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公开(公告)号：US5312909A
公开(公告)日：1994-05-17
申请号：US673833
申请日：1991-03-22
CPC分类号： C12N9/1051 , A21D8/047 , C12N1/18 , C12N9/2402 , C12P19/12 , C12Y302/01028 , Y10S435/942
摘要： A transformed yeast is disclosed, comprising at least one gene encoding neutral trehalase or trehalose-6-phosphate synthase which gene has been modified such that it differs from a corresponding wild-type gene encoding neutral trehalase or trehalose-6-phosphate synthase, the yeast having as a result a different trehalose content from the untransformed parent yeast. The sugar resistance and drying resistance of the yeast compared to those of the untransformed strain are thereby improved.
摘要翻译： 公开了转化的酵母，其包含至少一种编码中性海藻糖酶或海藻糖-6-磷酸合酶的基因，该基因已被修饰，使得其与编码中性海藻糖酶或海藻糖-6-磷酸合酶的相应野生型基因不同，所述酵母 结果是与未转化的亲本酵母具有不同的海藻糖含量。 因此，与未转化菌株相比，酵母的耐糖耐性和耐干燥性得以改善。
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公开(公告)号：US20240101991A1
公开(公告)日：2024-03-28
申请号：US17936076
申请日：2022-09-28
申请人： NOVOZYMES A/S
发明人： Marc Dominique Morant , Tomoko Matsui , Kirk Matthew Schnorr , Shiro Fukuyama , Noriko Tsutsumi , Zhengfang Kang
CPC分类号： C12N9/2408 , C12N15/81 , C12P7/06 , C12Y302/01001 , C12Y302/01003 , C12Y302/01028
摘要： The present invention relates to polypeptides having trehalase activity, particularly derived from Talaromyces. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides for the production of ethanol.
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公开(公告)号：US20230265463A1
公开(公告)日：2023-08-24
申请号：US18183669
申请日：2023-03-14
发明人： Charles F. Rice , Ryan Skinner , Trisha Barrett , Aaron Argyros
CPC分类号： C12P7/10 , C12N1/18 , C12N9/2402 , C12N15/81 , C12P7/20 , C12Y302/01028 , C12P19/12
摘要： The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).
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公开(公告)号：US20230193232A1
公开(公告)日：2023-06-22
申请号：US18049055
申请日：2022-10-24
发明人： Trisha Barrett , Aaron Argyros
CPC分类号： C12N9/2402 , C12N1/18 , C12N9/48 , C12N9/1051 , C12N9/2405 , C12P7/10 , C12Y204/01001 , C12Y302/01028
摘要： The present disclosure concerns a recombinant yeast host cell exhibiting higher stability and, in some embodiments, higher fermentation performance. The recombinant yeast host cell stability has a limited ability to express an hydrolase during its propagation phase. In return, this limits the cleavage of a yeast cellular component during or after propagation which may be detrimental to the stability and/or fermentation performances. The recombinant yeast host cell expresses a heterologous hydrolase under the control of a heterologous promoter (for limiting the expression of the heterologous hydrolase during propagation and favoring the expression of the heterologous hydrolase during fermentation).
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15.发明申请Alcoholic Xylose Fermentation at High Temperatures by the Thermotolerant Yeast Hansenula polymorpha 有权高温下的酒精木糖发酵由耐热酵母汉氏酵母多形汉逊酵母公开(公告)号：US20130084616A1
公开(公告)日：2013-04-04
申请号：US13679188
申请日：2012-11-16
IPC分类号： C12P7/06
CPC分类号： C12P7/06 , C07K14/39 , C12N9/1205 , C12N9/2402 , C12P7/10 , C12Y207/01017 , C12Y302/01028 , Y02E50/16 , Y02E50/17
摘要： Methods and compositions for the production of ethanol from lignocellulosic starting materials are provided herein. Embodiments provide yeast cells of the genus H. polymorpha with one or more modifications, including, for example, an inactive acid trehalase gene, overexpression of xylulokinase, and/or overexpression of heat-shock protein 104.
摘要翻译： 本文提供了用于从木质纤维原料生产乙醇的方法和组合物。 实施方案提供多形汉逊酵母属的酵母细胞，其具有一种或多种修饰，包括例如无活性酸海藻糖酶基因，木酮糖激酶的过表达和/或热休克蛋白104的过表达。
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16.发明申请Regulating metabolism by modifying the level of trehalose-6-phosphate by inhibiting endogenous trehalase levels 审中-公开通过抑制内源性海藻糖酶水平来调节海藻糖-6-磷酸的水平来调节代谢公开(公告)号：US20030177531A1
公开(公告)日：2003-09-18
申请号：US10195962
申请日：2002-07-15
发明人： Oscar Johannes Maria Goddijn , Jan Pen , Josephus Christianus Maria Smeekens , Maria Theresia Smits
IPC分类号： A01H005/00
CPC分类号： C12Y302/01028 , C12N9/2402 , C12N15/8245 , C12N15/8261 , Y02A40/146
摘要： A method of modification of the development and/or composition of cells, tissues, or organs in vivo in plants by inhibiting the level of an endogenous trehalase is provided. The cells, tissues, or organs have been genetically altered to comprise a DNA sequence encoding a trehalase inhibitor. The DNA sequence is capable of expressing an RNA that is at least partially complementary to an RNA produced by a DNA sequence encoding the endogenous trehalase. Alternatively, the DNA sequence comprises a DNA sequence which is identical to a DNA sequence encoding the endogenous trehalase. The modification is other than to increase production or accumulation of trehalose.
