公开(公告)号：US20170044557A1

公开(公告)日：2017-02-16

申请号：US15304504

申请日：2015-04-16

Applicant: Boehringer Ingelheim RCV GmbH & Co KG ,  Sandoz AG ,  VTU Technology GmbH ,  Lonza Ltd.

Inventor： Brigitte Gasser ,  Diethard Mattanovich ,  Markus Buchetics

IPC: C12N15/81 ,  C12P21/00

CPC classification number: C12N15/815 ,  C12N15/81 ,  C12P21/005

Abstract: The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.

Abstract translation: 本发明在重组生物技术领域，特别是在蛋白质表达领域。 本发明一般涉及从宿主细胞表达目标蛋白质（POI）的方法。 本发明特别涉及改善宿主细胞表达和/或分泌目标蛋白和使用宿主细胞进行蛋白质表达的能力。 本发明还涉及细胞培养技术，更具体地涉及培养细胞以产生用于医疗目的的所需分子或食品。

公开(公告)号：US20170029827A1

公开(公告)日：2017-02-02

申请号：US15304050

申请日：2015-04-16

Applicant: Boehringer Ingelheim RCV GmbH & Co KG ,  Sandoz AG ,  VTU Technology GmbH ,  Lonza Ltd.

Inventor： Brigitte Gasser ,  Diethard Mattanovich ,  Markus Buchetics

IPC: C12N15/67 ,  C07K16/00 ,  C12P21/00

CPC classification number: C12N15/67 ,  C07K14/39 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/55 ,  C12P21/00

Abstract: The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.

Abstract translation: 本发明在重组生物技术领域，特别是在蛋白质表达领域。 本发明一般涉及从宿主细胞表达目标蛋白质（POI）的方法。 本发明特别涉及改善宿主细胞表达和/或分泌目标蛋白和使用宿主细胞进行蛋白质表达的能力。 本发明还涉及细胞培养技术，更具体地涉及培养细胞以产生用于医疗目的的所需分子或食品。

公开(公告)号：US20160200760A1

公开(公告)日：2016-07-14

申请号：US14913337

申请日：2014-08-25

Applicant: BOEHRINGER INGELHEIM RCV GMBH & CO KG ,  SANDOZ AG

Inventor： Rainer HAHN ,  Alois JUNGBAUER ,  Alexandru TREFILOV

IPC: C07K1/18 ,  B01J39/20 ,  C12N9/02 ,  B01J41/04 ,  B01J41/20 ,  B01J41/14 ,  B01J39/04 ,  B01J39/26

CPC classification number: B01J39/20 ,  B01D15/362 ,  B01D15/363 ,  B01J39/04 ,  B01J39/05 ,  B01J39/07 ,  B01J39/26 ,  B01J41/05 ,  B01J41/07 ,  B01J41/14 ,  B01J41/20 ,  B01J47/04 ,  C07K1/18 ,  C07K14/43595 ,  C07K16/00 ,  C12N9/0089 ,  C12Y115/01001

Abstract: The present invention provides novel methods of cell disruption and release of biomolecules from a cell. The invention comprises the use of positively and/or negatively charged microparticles comprising ground resin. It is particularly useful for purification of biomolecules from cell culture.

Abstract translation: 本发明提供细胞破碎和细胞释放生物分子的新方法。 本发明包括使用包含研磨树脂的正和/或带负电荷的微粒。 它对于从细胞培养物中纯化生物分子特别有用。

公开(公告)号：US20140154747A1

公开(公告)日：2014-06-05

申请号：US14173292

申请日：2014-02-05

Applicant: Boehringer Ingelheim RCV GmbH & Co. KG

Inventor： Jochen URTHALER ,  Christine ASCHER ,  Daniel BUCHELI

IPC: C12N15/10 ,  C12P19/34

CPC classification number: C12P19/34 ,  B03D1/028 ,  B03D1/082 ,  B03D1/1462 ,  B03D1/1468 ,  B03D1/1487 ,  B03D1/18 ,  B03D2203/003 ,  C12N15/1003 ,  C12N15/1006

Abstract: A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO2 bubbles on the precipitate floe. The CO2 is released from a carbonate salt during or after neutralization (acidification). The method of the invention is preferably carried out in an automated continuous mode applying devices for lysis and neutralization and a novel device for completely continuous clarification (separation of flocs and clarified lysate).

Abstract translation: 描述了用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解，中和和澄清的步骤，并可进一步扩展。 为了分离裂解物和沉淀物，公开了改进的浮选方法。 这种方法是基于沉淀物上的二氧化碳气泡的附着。 在中和（酸化）期间或之后，CO 2从碳酸盐中释放出来。 本发明的方法优选以自动连续模式施加用于裂解和中和的装置和用于完全连续澄清（分离絮凝物和澄清裂解物）的新装置进行。