分析仪测定生物样品中特定脂蛋白部分中所含成分的量的方法，而不需要通过离心分离样品的步骤。 本发明提供一种用于分离生物样品中的目标成分的方法，其包括以下步骤：（1）使生物样品与粒径为50nm以下的独立分散的磁性纳米粒子接触， 在其表面上具有阴离子官能团，以便形成能够与磁性纳米颗粒相互作用的磁性纳米颗粒和生物分子的凝集物; 和（2）通过外部磁场收集凝集物。

公开(公告)号：US20080107566A1

公开(公告)日：2008-05-08

申请号：US11664283

申请日：2005-09-29

申请人： Yoshihiko Abe

发明人： Yoshihiko Abe

IPC分类号： B01J19/00

CPC分类号： G01N33/525 ,  B01L3/5023 ,  B01L2300/0825 ,  B01L2300/0887 ,  B01L2400/084 ,  G01N31/22

摘要： It is an object of the invention to provide a multilayer analytical element having a porous where the property of the film is maintained and which is free from the liquid-amount dependency even in the case where a porous film is laminated. The present invention provides a dry multilayer analytical element for the analysis of a liquid sample which comprises a water-non-transmitting planar support on one side of which at least one functional layer and a porous liquid-sample-developing layer consisting of at least one non-fibrous porous are integrally layered in the mentioned order, wherein the non-fibrous porous film contains a water-soluble polymer in such a manner that it does not interact with the functional layer.

摘要翻译： 本发明的目的是提供一种具有多孔的多层分析元件，其中膜的性质被保持，并且即使在层压多孔膜的情况下也没有液体依赖性。 本发明提供了一种用于分析液体样品的干燥多层分析元件，该液体样品在其一侧包括不透水的平面支撑体，其中至少一个功能层和多孔液体样品显影层由至少一个 非纤维状多孔体以上述顺序一体地层叠，其中非纤维状多孔膜以不与功能层相互作用的方式含有水溶性聚合物。