公开(公告)号：US20160010069A1

公开(公告)日：2016-01-14

申请号：US14872392

申请日：2015-10-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Greif ,  Christian Rudolph ,  Manfred Schmidt ,  Harald Sobek ,  Johann-Peter Thalhofer

IPC: C12N9/12

CPC classification number: C12N9/1247 ,  C12Y207/07006

Abstract: The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference.

Abstract translation: 本公开提供了T7RNA聚合酶的新变体。 根据本发明的T7变体的实施方案包括T7多肽的氨基酸序列的位置723上的半胱氨酸 - 丝氨酸取代。 根据本发明的T7变体的实施方案具有DNA依赖性RNA聚合酶酶活性，并且通过氧化硫醇基形成分子内同型二聚体的趋势降低。 本文公开的T7变体内的氨基酸取代最小程度地影响T7多肽的RNA聚合酶活性。 此外，所公开的实施方案的突变可以任选地与与野生型参考物相比提供增强的热稳定性的突变组合。

公开(公告)号：US09040253B2

公开(公告)日：2015-05-26

申请号：US13087075

申请日：2011-04-14

Applicant: Sushmita Mimi Roy ,  Sunil Bajad ,  Evan Green ,  Henry Heinsohn

Inventor： Sushmita Mimi Roy ,  Sunil Bajad ,  Evan Green ,  Henry Heinsohn

IPC: C12Q1/37 ,  G01N33/68 ,  C12P21/02

CPC classification number: C12P21/06 ,  C07K14/535 ,  C12N1/06 ,  C12N1/20 ,  C12N9/1247 ,  C12P21/02 ,  C12Y207/07006 ,  G01N33/6848

Abstract: The present invention provides a method for monitoring of profile changes of components in a dynamic system such as a cell-free in vitro protein synthesis system by using liquid chromatography (LC) combined with mass spectrometry (MS). In an additional aspect, this invention provides a method for enhancing the yield and/or reproducibility in a cell-free protein synthesis system by modulating the level and/or activity of a protein component that has regulatory effects on the system.

Abstract translation: 本发明提供了一种用于通过使用液相色谱（LC）与质谱（MS）组合来监测动态系统（例如无细胞体外蛋白质合成系统）中组分的分布变化的方法。 在另一方面，本发明提供了通过调节对系统具有调节作用的蛋白质组分的水平和/或活性来提高无细胞蛋白质合成系统的产量和/或再现性的方法。

公开(公告)号：US20060019350A1

公开(公告)日：2006-01-26

申请号：US11244119

申请日：2005-10-04

Applicant: Peter Palese ,  Adolfo Garcia-Sastre

Inventor： Peter Palese ,  Adolfo Garcia-Sastre

IPC: C12N15/867 ,  C12P21/06 ,  C12N7/00

CPC classification number: C07K14/005 ,  A61K39/00 ,  A61K2039/5256 ,  C12N9/1247 ,  C12N9/127 ,  C12N15/10 ,  C12N15/11 ,  C12N15/86 ,  C12N2740/16122 ,  C12N2760/16122 ,  C12Y207/07006 ,  C12Y302/01018

Abstract: Recombinant negative-strand viral RNA templates are described which may be used with purified RNA-directed RNA polymerase complex to express heterologous gene products in appropriate host cells and/or to rescue the heterologous gene in virus particles. The RNA templates are prepared by transcription of appropriate DNA sequences with a DNA-directed RNA polymerase. The resulting RNA templates are of the negative-polarity and contain appropriate terminal sequences which enable the viral RNA-synthesizing apparatus to recognize the template. Bicistronic mRNAs can be constructed to permit internal initiation of translation of viral sequences and allow for the expression of foreign protein coding sequences from the regular terminal initiation site, or vice versa

Abstract translation: 描述了重组负链病毒RNA模板，其可以与纯化的RNA定向RNA聚合酶复合物一起使用以在合适的宿主细胞中表达异源基因产物和/或拯救病毒颗粒中的异源基因。 通过用DNA指导的RNA聚合酶转录适当的DNA序列来制备RNA模板。 得到的RNA模板是负极性的并且含有适当的末端序列，使得病毒RNA合成装置可以识别模板。 可以构建双顺反子mRNA以允许内部引发病毒序列的翻译，并允许外源蛋白编码序列从常规终止起始位点的表达，反之亦然

公开(公告)号：US20040098761A1

公开(公告)日：2004-05-20

申请号：US10616390

申请日：2003-07-09

Applicant: Kansas State University Research Foundation

Inventor： Harold N. Trick ,  Judith L. Roe ,  Timothy C. Todd ,  Michael A. Herman

IPC: A01H001/00 ,  C12N015/82

CPC classification number: C12N9/1051 ,  C07K14/4354 ,  C12N9/1241 ,  C12N15/8285 ,  C12Y204/01016 ,  C12Y207/07006 ,  Y02A40/164

Abstract: The present invention relates to compositions and methods for controlling nematode infestation of plants or animals. In particular, the present invention provides vectors comprising sequences designed to control nematodes by RNA interference (RNAi) and transgenic plants transformed with such vectors.

