公开(公告)号：US20230193337A1

公开(公告)日：2023-06-22

申请号：US16757181

申请日：2018-10-19

Applicant: Synvitrobio, Inc.

Inventor： Zachary Z. Sun ,  Dan E. Robertson ,  Kelly S. Trego ,  Abel C. Chiao, IV ,  Louis E. Metzger, IV

IPC: C12P21/00 ,  C12N9/08 ,  C12N9/04

CPC classification number: C12P21/00 ,  C12N9/0065 ,  C12Y111/01006 ,  C12N9/0006 ,  C12Y101/0301

Abstract: The present disclosure relates to cell-free compositions and methods for making and using the same. In one aspect, the composition includes: an extract derived from one or more organisms; one or more proteins of interest, wherein the one or more proteins are expressed from one or more nucleic acids exogenous to the extract and/or by the one or more organisms, wherein preferably the one or more proteins react with a substrate to produce a product; and one or more O2, O—, or H2O2 scavengers. The composition may be oxygen-deprived. The composition may also include an energy recycling system.

公开(公告)号：US20240043899A1

公开(公告)日：2024-02-08

申请号：US18320389

申请日：2023-05-19

Applicant: SYNVITROBIO, INC.

Inventor： Zachary Z. Sun ,  Abel C. Chiao ,  Dan E. Robertson ,  Louis E. Metzger ,  Richard Mansfield ,  Kelly S. Trego

IPC: C12P21/02 ,  C12N15/85

CPC classification number: C12P21/02 ,  C12N15/85 ,  C12Y207/07006

Abstract: Provided herein, in one aspect, is a composition for in vitro transcription and translation, comprising: a treated cell lysate derived from one or more host cells such as bacteria, archaea, plant or animal; a plurality of supplements for gene transcription and translation; an energy recycling system for providing and recycling adenosine triphosphate (ATP); and one or more exogenous additives. Methods for making and using the same are also provided.

公开(公告)号：US20200181670A1

公开(公告)日：2020-06-11

申请号：US16638272

申请日：2018-08-13

Applicant: Synvitrobio, Inc.

Inventor： Zachary Z. Sun ,  Abel C. Chiao ,  Dan E. Robertson ,  Louis E. Metzger, IV ,  Richard Mansfield ,  Kelly S. Trego

IPC: C12P21/02 ,  C12N15/85

Abstract: Provided herein, in one aspect, is a composition for in vitro transcription and translation, comprising: a treated cell lysate derived from one or more host cells such as bacteria, archaea, plant or animal; a plurality of supplements for gene transcription and translation; an energy recycling system for providing and recycling adenosine triphosphate (ATP); and one or more exogenous additives. Methods for making and using the same are also provided.