摘要翻译： 提供了通过抑制内源性海藻糖酶的水平来修饰植物体内细胞，组织或器官的发育和/或组成的方法。 细胞，组织或器官已被遗传改变，以包含编码海藻糖酶抑制剂的DNA序列。 DNA序列能够表达与由内源性海藻糖酶编码的DNA序列产生的RNA至少部分互补的RNA。 或者，DNA序列包含与编码内源海藻糖酶的DNA序列相同的DNA序列。 该改性除了增加海藻糖的产生或积累之外。
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17.发明授权
公开(公告)号：US5976856A
公开(公告)日：1999-11-02
申请号：US505448
申请日：1995-07-21
IPC分类号： C12N15/09 , C07H1/00 , C07H3/06 , C07H21/04 , C12N1/21 , C12N9/24 , C12N15/00 , C12N15/56 , C12P19/12 , C12P19/14 , C12R1/01 , C12R1/19 , C12N9/26 , C12N9/10 , C12N9/34
CPC分类号： C12P19/12 , C12N9/2414 , C12P19/14 , C12Y302/01001 , C12Y302/01028 , C12N2310/111 , C12N2310/33
摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 69,000-79,000 daltons and a pI of about 5.4-6.4, and forms non-reducing saccharides having a trehalose structure as an end unit from reducing amylaceous saccharides having a degree of glucose polymerization of at least 3. The enzyme has satisfactorily high thermostability, i.e. it is substantially not inactivated even when incubated in an aqueous solution (pH 7.0) at 85.degree. C. for 60 min, and this facilitates the production of such non-reducing saccharides on an industrial scale and in a satisfactorily-high yield.
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公开(公告)号：US5587290A
公开(公告)日：1996-12-24
申请号：US494714
申请日：1995-06-26
CPC分类号： C12N1/18 , C12N9/2402 , C12Q1/6895 , C12Y302/01028
摘要： The invention provides methods and compositions relating to stress tolerant yeast; in particular, yeast mutants deficient in the expression of functional ATH1 gene product (Ath1p). Such yeast have enhanced tolerance to dehydration and freezing, are able to grow to a higher cell density over a range of fermentable carbon source concentrations, are able to produce and/or tolerate higher levels of ethanol, and trehalose. Nucleic acids comprising ATH1 gene sequences are used in hybridization probes and PCR primers, in expression vectors, etc. The invention provides methods for producing a yeast mutant with improved survival ability under stress conditions which involve identifying mutations disrupting ATH1 expression using Ath1-specific reagents or ATH1 hybridization probes or primers.
摘要翻译： 本发明提供了与耐应激酵母相关的方法和组合物; 特别是缺乏功能性ATH1基因产物（Ath1p）表达的酵母突变体。 这种酵母具有增强的脱水和冷冻耐受性，能够在可发酵的碳源浓度的范围内生长到更高的细胞密度，能够产生和/或耐受更高水平的乙醇和海藻糖。 包含ATH1基因序列的核酸用于杂交探针和PCR引物，表达载体等。本发明提供了在胁迫条件下产生具有改善的存活能力的酵母突变体的方法，其涉及使用Ath1特异性试剂来鉴定突变ATH1表达的突变，或 ATH1杂交探针或引物。
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公开(公告)号：US20240254468A1
公开(公告)日：2024-08-01
申请号：US18587167
申请日：2024-02-26
发明人： Trisha Barrett , Aaron Argyros
CPC分类号： C12N9/2402 , C12N1/18 , C12N9/1051 , C12N9/2405 , C12N9/48 , C12P7/10 , C12Y204/01001 , C12Y302/01028
摘要： The present disclosure concerns a recombinant yeast host cell exhibiting higher stability and, in some embodiments, higher fermentation performance. The recombinant yeast host cell stability has a limited ability to express an hydrolase during its propagation phase. In return, this limits the cleavage of a yeast cellular component during or after propagation which may be detrimental to the stability and/or fermentation performances. The recombinant yeast host cell expresses a heterologous hydrolase under the control of a heterologous promoter (for limiting the expression of the heterologous hydrolase during propagation and favoring the expression of the heterologous hydrolase during fermentation).
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公开(公告)号：US11802266B2
公开(公告)日：2023-10-31
申请号：US17344044
申请日：2021-06-10
CPC分类号： C12N1/185 , C12N9/2408 , C12P7/06 , C12R2001/865 , C12Y302/01003 , C12Y302/01028
摘要： The present invention relates to genetically engineered yeasts having a heterologous trehalase gene and fermentation processes for using such yeasts. The yeasts can express trehalase in a quantity sufficient to convert significant amounts of trehalose to glucose, thereby improving the yield of the product in a fermentation, and/or reducing or eliminating the need to add exogenous trehalase to the fermentation. The yeasts can also include other heterologous genes for expressing enzymes useful for improving yield and/or for reducing or eliminating the need to add exogenous enzymes to the fermentation.
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