Abstract translation: 本发明涉及用于控制植物或动物的线虫侵袭的组合物和方法。 特别地，本发明提供了包含设计用于通过RNA干扰（RNAi）控制线虫的序列和用这些载体转化的转基因植物的载体。

公开(公告)号：US20240318219A1

公开(公告)日：2024-09-26

申请号：US18524159

申请日：2023-11-30

Applicant: Oxford Nanopore Technologies PLC

Inventor： John Milton ,  Sobia Nayyar ,  Jan Riedl ,  Ryosuke Ogaki

IPC: C12P19/34 ,  B01J19/00 ,  C12N9/12 ,  C12N15/10

CPC classification number: C12P19/34 ,  B01J19/0046 ,  C12N9/1247 ,  C12N9/1252 ,  C12N15/1093 ,  C12Y207/07006 ,  C12Y207/07007 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00722

Abstract: The invention relates to new methods for synthesising polynucleotide molecules according to a predefined nucleotide sequence. The invention also relates to methods for the assembly of synthetic polynucleotides following synthesis, as well as systems and kits for performing the synthesis and/or assembly methods.

公开(公告)号：US20240124863A1

公开(公告)日：2024-04-18

申请号：US18365105

申请日：2023-08-03

Applicant: President and Fellows of Harvard College

Inventor： David R. Liu ,  Bryan Dickinson ,  Michael S. Packer ,  Ahmed Hussein Badran

IPC: C12N9/50 ,  C12N7/00 ,  C12N9/12 ,  C12N15/10 ,  C12N15/52 ,  C12N15/62 ,  C12N15/73 ,  C12N15/86

CPC classification number: C12N9/50 ,  C12N7/00 ,  C12N9/1247 ,  C12N15/1037 ,  C12N15/1058 ,  C12N15/52 ,  C12N15/62 ,  C12N15/73 ,  C12N15/86 ,  C12Y207/07006 ,  C12Y304/00 ,  C07K2319/50 ,  C12N2795/00022

Abstract: Some aspects of this disclosure provide methods for phage-assisted continuous evolution (PACE) of proteases. Some aspects of this invention provide methods for evaluating and selecting protease inhibitors based on the likelihood of the emergence of resistant proteases as determined by the protease PACE methods provided herein. Some aspects of this disclosure provide strategies, methods, and reagents for protease PACE, including fusion proteins for translating a desired protease activity into a selective advantage for phage particles encoding a protease exhibiting such an activity and improved mutagenesis-promoting expression constructs. Evolved proteases that recognize target cleavage sites which differ from their canonical cleavage site are also provided herein.

公开(公告)号：US20240043899A1

公开(公告)日：2024-02-08

申请号：US18320389

申请日：2023-05-19

Applicant: SYNVITROBIO, INC.

Inventor： Zachary Z. Sun ,  Abel C. Chiao ,  Dan E. Robertson ,  Louis E. Metzger ,  Richard Mansfield ,  Kelly S. Trego

IPC: C12P21/02 ,  C12N15/85

CPC classification number: C12P21/02 ,  C12N15/85 ,  C12Y207/07006

Abstract: Provided herein, in one aspect, is a composition for in vitro transcription and translation, comprising: a treated cell lysate derived from one or more host cells such as bacteria, archaea, plant or animal; a plurality of supplements for gene transcription and translation; an energy recycling system for providing and recycling adenosine triphosphate (ATP); and one or more exogenous additives. Methods for making and using the same are also provided.

公开(公告)号：US20240035059A1

公开(公告)日：2024-02-01

申请号：US18255645

申请日：2021-12-01

Applicant: National University of Singapore

Inventor： Wai Kit Chee ,  Jing Wui Yeoh ,  Viet Linh Dao ,  Chueh Loo Poh

IPC: C12P19/34 ,  C12N15/70 ,  C12N9/12

CPC classification number: C12P19/34 ,  C12N15/70 ,  C12N9/1247 ,  C12Y207/07006 ,  C12N2800/101

Abstract: The present invention relates to heat-repressible split-T7 polymerases comprising temperature-sensitive domains of Tlpa protein fused with split T7 RNA polymerase (T7RNAP) to introduce thermal control into widely used T7 RNA polymerase, creating a heat-repressible Thermal-T7RNAP system. The invention further provides polynucleotides encoding the heat-repressible split-T7 polymerases, and methods of thermal control of bioproduction.

公开(公告)号：US20240018514A1

公开(公告)日：2024-01-18

申请号：US18065952

申请日：2022-12-14

Applicant: Translate Bio, Inc.

Inventor： Jonathan Abysalh ,  Daniel Crawford ,  Frank DeRosa ,  Anusha Dias ,  Michael Heartlein

IPC: C12N15/10 ,  C12P19/34 ,  C12N9/12 ,  G01N27/447

CPC classification number: C12N15/1096 ,  C12Y207/07006 ,  G01N27/44778 ,  C12N9/1247 ,  C12N15/1068 ,  C12P19/34

Abstract: The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.

公开(公告)号：US11866754B2

公开(公告)日：2024-01-09

申请号：US15768206

申请日：2016-10-17

Applicant: ModernaTX, Inc.

Inventor： Gabor Butora ,  Matthew Stanton ,  Edward John Miracco

IPC: C12P19/34 ,  C07H21/02 ,  A61K31/7125 ,  C12N9/12 ,  C12N15/10 ,  C12N15/113

CPC classification number: C12P19/34 ,  A61K31/7125 ,  C07H21/02 ,  C12N9/1247 ,  C12N15/1096 ,  C12N15/113 ,  C12N2310/317 ,  C12Y207/07006

Abstract: The present disclosure relates to cap analogs, which can result in high levels of capping efficiency and transcription and improved translation efficiencies. The present disclosure also relates to methods useful for preparing cap analogs and using mRNA species containing such analogs, as well as kits containing the novel cap analogs.